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  4. Tetrazine-Amine monohydrochloride

Tetrazine-Amine monohydrochloride  (Synonyms: (4-(1,2,4,5-Tetrazin-3-yl)phenyl)methanamine monohydrochloride; Tetrazine-NH2 monohydrochloride)

Cat. No.: HY-W053709A Purity: 93.18%
COA Handling Instructions

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Tetrazine-Amine monohydrochloride is the monohydrochloride form of Tetrazine-Amine (HY-W053709). Tetrazine-amine is a Tetrazine linker that can be used to covalently label living cells by cycloaddition. Tetrazine-Amine (monohydrochloride) is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

For research use only. We do not sell to patients.

Tetrazine-Amine monohydrochloride Chemical Structure

Tetrazine-Amine monohydrochloride Chemical Structure

CAS No. : 1416711-59-5

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25 mg USD 70 In-stock
50 mg USD 110 In-stock
100 mg USD 180 In-stock
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Tetrazine-Amine monohydrochloride Related Antibodies

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Description

Tetrazine-Amine monohydrochloride is the monohydrochloride form of Tetrazine-Amine (HY-W053709). Tetrazine-amine is a Tetrazine linker that can be used to covalently label living cells by cycloaddition[1][2]. Tetrazine-Amine (monohydrochloride) is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

Molecular Weight

223.66

Formula

C9H10ClN5
CAS No.
Appearance

Solid

Color

Purple to purplish red

SMILES

NCC1=CC=C(C2=NN=CN=N2)C=C1.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (447.11 mM)

DMSO : 62.5 mg/mL (279.44 mM; ultrasonic and warming and heat to 70°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4711 mL 22.3554 mL 44.7107 mL
5 mM 0.8942 mL 4.4711 mL 8.9421 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.93%

COA (247 KB) HNMR (245 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 4.4711 mL 22.3554 mL 44.7107 mL 111.7768 mL
5 mM 0.8942 mL 4.4711 mL 8.9421 mL 22.3554 mL
10 mM 0.4471 mL 2.2355 mL 4.4711 mL 11.1777 mL
15 mM 0.2981 mL 1.4904 mL 2.9807 mL 7.4518 mL
20 mM 0.2236 mL 1.1178 mL 2.2355 mL 5.5888 mL
25 mM 0.1788 mL 0.8942 mL 1.7884 mL 4.4711 mL
30 mM 0.1490 mL 0.7452 mL 1.4904 mL 3.7259 mL
40 mM 0.1118 mL 0.5589 mL 1.1178 mL 2.7944 mL
50 mM 0.0894 mL 0.4471 mL 0.8942 mL 2.2355 mL
60 mM 0.0745 mL 0.3726 mL 0.7452 mL 1.8629 mL
80 mM 0.0559 mL 0.2794 mL 0.5589 mL 1.3972 mL
100 mM 0.0447 mL 0.2236 mL 0.4471 mL 1.1178 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Tetrazine-Amine monohydrochloride Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tetrazine-Amine monohydrochloride1416711-59-5(4-(1,2,4,5-Tetrazin-3-yl)phenyl)methanamine monohydrochlorideTetrazine-NH2 monohydrochlorideBiochemical Assay ReagentsInhibitorinhibitorinhibit

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