Vacquinol-1 dihydrochloride
Based on 1 Customer Validation
Vacquinol-1 (NSC13316) dihydrochloride is a MKK4-specific activator that activates the MAPK pathway. Vacquinol-1 dihydrochloride inhibits the growth, migration and colony formation, and induces apoptosis of cancer cells. Vacquinol-1 dihydrochloride is applicable to research related to cancers such as hepatocellular carcinoma.
For research use only. We do not sell to patients.
- Purity: 99.70%
- CAS No.: 2309312-85-2
- Formula: C21H23Cl3N2O
- Molecular Weight:425.78
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Vacquinol-1 (1.56-25 μM; 24-72 h) dihydrochloride inhibits the viability of BEL7402/Huh7 hepatocellular carcinoma cells in vitro[1].
Vacquinol-1 (0-24 μM; 24 h) dihydrochloride inhibits the colony-forming ability of BEL7402/Huh7 hepatocellular carcinoma cells and induces cell apoptosis[1].
Vacquinol-1 (10 μM; 24 h) dihydrochloride activates the endogenous apoptotic pathway in BEL7402 hepatocellular carcinoma cells, induces the cleavage of caspase-3, caspase-9 and PARP-1, upregulates the expression of Bax and Bim, and downregulates the expression of Bcl-2[1].
Vacquinol-1 (10 μM; 1-12 h) dihydrochloride induces the translocation of the pro-apoptotic protein Bax from the cytoplasm to mitochondria in BEL7402 hepatocellular carcinoma cells[1].
Vacquinol-1 (15 μM; 5 h) dihydrochloride activates the JNK and p38 MAPK pathways, upregulates α-SMA, an activation marker of the hepatic stellate cell line HSC-T6, promotes collagen production, and induces phosphorylation of the linker region of Smad2/3[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BEL7402 (hepatocellular carcinoma, HCC)
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Concentration:1.56 μM, 3.13 μM, 6.26 μM, 12.5 μM, 25 μM
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Incubation Time:24, 48, 72 h
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Result:Inhibited BEL7402 cell proliferation in a dose-dependent manner.
Reached half maximal inhibitory concentration (IC50) values of 12.35 μM (24 h), 11.48 μM (48 h), and 10.21 μM (72 h).
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Cell Line:Huh7 (hepatocellular carcinoma, HCC)
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Concentration:1.56 μM, 3.13 μM, 6.26 μM, 12.5 μM, 25 μM
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Incubation Time:24, 48, 72 h
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Result:Inhibited Huh7 cell proliferation in a dose-dependent manner.
Reached half maximal inhibitory concentration (IC50) values of 6.21 μM (24 h), 5.58 μM (48 h), and 4.89 μM (72 h).
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Cell Line:BEL7402 (hepatocellular carcinoma, HCC)
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Concentration:10 μM
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Incubation Time:24 h
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Result:Increased the percentage of apoptotic cells.
Resulted in nuclear fragmentation and apoptotic body formation in BEL7402 cells, whereas control cells displayed round, normal nuclei.
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Cell Line:rat hepatic stellate HSC-T6 cells
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Concentration:15 μM
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Incubation Time:5 h
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Result:Upregulated the protein levels of phosphorylated (P)-JNK1/2 and P-p38 (but not P-ERK1/2) in HSC-T6 cells.
Increased the protein levels of α-SMA, Collagen I, P-Smad2L, and P-Smad3L in HSC-T6 cells.
Chemical Information
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CAS No. 2309312-85-2
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Appearance Solid
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Molecular Weight 425.78
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Formula C21H23Cl3N2O
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Color White to off-white
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SMILES
OC(C1NCCCC1)C2=CC(C3=CC=C(Cl)C=C3)=NC4=CC=CC=C24.[H]Cl.[H]Cl
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Synonyms
NSC13316 dihydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 25 mg/mL (58.72 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 10 mg/mL (23.49 mM); Clear solution; Need ultrasonic and warming and heat to 44°C
Purity & Documentation
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Data Sheet (283 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Sun SL, Li X, Su N, et al. Vacquinol‑1 induces apoptosis in hepatocellular carcinoma cell. Mol Med Rep. 2018;18(1):557-563. [Content Brief]
[2]. Sander P, Mostafa H, Soboh A, et al. Vacquinol-1 inducible cell death in glioblastoma multiforme is counter regulated by TRPM7 activity induced by exogenous ATP. Oncotarget. 2017;8(21):35124-35137. [Content Brief]
[3]. Chao Wu, et al. Salvianolic acid B exerts anti-liver fibrosis effects via inhibition of MAPK-mediated phospho-Smad2/3 at linker regions in vivo and in vitro. Life Sci. 2019 Dec 15;239:116881. [Content Brief]
[4]. Yufeng Shi, et al. The soft underbelly of tumor cells. Cell Res. 2014 Aug;24(8):910-1. [Content Brief]
[5]. Shu-Lan Sun, et al. Vacquinol 1 induces apoptosis in hepatocellular carcinoma cell. Mol Med Rep. 2018 Jul;18(1):557-563. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3486 mL | 11.7432 mL | 23.4863 mL | 58.7158 mL |
| 5 mM | 0.4697 mL | 2.3486 mL | 4.6973 mL | 11.7432 mL | |
| 10 mM | 0.2349 mL | 1.1743 mL | 2.3486 mL | 5.8716 mL | |
| 15 mM | 0.1566 mL | 0.7829 mL | 1.5658 mL | 3.9144 mL | |
| 20 mM | 0.1174 mL | 0.5872 mL | 1.1743 mL | 2.9358 mL | |
| 25 mM | 0.0939 mL | 0.4697 mL | 0.9395 mL | 2.3486 mL | |
| 30 mM | 0.0783 mL | 0.3914 mL | 0.7829 mL | 1.9572 mL | |
| 40 mM | 0.0587 mL | 0.2936 mL | 0.5872 mL | 1.4679 mL | |
| 50 mM | 0.0470 mL | 0.2349 mL | 0.4697 mL | 1.1743 mL |
Vacquinol-1 dihydrochloride Related Classifications
HY-19345A Related Classifications
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