ZIF-8  (Synonyms: 2-Methylimidazole zinc salt)

ZIF-8 (2-Methylimidazole zinc salt) is a pyroptosis inducer that activates the caspase-1/gasdermin D-dependent pyroptosis pathway. ZIF-8 induces pyroptotic cell death accompanied by necrosis and immunogenic cell death. ZIF-8 initiates in situ immunity, activates anti-tumor immunity, and reprograms the immunosuppressive tumor microenvironment to inhibit tumor growth. ZIF-8 acts as a pH-responsive and stimulus-responsive drug release inducer. ZIF-8 is applicable to cancer-related research^[1][2].

ZIF-8 (2-Methylimidazole zinc salt) nanoparticles (0-24 h) remain stable in neutral pH (7.4) PBS but degrade and release ~53% of zinc ions within 24 h in acidic pH (6.5) PBS^[1].
ZIF-8 nanoparticles induce significant intracellular ROS generation in 4T1 mouse breast cancer cells^[1].
ZIF-8 nanoparticles induce significant intracellular zinc ion accumulation in 4T1 mouse breast cancer cells^[1].
ZIF-8 nanoparticles (25-50 μg/mL) exert concentration-dependent cytotoxicity against 4T1 mouse breast cancer cells, with an IC₅₀ of 23.1 μg/mL^[1].
ZIF-8 nanoparticles induce apoptosis and 31.4% necrosis in 4T1 mouse breast cancer cells^[1].
ZIF-8 nanoparticles (24 h) induce pyroptotic morphological changes and mitochondrial damage in 4T1 mouse breast cancer cells after 24 h of treatment^[1].
ZIF-8 nanoparticles upregulate cleaved caspase-1 and GSDMD expression to activate the caspase-1/GSDMD-dependent pyroptosis pathway in 4T1 mouse breast cancer cells^[1].
ZIF-8 nanoparticles induce significant release of pyroptosis markers LDH and IL-1β from 4T1 mouse breast cancer cells^[1].
ZIF-8 nanoparticles (20 μg/mL) induce immunogenic cell death in 4T1 mouse breast cancer cells, as evidenced by CRT exposure and ATP release at a concentration of 20 μg/mL^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

227.59

C₈H₁₀N₄Zn

59061-53-9

Solid

White to off-white

CC1=NC=CN1[Zn]N2C=CN=C2C

Room temperature in continental US; may vary elsewhere.

RT, stored under nitrogen

• [1]. Ding B, et al. ZIF-8 Nanoparticles Evoke Pyroptosis for High-Efficiency Cancer Immunotherapy. Angew Chem Int Ed Engl. 2023;62(10):e202215307.  [Content Brief]

  [2]. Wang Q, et al. Synthesis and modification of ZIF-8 and its application in drug delivery and tumor therapy. RSC Adv. 2020;10(62):37600-37620. Published 2020 Oct 12.