2,2-Dibromo-2-cyanoacetamide
Based on 1 Customer Validation
2,2-Dibromo-2-cyanoacetamide is a non-oxidizing bactericide. 2,2-Dibromo-2-cyanoacetamide enhances bactericidal activity by reacting with sulfhydryl groups on the cell walls of microorganisms in aquatic environments to release bromide ions. 2,2-Dibromo-2-cyanoacetamide effectively inactivates Legionella pneumophila, rendering it non-culturable, and exhibits significantly higher killing efficacy against this bacterium in reservoir biofilms than in aerosol biofilms. 2,2-Dibromo-2-cyanoacetamide is suitable for legionellosis prevention and control as well as related research.
For research use only. We do not sell to patients.
- Purity : 98.0%
- CAS No.: 10222-01-2
- Formula: C3H2Br2N2O
- Molecular Weight:241.87
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
2,2-Dibromo-2-cyanoacetamide (100 mg/L; 4 h) potently inactivates culturable Legionella pneumophila as well as naturally occurring cells in process water, aerosolized environmental biofilms, and reservoir water biofilms (with or without Tetrahymena thermophila)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 10222-01-2
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Appearance Solid
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Molecular Weight 241.87
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Formula C3H2Br2N2O
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SMILES
N#CC(Br)(Br)C(=O)N
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (413.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.34 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.34 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (952 KB)
- English - EN (952 KB)
- Français - FR (952 KB)
- Deutsch - DE (952 KB)
- Norwegian - NO (952 KB)
- Español - ES (952 KB)
- Swedish - SV (952 KB)
- Italian - IT (952 KB)
- Korean - KR (952 KB)
- Portuguese - PT (952 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.1345 mL | 20.6723 mL | 41.3445 mL | 103.3613 mL |
| 5 mM | 0.8269 mL | 4.1345 mL | 8.2689 mL | 20.6723 mL | |
| 10 mM | 0.4134 mL | 2.0672 mL | 4.1345 mL | 10.3361 mL | |
| 15 mM | 0.2756 mL | 1.3782 mL | 2.7563 mL | 6.8908 mL | |
| 20 mM | 0.2067 mL | 1.0336 mL | 2.0672 mL | 5.1681 mL | |
| 25 mM | 0.1654 mL | 0.8269 mL | 1.6538 mL | 4.1345 mL | |
| 30 mM | 0.1378 mL | 0.6891 mL | 1.3782 mL | 3.4454 mL | |
| 40 mM | 0.1034 mL | 0.5168 mL | 1.0336 mL | 2.5840 mL | |
| 50 mM | 0.0827 mL | 0.4134 mL | 0.8269 mL | 2.0672 mL | |
| 60 mM | 0.0689 mL | 0.3445 mL | 0.6891 mL | 1.7227 mL | |
| 80 mM | 0.0517 mL | 0.2584 mL | 0.5168 mL | 1.2920 mL | |
| 100 mM | 0.0413 mL | 0.2067 mL | 0.4134 mL | 1.0336 mL |
2,2-Dibromo-2-cyanoacetamide Related Classifications
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