2-Chloro-2′-β-C-methyladenosine
Based on 1 Customer Validation
2-Chloro-2′-β-C-methyladenosine is an adenosine analogue. Adenosine analogs mostly act as smooth muscle vasodilators and have also been shown to inhibit cancer progression. The popular products in this series are adenosine phosphate, Acadesine (HY-13417), Clofarabine (HY-A0005), Fludarabine phosphate (HY-B0028) and Vidarabine (HY-B0277).
For research use only. We do not sell to patients.
- Purity: 99.91%
- CAS No.: 205171-11-5
- Formula: C11H14ClN5O4
- Molecular Weight:315.71
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CCRF-CEM | CC50 |
>100 μM
Compound: 17
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Cytotoxicity against human CEM after 5 days
Cytotoxicity against human CEM after 5 days
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[PMID: 21983447] |
| CCRF-CEM | CC50 |
>100 μM
Compound: 17
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Cytotoxicity against human CEM cells after 6 days by trypan blue exclusion assay
Cytotoxicity against human CEM cells after 6 days by trypan blue exclusion assay
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[PMID: 26819659] |
| Huh-7 | CC50 |
>10 μM
Compound: 17
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Cytotoxicity against human HuH7 cells infected with HCV assessed as reduction in cellular rRNA level after 96 hrs by qRT-PCR analysis
Cytotoxicity against human HuH7 cells infected with HCV assessed as reduction in cellular rRNA level after 96 hrs by qRT-PCR analysis
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[PMID: 26819659] |
| PBMC | CC50 |
>100 μM
Compound: 17
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Cytotoxicity against human PBMC after 5 days
Cytotoxicity against human PBMC after 5 days
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[PMID: 21983447] |
| PBMC | CC50 |
>100 μM
Compound: 17
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Cytotoxicity against human PBMC after 6 days by trypan blue exclusion assay
Cytotoxicity against human PBMC after 6 days by trypan blue exclusion assay
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[PMID: 26819659] |
| Vero | CC50 |
>100 μM
Compound: 17
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Cytotoxicity against african green monkey Vero after 5 days
Cytotoxicity against african green monkey Vero after 5 days
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[PMID: 21983447] |
| Vero | CC50 |
>100 μM
Compound: 17
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Cytotoxicity against African green monkey Vero cells after 3 days by hemocytometry
Cytotoxicity against African green monkey Vero cells after 3 days by hemocytometry
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[PMID: 26819659] |
Chemical Information
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CAS No. 205171-11-5
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Appearance Solid
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Molecular Weight 315.71
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Formula C11H14ClN5O4
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Color White to off-white
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SMILES
OC[C@@H]1[C@@H](O)[C@](O)(C)[C@H](N2C=NC3=C(N)N=C(Cl)N=C32)O1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (158.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.59 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.59 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1675 mL | 15.8373 mL | 31.6746 mL | 79.1866 mL |
| 5 mM | 0.6335 mL | 3.1675 mL | 6.3349 mL | 15.8373 mL | |
| 10 mM | 0.3167 mL | 1.5837 mL | 3.1675 mL | 7.9187 mL | |
| 15 mM | 0.2112 mL | 1.0558 mL | 2.1116 mL | 5.2791 mL | |
| 20 mM | 0.1584 mL | 0.7919 mL | 1.5837 mL | 3.9593 mL | |
| 25 mM | 0.1267 mL | 0.6335 mL | 1.2670 mL | 3.1675 mL | |
| 30 mM | 0.1056 mL | 0.5279 mL | 1.0558 mL | 2.6396 mL | |
| 40 mM | 0.0792 mL | 0.3959 mL | 0.7919 mL | 1.9797 mL | |
| 50 mM | 0.0633 mL | 0.3167 mL | 0.6335 mL | 1.5837 mL | |
| 60 mM | 0.0528 mL | 0.2640 mL | 0.5279 mL | 1.3198 mL | |
| 80 mM | 0.0396 mL | 0.1980 mL | 0.3959 mL | 0.9898 mL | |
| 100 mM | 0.0317 mL | 0.1584 mL | 0.3167 mL | 0.7919 mL |
2-Chloro-2′-β-C-methyladenosine Related Classifications
HY-152812 Related Classifications
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