Mucic acid
Based on 1 Customer Validation
Mucic acid is an aldonic acid that can be prepared from pectin. Mucic acid increases the expression level of Runx2, promotes osteoblast differentiation, and upregulates osteogenic differentiation marker genes. Mucic acid can be used to prepare platform compounds, polymers, polymer materials and metal-organic frameworks. Mucic acid is applicable to the research of bone-related defects.
For research use only. We do not sell to patients.
- Purity : 99.36%
- CAS No.: 526-99-8
- Formula: C6H10O8
- Molecular Weight:210.14
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
RUNX2 |
In Vitro
Mucic acid (10 μM; 72 h) significantly stimulates the expression of Runx2 mRNA in C3H10T1/2 mouse mesenchymal stem cells[1].
Mucic acid, a plant-derived bioactive molecule that promotes osteogenic differentiation, can be incorporated into the gelatin coating of 3D-printed PLA scaffolds[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:C3H10T1/2 mouse mesenchymal stem cells (mMSCs)
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Concentration:10 μM
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Incubation Time:72 h
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Result:Significantly stimulated Runx2 mRNA expression compared to untreated controls.
Chemical Information
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CAS No. 526-99-8
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Appearance Solid
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Molecular Weight 210.14
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Formula C6H10O8
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Color White to off-white
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SMILES
O[C@H]([C@@H](O)C(O)=O)[C@@H](O)[C@H](O)C(O)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 25 mg/mL (118.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.90 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.90 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (283 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1].
Ashwin B, et al. 3D-poly (lactic acid) scaffolds coated with gelatin and mucic acid for bone tissue engineering. Int J Biol Macromol. 2020 Nov 1;162:523-532.
[Content Brief]
[2].
Tan H, et al. [Production of mucic acid from pectin-derived D-galacturonic acid: a review]. Sheng Wu Gong Cheng Xue Bao. 2022 Feb 25;38(2):666-677. Chinese.
[Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.7587 mL | 23.7937 mL | 47.5873 mL | 118.9683 mL |
| 5 mM | 0.9517 mL | 4.7587 mL | 9.5175 mL | 23.7937 mL | |
| 10 mM | 0.4759 mL | 2.3794 mL | 4.7587 mL | 11.8968 mL | |
| 15 mM | 0.3172 mL | 1.5862 mL | 3.1725 mL | 7.9312 mL | |
| 20 mM | 0.2379 mL | 1.1897 mL | 2.3794 mL | 5.9484 mL | |
| 25 mM | 0.1903 mL | 0.9517 mL | 1.9035 mL | 4.7587 mL | |
| 30 mM | 0.1586 mL | 0.7931 mL | 1.5862 mL | 3.9656 mL | |
| 40 mM | 0.1190 mL | 0.5948 mL | 1.1897 mL | 2.9742 mL | |
| 50 mM | 0.0952 mL | 0.4759 mL | 0.9517 mL | 2.3794 mL | |
| 60 mM | 0.0793 mL | 0.3966 mL | 0.7931 mL | 1.9828 mL | |
| 80 mM | 0.0595 mL | 0.2974 mL | 0.5948 mL | 1.4871 mL | |
| 100 mM | 0.0476 mL | 0.2379 mL | 0.4759 mL | 1.1897 mL |
Mucic acid Related Classifications
HY-W014410 Related Classifications
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Keywords
- Mucic acid
- 526-99-8
- RUNX
- osteoblast differentiation
- D-galacturonic acid
- recombinant Trichoderma reesei QM6a Δgar1 udh
- C3H10T1/2 mouse mesenchymal stem cells
- osteogenic marker genes
- ALP activity
- recombinant Escherichia coli BL21 ΔuxacΔgarD udh
- mineralization
- recombinant Aspergillus niger ATCC 1015 ΔgaaaA udh
- Runx2
- Inhibitor
- inhibitor
- inhibit