Cat. No.: HY-W012814

4-Methylcatechol

Customer Review

Based on 1 Customer Validation

4-Methylcatechol is an intermediate in the degradation of some alkylbenzenes and an orally active suicide inhibitor of catechol 2,3-dioxygenase (C23O). 4-Methylcatechol induces apoptosis in melanoma cells through oxidative stress, but some studies have also shown that 4-Methylcatechol is carcinogenic. In addition, 4-Methylcatechol has antiplatelet and blood pressure-lowering activities. 4-Methylcatechol can also inhibit protein oxidation in beef but does not disulfide formation.

For research use only. We do not sell to patients.

Purity: 99.17%
CAS No.: 452-86-8
Formula: C₇H₈O₂
Molecular Weight:124.14

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

4-Methylcatechol Chemical Structure

CAS No.: 452-86-8

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Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Endogenous Metabolite Isoforms :

In Vitro

4-Methylcatechol (0-25 μg/mL; 8 h-14 days) can inhibit the proliferation of melanoma cells, induce cell cycle arrest and induce apoptosis through the generation of reactive oxygen species through mitochondrial pathway^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

4-Methylcatechol (Feed management; 2%; 104 weeks) is carcinogenic to the forestomach and glandular stomach in F344 rats^[2].
4-Methylcatechol (Intravenous injection; 0.2-25 mg/kg; single dose) has vasodilatory and blood pressure-lowering effects in spontaneously hypertensive rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male and female F344 rats aged 5 weeks old^[2]
Dosage:

2%
Administration:

Feed management; 104 weeks
Result:

Slightly reduced the average body weight and food intake in rats.
Decreased survival from about the 70th week in male rats, the cause of death may have been perforation due to glandular stomach ulceration.
Caused multiple nodules or large masses in the forestomachs in rats.
Caused thickening or polypoid lesions in the pyloric region in the glandular stomach in rats.
Induced forestomach papillomas and squamous-cell carcinomas, as well as glandular stomach submucosal hyperplasias, adenomas, and adenocarcinomas, some of which were accompanied by ulceration or erosion.

Chemical Information

CAS No. 452-86-8
Appearance Solid
Molecular Weight 124.14
Formula C₇H₈O₂
Color Off-white to light brown
SMILES

OC1=CC=C(C)C=C1O
Structure Classification
- Phenols
- Polyphenols
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (805.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 100 mg/mL (805.55 mM)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

8.0555 mL

40.2774 mL

80.5549 mL

5 mM

1.6111 mL

8.0555 mL

16.1110 mL

10 mM

0.8055 mL

4.0277 mL

8.0555 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (20.14 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (20.14 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (20.14 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Payton F, et al. 4-Methylcatechol-induced oxidative stress induces intrinsic apoptotic pathway in metastatic melanoma cells. Biochem Pharmacol. 2011 May 15;81(10):1211-8. [Content Brief]

[2]. Asakawa E, et al. Carcinogenicity of 4-methoxyphenol and 4-methylcatechol in F344 rats. Int J Cancer. 1994 Jan 2;56(1):146-52. [Content Brief]

[3]. Pourová J, et al. Two flavonoid metabolites, 3,4-dihydroxyphenylacetic acid and 4-methylcatechol, relax arteries ex vivo and decrease blood pressure in vivo. Vascul Pharmacol. 2018 Dec;111:36-43. [Content Brief]

[4]. Okuta A, et al. Construction of chimeric catechol 2,3-dioxygenase exhibiting improved activity against the suicide inhibitor 4-methylcatechol. Appl Environ Microbiol. 2004 Mar;70(3):1804-10. [Content Brief]

[5]. Jongberg S, et al. 4-methylcatechol inhibits protein oxidation in meat but not disulfide formation. J Agric Food Chem. 2011 Sep 28;59(18):10329-35. [Content Brief]

[6]. Applová L, et al. 4-Methylcatechol, a Flavonoid Metabolite with Potent Antiplatelet Effects. Mol Nutr Food Res. 2019 Aug 7;63(20):e1900261. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	8.0555 mL	40.2774 mL	80.5549 mL	201.3872 mL
	5 mM	1.6111 mL	8.0555 mL	16.1110 mL	40.2774 mL
	10 mM	0.8055 mL	4.0277 mL	8.0555 mL	20.1387 mL
	15 mM	0.5370 mL	2.6852 mL	5.3703 mL	13.4258 mL
	20 mM	0.4028 mL	2.0139 mL	4.0277 mL	10.0694 mL
	25 mM	0.3222 mL	1.6111 mL	3.2222 mL	8.0555 mL
	30 mM	0.2685 mL	1.3426 mL	2.6852 mL	6.7129 mL
	40 mM	0.2014 mL	1.0069 mL	2.0139 mL	5.0347 mL
	50 mM	0.1611 mL	0.8055 mL	1.6111 mL	4.0277 mL
	60 mM	0.1343 mL	0.6713 mL	1.3426 mL	3.3565 mL
	80 mM	0.1007 mL	0.5035 mL	1.0069 mL	2.5173 mL
	100 mM	0.0806 mL	0.4028 mL	0.8055 mL	2.0139 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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4-Methylcatechol

Bioactive Compound Library Max

Bioactive Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Oxygen Sensing Compound Library

Anti-Cardiovascular Disease Compound Library

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Gut Microbial Metabolite Library

Microbial Metabolite Library

Anti-Parkinson's Disease Compound Library

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Human Metabolite Library

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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

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Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Chinese Baijiu Compound Library

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Mass Spectrometry Natural Product Library

Drug Fragment Library

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

4-Methylcatechol Related Classifications

HY-W012814 Related Classifications