4-Nitrophenyl β-D-glucuronide
Based on 1 publication(s) in Google Scholar
4-Nitrophenyl β-D-glucuronide (pNPG_1) is aderivative of 4-Nitrophenol. 4-Nitrophenyl β-D-glucuronide is the chromogenic substrate for β-glucuronidase.
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 10344-94-2
- Formula: C12H13NO9
- Molecular Weight:315.23
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 4-Nitrophenyl β-D-glucuronide
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Biological Activity
4-Nitrophenyl β-D-glucuronide is the substrate for β-glucuronidase (β-GUS). 4-Nitrophenyl β-D-glucuronide can be hydrolyzed by β-GUS to generate blue-coloured 4-nitrophenol with electrochemical property, which can be used for quantitative detection of the β-GUS activity[2].
Since 97% of E. coli strains can produce β-GUS, 4-Nitrophenyl β-D-glucuronide can thus be used for E. coli quantitative detection[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 10344-94-2
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Appearance Solid
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Molecular Weight 315.23
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Formula C12H13NO9
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Color White to off-white
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SMILES
OC([C@H]([C@H]([C@@H]([C@H]1O)O)O)O[C@H]1OC2=CC=C(C=C2)[N+]([O-])=O)=O
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Synonyms
pNPG_1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (1)
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Journal Impact Factor
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Most Recent
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Food Chem X
2025 Sep 29:31:103103. PMID: 41080133
Solvent & Solubility
H2O : ≥ 100 mg/mL (317.23 mM)
DMSO : 100 mg/mL (317.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.93 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.93 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.1723 mL | 15.8614 mL | 31.7229 mL | 79.3072 mL |
| 5 mM | 0.6345 mL | 3.1723 mL | 6.3446 mL | 15.8614 mL | |
| 10 mM | 0.3172 mL | 1.5861 mL | 3.1723 mL | 7.9307 mL | |
| 15 mM | 0.2115 mL | 1.0574 mL | 2.1149 mL | 5.2871 mL | |
| 20 mM | 0.1586 mL | 0.7931 mL | 1.5861 mL | 3.9654 mL | |
| 25 mM | 0.1269 mL | 0.6345 mL | 1.2689 mL | 3.1723 mL | |
| 30 mM | 0.1057 mL | 0.5287 mL | 1.0574 mL | 2.6436 mL | |
| 40 mM | 0.0793 mL | 0.3965 mL | 0.7931 mL | 1.9827 mL | |
| 50 mM | 0.0634 mL | 0.3172 mL | 0.6345 mL | 1.5861 mL | |
| 60 mM | 0.0529 mL | 0.2644 mL | 0.5287 mL | 1.3218 mL | |
| 80 mM | 0.0397 mL | 0.1983 mL | 0.3965 mL | 0.9913 mL | |
| 100 mM | 0.0317 mL | 0.1586 mL | 0.3172 mL | 0.7931 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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