5,15-Diacetyl-3-benzoyllathyrol
Based on 1 Customer Validation
5,15-Diacetyl-3-benzoyllathyrol is one of the lathyrane diterpenoids, that has anti-cancer activity.
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- Purity: 99.85%
- CAS No.: 218916-52-0
- 화학식: C31H38O7
- 분자량:522.63
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보관:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| 4T1 | IC50 |
>10 μM
Compound: 1
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Cytotoxicity against mouse 4T1 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
Cytotoxicity against mouse 4T1 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
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[PMID: 34018743] |
| RAW264.7 | IC50 |
>50 μM
Compound: 7
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Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability after 24 hrs by CCK8 assay
Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability after 24 hrs by CCK8 assay
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[PMID: 30817151] |
| RAW264.7 | IC50 |
8.06 μM
Compound: Euphorbia factor L3
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Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 3 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based assay
Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 3 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based assay
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[PMID: 35063896] |
| RAW264.7 | IC50 |
8.06 μM
Compound: Euphorbia factor L3
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Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 3 hrs followed by LPS addition and measured after 24 hrs
Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 3 hrs followed by LPS addition and measured after 24 hrs
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[PMID: 37002536] |
| RAW264.7 | IC50 |
8.3 μM
Compound: 7
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Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 3 hrs followed by LPS-stimulation and measured after 24 hrs by Griess assay
Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 3 hrs followed by LPS-stimulation and measured after 24 hrs by Griess assay
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[PMID: 30817151] |
5,15-Diacetyl-3-benzoyllathyrol shows anticancer activity in vitro against lung cancer A549 cells and the IC50 values are 34.04±3.99 μM. Furthermore, 5,15-Diacetyl-3-benzoyllathyrol can induce apoptosis in A549 cells via the mitochondrial pathway including loss of mitochondrial potential and release of cytochrome c[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 218916-52-0
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Appearance Solid
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분자량 522.63
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화학식 C31H38O7
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Color White to off-white
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SMILES
CC(O[C@@]1(C(/C(C)=C\[C@@H](C2(C)C)[C@@H]2CCC3=C)=O)[C@]([C@H]3OC(C)=O)([H])[C@@H](OC(C4=CC=CC=C4)=O)[C@@H](C)C1)=O
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Synonyms
Euphorbia factor L3
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Structure Classification
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
용액&용해도
DMSO : 100 mg/mL (191.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Cells are harvested during logarithmic growth phase and seeded in 96-well plates at a density of 1.5×104 cells/mL of final volume 190 μL/well. After incubation of 24 h, 10 μL EFL3 solution of full range concentrations is added to 96-well plates. After 68 h treatment, 10 μL MTT of 10 mM is added to each well for 4 h of maintaining at 37°C. Then, the supernatant is removed and the crystals are dissolved with 100 μL anhydrous DMSO each well. Subsequently, cell viability is measured by Model 550 Microplate reader at 540 nm and 655 nm as reference filter. Experiments are carried out at least thrice. The 50% inhibitory concentration (IC50) is defined as the anticancer agent concentration causing 50% reduction in cell viability and calculated from the cytotoxicity curves. Cell survival is calculated with the following formula: survival (%)=(mean experimental absorbance/mean control absorbance) × 100%.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
순도&문서
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Data Sheet (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9134 mL | 9.5670 mL | 19.1340 mL | 47.8350 mL |
| 5 mM | 0.3827 mL | 1.9134 mL | 3.8268 mL | 9.5670 mL | |
| 10 mM | 0.1913 mL | 0.9567 mL | 1.9134 mL | 4.7835 mL | |
| 15 mM | 0.1276 mL | 0.6378 mL | 1.2756 mL | 3.1890 mL | |
| 20 mM | 0.0957 mL | 0.4783 mL | 0.9567 mL | 2.3917 mL | |
| 25 mM | 0.0765 mL | 0.3827 mL | 0.7654 mL | 1.9134 mL | |
| 30 mM | 0.0638 mL | 0.3189 mL | 0.6378 mL | 1.5945 mL | |
| 40 mM | 0.0478 mL | 0.2392 mL | 0.4783 mL | 1.1959 mL | |
| 50 mM | 0.0383 mL | 0.1913 mL | 0.3827 mL | 0.9567 mL | |
| 60 mM | 0.0319 mL | 0.1594 mL | 0.3189 mL | 0.7972 mL | |
| 80 mM | 0.0239 mL | 0.1196 mL | 0.2392 mL | 0.5979 mL | |
| 100 mM | 0.0191 mL | 0.0957 mL | 0.1913 mL | 0.4783 mL |
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