Cat. No.: HY-N0697

Crocin

(Synonyms: Crocin I)

16 Cited Publications

Customer Review

Based on 16 publication(s) in Google Scholar

Crocin (Crocin I) is an orally active natural product that can be isolated from the stigma of Crocus sativus. Crocin inhibits tumor cell proliferation and promotes apoptosis through JAK pathway. Crocin has anti-inflammatory, antioxidant and antitumor activities .

For research use only. We do not sell to patients.

Purity: 99.30%
CAS No.: 42553-65-1
Formula: C₄₄H₆₄O₂₄
Molecular Weight:976.96

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Crocin Chemical Structure

CAS No.: 42553-65-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (16)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All Endogenous Metabolite Isoforms :

All JAK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	>100 μM Compound: 16, crocin	Inhibition of nitric oxide production in lipopolysaccharide-activated mouse RAW264.7 cells by Griess reaction based method Inhibition of nitric oxide production in lipopolysaccharide-activated mouse RAW264.7 cells by Griess reaction based method	[PMID: 23305920]

In Vitro

Crocin (150, 200 μM, 24 h) plays an antitumor role in colon cancer cells by inhibiting the JAK pathway^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

HCT116
Concentration:

0, 50, 100, 150, 200, 250, 300, 350, 400, and 450 μM
Incubation Time:

24 h
Result:

Weakened the cell vitality in a concentration-dependent manner.

Apoptosis Analysis^[1]

Cell Line:

HCT116
Concentration:

150, 200 μM
Incubation Time:

24 h
Result:

Facilitated apoptosis of HCT-116 cells in a concentration-dependent manner.
Abated the colony number and downregulated the positive expression of Ki-67.

Western Blot Analysis^[1]

Cell Line:

HCT116
Concentration:

150, 200 μM
Incubation Time:

24 h
Result:

Decreased the levels of p-JAK2, p-STAT3, and p-ERK, as well as the ratios of p-JAK2/JAK2, p-STAT3/STAT3, and pERK/ERK.

In Vivo

Crocin (20 mg/kg, orally, for 5 consecutive weeks) shows significant antioxidant, anti-inflammatory and antifibrotic effects on Bleomycin (HY-108345) -induced pulmonary fibrosis in rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Bleomycin-induced pulmonary fibrosis in rat^[2]
Dosage:

20 mg/kg
Administration:

p.o.
Result:

Reduced the expression of TLR4 and IL-10.
Reduced the expression of TNF-α and TGF-β1.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 42553-65-1
Appearance Solid
Molecular Weight 976.96
Formula C₄₄H₆₄O₂₄
Color Brown to reddish brown
SMILES

O[C@@H]([C@H](O)[C@H]1O)[C@H](O[C@H]1OC(/C(C)=C/C=C/C(C)=C/C=C/C=C(C)/C=C/C=C(C)/C(O[C@@H]([C@@H]([C@@H](O)[C@@H]2O)O)O[C@@H]2CO[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3CO)=O)=O)CO[C@@H]([C@@H]([C@@H](O)[C@@H]4O)O)O[C@@H]4CO
Synonyms

Crocin I
Structure Classification
- Terpenoids
- Diterpenoids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (16)

All IHCIFHistological Imaging/StainingWB

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Journal Impact Factor

Biomed Pharmacother

Crocin-I mitigates diquat-induced pulmonary fibrosis via activation of the SIRT3/FOXO3a pathway. [Abstract]

2025 Apr 6:186:118043. PMID: 40194334

Crocin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2025 Apr 6:186:118043. [Abstract]

Representative immunohistochemical images of SIRT3 and FOXO3a expression intensity in each group. Immunohistochemical analysis indicated that Crocin (Crocin-I) (40 mg/kg; gavage; once daily for 3 weeks) treatment enhanced the positive expression of SIRT3 and FOXO3a in the lung tissues of DQ-treated mice.

Crocin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2025 Apr 6:186:118043. [Abstract]

Crocin (Crocin-I) (40 mg/kg; gavage; once daily for 3 weeks) markedly reversed the downregulated expression of SIRT3 and FOXO3a induced by fibrosis in DQ-triggered pulmonary fibrosis mice.

Crocin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2025 Apr 6:186:118043. [Abstract]

Representative hematoxylin and eosin and Masson-stained images illustrating the effect of crocin-I on the lung tissues of DQ-induced PF mice. HE staining and Masson staining confirmed that Crocin (Crocin-I) (40 mg/kg; gavage; once daily for 3 weeks) significantly reduced the severity of PF in DQ-intoxicated mice. Scale bars: 100 µm (upper) and 50 µm (lower).
Int J Mol Sci

Discovery of TRPV4-Targeting Small Molecules with Anti-Influenza Effects Through Machine Learning and Experimental Validation. [Abstract]

2025 Feb 6;26(3):1381. PMID: 39941149
Int J Mol Sci

Crocin Protects the 661W Murine Photoreceptor Cell Line against the Toxic Effects of All- Trans-Retinal. [Abstract]

2024 Sep 20;25(18):10124. PMID: 39337609
Biomolecules

Establishment of a Cell Line Stably Expressing the Growth Hormone Secretagogue Receptor to Identify Crocin as a Ghrelin Agonist. [Abstract]

2022 Dec 5;12(12):1813. PMID: 36551241

Crocin purchased from MedChemExpress. Usage Cited in: Biomolecules. 2022 Dec 5;12(12):1813. [Abstract]

Crocin (5, 10, 50,100 μM) significantly increases the level of ERK1/2 phosphorylation in GHSR1a-mCherry HEK293T cells.
Int Immunopharmacol

Rutin ameliorates hepatic ischemia-reperfusion injury by targeting CD36 to suppress hepatocyte ferroptosis. [Abstract]

2026 Mar 1:172:116231. PMID: 41576568
J Mol Struct

2025 Dec 15.
Int Immunopharmacol

Crocin facilitates peripheral nerve regeneration through modulation of the STAT3/Bcl-2/Beclin-1 signaling axis-mediated autophagic pathway. [Abstract]

2025 Sep 5:165:115437. PMID: 40913861

Crocin purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Sep 5:165:115437. [Abstract]

Hematoxylin and eosin (H&E) staining of transverse sections 28 days after surgery showed well-preserved tissue structure with no necrotic or scar tissue in the Crocin (50 mg/kg; i.p.; once daily; rats) group, whereas the control group exhibited sparse and disorganized axonal regeneration. (scale bar = 100 μm).

Crocin purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Sep 5:165:115437. [Abstract]

Immunofluorescence analysis showed that Schwann cell regeneration markers (S100β+) and axon regeneration markers (/NF200+) were significantly up-regulated in the Crocin (50 mg/kg; i.p.; once daily; rats) group compared with the control group.

Crocin purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Sep 5:165:115437. [Abstract]

Morphological analysis of the target muscle via Masson's trichrome staining revealed a significant reduction in collagen fiber area in the Crocin (50 mg/kg; i.p.; once daily; rats)-treated group compared to the crush injury group (scale bar = 100 μm).

Crocin purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Sep 5:165:115437. [Abstract]

Western blot analysis of NLRP3 inflammasome components, LC3B, and GSDMD under compound posttreatment. Western blot analysis demonstrated that the Crocin (50 mg/kg; i.p.; once daily; rats)-treated group exhibited significant suppression of NLRP3-mediated pyroptosis-related proteins (including NLRP3, IL-1β, caspase-1, and GSDMD) alongside elevated LC3B expression.
Bioengineered

Crocin exerts anti-proliferative and apoptotic effects on cutaneous squamous cell carcinoma via miR-320a/ATG2B. [Abstract]

2021 Dec;12(1):4569-4580. PMID: 34320900
Cytokine

Crocin attenuates NF-κB-mediated inflammation and proliferation in breast cancer cells by down-regulating PRKCQ. [Abstract]

2022 Jun;154:155888. PMID: 35447530
Cell Cycle

Crocin ameliorates atherosclerosis by promoting the reverse cholesterol transport and inhibiting the foam cell formation via regulating PPARγ/LXR-α. [Abstract]

2022 Jan;21(2):202-218. PMID: 34978526
Chem Biol Drug Des

Crocin enhances the sensitivity to paclitaxel in human breast cancer cells by reducing BIRC5 expression. [Abstract]

2024 Feb;103(2):e14467. PMID: 38661582
Ren Fail

Crocin improves the renal autophagy in rat experimental membranous nephropathy via regulating the SIRT1/Nrf2/HO-1 signaling pathway. [Abstract]

2023;45(2):2253924. PMID: 37724538
J Phys Chem B

Nonconjugated Substituents Modulate Excited-State Dynamics of Keto-Carotenoids. [Abstract]

2026 Jan 8;130(1):205-214. PMID: 41404815
Mol Cell Toxicol

2025 Dec 10.
Pharm Chem J

2024 May 15.
SSRN

2023 Dec 19.

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (127.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.0236 mL

5.1179 mL

10.2358 mL

5 mM

0.2047 mL

1.0236 mL

2.0472 mL

10 mM

0.1024 mL

0.5118 mL

1.0236 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (2.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (2.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zaghloul MS, et al. Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosis. Life Sci. 2019 Aug 26:116794. [Content Brief]

[2]. Yang H, et al. Crocin exerts anti-tumor effect in colon cancer cells via repressing the JAK pathway. Eur J Histochem. 2023 Sep 12;67(3):3697. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.0236 mL	5.1179 mL	10.2358 mL	25.5896 mL
	5 mM	0.2047 mL	1.0236 mL	2.0472 mL	5.1179 mL
	10 mM	0.1024 mL	0.5118 mL	1.0236 mL	2.5590 mL
	15 mM	0.0682 mL	0.3412 mL	0.6824 mL	1.7060 mL
	20 mM	0.0512 mL	0.2559 mL	0.5118 mL	1.2795 mL
	25 mM	0.0409 mL	0.2047 mL	0.4094 mL	1.0236 mL
	30 mM	0.0341 mL	0.1706 mL	0.3412 mL	0.8530 mL
	40 mM	0.0256 mL	0.1279 mL	0.2559 mL	0.6397 mL
	50 mM	0.0205 mL	0.1024 mL	0.2047 mL	0.5118 mL
	60 mM	0.0171 mL	0.0853 mL	0.1706 mL	0.4265 mL
	80 mM	0.0128 mL	0.0640 mL	0.1279 mL	0.3199 mL
	100 mM	0.0102 mL	0.0512 mL	0.1024 mL	0.2559 mL

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Crocin

Apoptosis Compound Library

Epigenetics Compound Library

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JAK/STAT Compound Library

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Publications Citing Use of MedChemExpress (MCE) Crocin

All Endogenous Metabolite Isoforms

All JAK Isoforms

Biological Activity

Chemical Information

Publications (16)

Solvent & Solubility

Purity & Documentation