5'-Phosphoguanylyl-(3',5')-guanosine  (Synonyms: pGpG)

5'-Phosphoguanylyl-(3',5')-guanosine (pGpG) is an intermediate molecule produced by the pathway for enzymatic cyclic diguanylate (c-di-GMP) degradation. 5'-Phosphoguanylyl-(3',5')-guanosine can be used to detect the metabolism of nucleic acids^[1][2].

5'-Phosphoguanylyl-(3',5')-guanosine reduces the rate of c-di-GMP degradation in cell lysates and inhibits the activity of EAL-dependent PDEs (PA2133, PvrR, and purified recombinant RocR) from P. aeruginosa^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

708.43

C₂₀H₂₆N₁₀O₁₅P₂

33008-99-0

Solid

White to off-white

O=C1C2=C(N([C@H]3[C@H](O)[C@H](O)[C@@H](COP(O)(O[C@H]4[C@@H](O)[C@H](N(C=N5)C6=C5C(N=C(N)N6)=O)O[C@@H]4COP(O)(O)=O)=O)O3)C=N2)NC(N)=N1

Room temperature in continental US; may vary elsewhere.

-20°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Nesbitt NM, et, al. Modification of a bi-functional diguanylate cyclase-phosphodiesterase to efficiently produce cyclic diguanylate monophosphate. Biotechnol Rep (Amst). 2015 May 5;7:30-37.  [Content Brief]

  [2]. Cohen D, et, al. Oligoribonuclease is a central feature of cyclic diguanylate signaling in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2015 Sep 8;112(36):11359-64.  [Content Brief]