5'-Phosphoguanylyl-(3',5')-guanosine
Based on 1 Customer Validation
5'-Phosphoguanylyl-(3',5')-guanosine (pGpG) is an intermediate molecule produced by the pathway for enzymatic cyclic diguanylate (c-di-GMP) degradation. 5'-Phosphoguanylyl-(3',5')-guanosine can be used to detect the metabolism of nucleic acids.
For research use only. We do not sell to patients.
- Purity : 95.77%
- CAS No.: 33008-99-0
- Formula: C20H26N10O15P2
- Molecular Weight:708.43
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Storage:
-20°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
5'-Phosphoguanylyl-(3',5')-guanosine reduces the rate of c-di-GMP degradation in cell lysates and inhibits the activity of EAL-dependent PDEs (PA2133, PvrR, and purified recombinant RocR) from P. aeruginosa[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 33008-99-0
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Appearance Solid
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Molecular Weight 708.43
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Formula C20H26N10O15P2
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Color White to off-white
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SMILES
O=C1C2=C(N([C@H]3[C@H](O)[C@H](O)[C@@H](COP(O)(O[C@H]4[C@@H](O)[C@H](N(C=N5)C6=C5C(N=C(N)N6)=O)O[C@@H]4COP(O)(O)=O)=O)O3)C=N2)NC(N)=N1
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Synonyms
pGpG
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Solvent & Solubility
In Vitro:
H2O : 50 mg/mL (70.58 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Nesbitt NM, et, al. Modification of a bi-functional diguanylate cyclase-phosphodiesterase to efficiently produce cyclic diguanylate monophosphate. Biotechnol Rep (Amst). 2015 May 5;7:30-37. [Content Brief]
[2]. Cohen D, et, al. Oligoribonuclease is a central feature of cyclic diguanylate signaling in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2015 Sep 8;112(36):11359-64. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.4116 mL | 7.0579 mL | 14.1157 mL | 35.2893 mL |
| 5 mM | 0.2823 mL | 1.4116 mL | 2.8231 mL | 7.0579 mL | |
| 10 mM | 0.1412 mL | 0.7058 mL | 1.4116 mL | 3.5289 mL | |
| 15 mM | 0.0941 mL | 0.4705 mL | 0.9410 mL | 2.3526 mL | |
| 20 mM | 0.0706 mL | 0.3529 mL | 0.7058 mL | 1.7645 mL | |
| 25 mM | 0.0565 mL | 0.2823 mL | 0.5646 mL | 1.4116 mL | |
| 30 mM | 0.0471 mL | 0.2353 mL | 0.4705 mL | 1.1763 mL | |
| 40 mM | 0.0353 mL | 0.1764 mL | 0.3529 mL | 0.8822 mL | |
| 50 mM | 0.0282 mL | 0.1412 mL | 0.2823 mL | 0.7058 mL | |
| 60 mM | 0.0235 mL | 0.1176 mL | 0.2353 mL | 0.5882 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
5'-Phosphoguanylyl-(3',5')-guanosine Related Classifications
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