Dibutyl phthalate

Name: Dibutyl phthalate
Brand: MedChemExpress
SKU: HY-Y0304
Rating: 5.0 (10 reviews)

Cat. No.: HY-Y0304 Purity: 99.85%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Dibutyl phthalate is a commonly used plasticizer commonly found in some food packaging materials, personal care products, and the coating of oral medications. May cause toxicity and adverse neurobehavioral effects.

For research use only. We do not sell to patients.

CAS No. : 84-74-2

Size	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Liquid
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

or Bulk Inquiry

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of Dibutyl phthalate:

10 Publications Citing Use of MCE Dibutyl phthalate

In Vivo Efficacy Study

IHC

Bio/Physico-chemical Assay

: Dibutyl phthalate purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2026 Apr 1:314:120051. [Abstract]; Mice in the Dibutyl phthalate (DBP, 50-200 mg/kg, 28 days)-treated group displayed lethargy, disheveled coats, and sluggish responses. Adverse reactions intensified with increasing DBP doses, though no mouse deaths occurred during the trial.

: Dibutyl phthalate purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2026 Apr 1:314:120051. [Abstract]; With increasing Dibutyl phthalate (DBP, 50-200 mg/kg, 28 days) doses, mouse body weight gradually decreased while liver weight and organ coefficient progressively increased.

: Dibutyl phthalate purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2026 Apr 1:314:120051. [Abstract]; Histopathological analysis revealed that Dibutyl phthalate (DBP, 50-200 mg/kg, 28 days) exposure caused severe liver damage characterized by disrupted hepatic cord arrangement, hepatocyte swelling, and the appearance of lipid droplets.

: Dibutyl phthalate purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2026 Apr 1:314:120051. [Abstract]; Dibutyl phthalate (DBP) exposure induced differential expression in 454 genes, with 240 up-regulated and 214 down-regulated genes identified.

: Dibutyl phthalate purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2026 Apr 1:314:120051. [Abstract]; Dibutyl phthalate (DBP, 50-200 mg/kg, 28 days) exposure induced severe damage to the intestinal physical barrier. Histological examination showed shortened and disordered ileal villi.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Dibutyl phthalate is a commonly used plasticizer commonly found in some food packaging materials, personal care products, and the coating of oral medications^[1]. May cause toxicity and adverse neurobehavioral effects^[2]^[3].

Cellular Effect

Cell Line	Type	Value	Description	References
B16-F10	IC₅₀	0.23 mM Compound: 1	Inhibition of cathepsin B in mouse B16F10 cells Inhibition of cathepsin B in mouse B16F10 cells	[PMID: 18289850]

In Vitro

Dibutyl phthalate (0.001 μg/mL-1000 μg/mL) is detrimental to follicle growth and viability and results in significant dysregulation of cell cycle and apoptosis gene expression in a dose-specific manner. But MBP does not play a role in Dibutyl phthalate toxicity in follicles exposed in vitro^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Dibutyl phthalate (200, 400, or 600 mg/kg/day) induces decrease mice weight, impairment of spermatogenesis, reduces serum follicle stimulating hormone and testosterone level, alters testicular LDH, increases LPO, and decreases the levels of enzymatic antioxidants with histopathological anomalies^[2].

Dibutyl phthalate (6.25, 12.5, 25, 50, 100 and 200 mg/kg) could cause some neurobehavioral adverse effects in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

278.34

Formula

C₁₆H₂₂O₄

CAS No.

84-74-2

Appearance

Liquid (Density: 1.043 g/cm³ )

Color

Colorless to light yellow

SMILES

O=C(C1=CC=CC=C1C(OCCCC)=O)OCCCC

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (359.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : ≥ 50 mg/mL (179.64 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5927 mL	17.9636 mL	35.9273 mL
5 mM	0.7185 mL	3.5927 mL	7.1855 mL
10 mM	0.3593 mL	1.7964 mL	3.5927 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 4

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 5

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: Corn Oil
Solubility: ≥ 100 mg/mL (359.27 mM); Clear solution

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.85%

Select Batch:

Data Sheet (276 KB) SDS (622 KB)

COA (249 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Rasmussen LM, et al. Effects of in vitro exposure to dibutyl phthalate, mono-butyl phthalate, and acetyl tributyl citrate on ovarian antral follicle growth and viability. Biol Reprod. 2017 May 1;96(5):1105-1117. [Content Brief]

[2]. Aly HA, et al. Dibutyl phthalate induces oxidative stress and impairs spermatogenesis in adult rats. Toxicol Ind Health. 2016 Aug;32(8):1467-1477. [Content Brief]

[3]. Farzanehfar V, et al. Determination of dibutyl phthalate neurobehavioral toxicity in mice. Food Chem Toxicol. 2016 Aug;94:221-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	3.5927 mL	17.9636 mL	35.9273 mL	89.8182 mL
	5 mM	0.7185 mL	3.5927 mL	7.1855 mL	17.9636 mL
	10 mM	0.3593 mL	1.7964 mL	3.5927 mL	8.9818 mL
	15 mM	0.2395 mL	1.1976 mL	2.3952 mL	5.9879 mL
	20 mM	0.1796 mL	0.8982 mL	1.7964 mL	4.4909 mL
	25 mM	0.1437 mL	0.7185 mL	1.4371 mL	3.5927 mL
	30 mM	0.1198 mL	0.5988 mL	1.1976 mL	2.9939 mL
	40 mM	0.0898 mL	0.4491 mL	0.8982 mL	2.2455 mL
	50 mM	0.0719 mL	0.3593 mL	0.7185 mL	1.7964 mL
	60 mM	0.0599 mL	0.2994 mL	0.5988 mL	1.4970 mL
	80 mM	0.0449 mL	0.2245 mL	0.4491 mL	1.1227 mL
	100 mM	0.0359 mL	0.1796 mL	0.3593 mL	0.8982 mL