1. Neuronal Signaling
  2. Amyloid-β
  3. Leucomethylene blue mesylate

Leucomethylene blue mesylate (Synonyms: TRx0237 mesylate; Methylene blue leuco base mesylate)

Cat. No.: HY-19948 Purity: 98.75%
Handling Instructions

Leucomethylene blue (TRx0237) mesylate, an orally active second-generation tau protein aggregation inhibitor (Ki of 0.12 μM), could be used for the study of Alzheimer's Disease. Leucomethylene blue mesylate is a common reduced form of Methylene Blue, Methylene Blue is a member of the thiazine class of dyes.

For research use only. We do not sell to patients.

Leucomethylene blue mesylate Chemical Structure

Leucomethylene blue mesylate Chemical Structure

CAS No. : 1236208-20-0

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Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 66 In-stock
Estimated Time of Arrival: December 31
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5 mg USD 60 In-stock
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10 mg USD 90 In-stock
Estimated Time of Arrival: December 31
50 mg USD 270 In-stock
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100 mg USD 420 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 3 publication(s) in Google Scholar

Top Publications Citing Use of Products

2 Publications Citing Use of MCE Leucomethylene blue mesylate

    Leucomethylene blue mesylate purchased from MCE. Usage Cited in: Bioorg Med Chem. 2018 Sep 1;26(16):4693-4705.

    Imaging of HEK-293T cells incubated with or without LMTM (0.5 μM) and 30 (1 μM).
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Leucomethylene blue (TRx0237) mesylate, an orally active second-generation tau protein aggregation inhibitor (Ki of 0.12 μM), could be used for the study of Alzheimer's Disease. Leucomethylene blue mesylate is a common reduced form of Methylene Blue, Methylene Blue is a member of the thiazine class of dyes[1][2][3].

    IC50 & Target

    Ki: 0.12 μM (tau)[3].

    In Vitro

    Leucomethylene blue (100 nM, 48 h) mesylate not only decreases the tau and p-tau expression levels, but also reversed the promoting effects of Aβ25-35 on orexin A and adenosine A1R expression levels[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Western Blot Analysis[2]

    Cell Line: Human SH-SY5Y cell line.
    Concentration: 100 nM.
    Incubation Time: 48 h.
    Result: Aβ25-35 and TRx 0237 co-treatment significantly reversed the promoting effects of Aβ25-35 on tau, p-tau, orexin A and adenosine A1R expression.
    Molecular Weight

    477.62

    Formula

    C18H27N3O6S3

    CAS No.
    SMILES

    CN(C)C1=CC(SC2=CC(N(C)C)=CC=C2N3)=C3C=C1.CS(=O)(O)=O.CS(=O)(O)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, stored under nitrogen, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 110 mg/mL (230.31 mM; Need ultrasonic)

    H2O : 83.33 mg/mL (174.47 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0937 mL 10.4686 mL 20.9371 mL
    5 mM 0.4187 mL 2.0937 mL 4.1874 mL
    10 mM 0.2094 mL 1.0469 mL 2.0937 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 7.5 mg/mL (15.70 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.75 mg/mL (5.76 mM); Clear solution

    *All of the co-solvents are available by MCE.
    References

    Purity: 98.75%

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    Product Name:
    Leucomethylene blue mesylate
    Cat. No.:
    HY-19948
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