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  3. ML-7 hydrochloride

ML-7 hydrochloride 

Cat. No.: HY-15417 Purity: 99.75%
Handling Instructions

ML-7 hydrochloride is a naphthalene sulphonamide derivative, potently inhibits MLCK (IC50=300 nM).

For research use only. We do not sell to patients.

ML-7 hydrochloride Chemical Structure

ML-7 hydrochloride Chemical Structure

CAS No. : 110448-33-4

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10 mM * 1  mL in DMSO USD 87 In-stock
Estimated Time of Arrival: December 31
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50 mg USD 348 In-stock
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Customer Review

Based on 3 publication(s) in Google Scholar

Top Publications Citing Use of Products

    ML-7 hydrochloride purchased from MCE. Usage Cited in: Toxicol Lett. 2020 Jul 16;332:146-154.

    Cells are incubated with 10 μM ML-7 for 1 h, followed by incubation with 1.0 μM As2O3 for 24 h and assessment of occludin protein expression with Western blotting.
    • Biological Activity

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    • References

    • Customer Review

    Description

    ML-7 hydrochloride is a naphthalene sulphonamide derivative, potently inhibits MLCK (IC50=300 nM).

    IC50 & Target

    IC50: 300 nM (MLCK)[1]

    In Vitro

    ML-7 hydrochloride inhibits rabbit portal vein α1-adrenoceptor NSCC with IC50 of 0.8 μM[1]. The myosin light chain kinase (MLCK) inhibitor ML-7 hydrochloride (3 μ M and 10 μM) also attenuates the Dexmedetomidine (DMT)-induced contraction (p<0.05 versus control)[2].

    In Vivo

    In sham operated animals Evans Blue extravasation is not different between ML-7 hydrochloride and vehicle group (sham+vehicle: 0.26±0.02 OD/g; sham+ML-7: 0.26±0.02 OD/g). After CCI inhibition of MLCK with ML-7 results in a significant lower amount of intracerebral Evans Blue compared to vehicle treated animals (CCI+vehicle: 0.42±0.04 OD/g; CCI+ML-7: 0.35±0.05 OD/g, p=0.048)[3].

    Molecular Weight

    452.74

    Formula

    C₁₅H₁₈ClIN₂O₂S

    CAS No.

    110448-33-4

    SMILES

    O=S(N1CCNCCC1)(C2=C3C=CC=C(I)C3=CC=C2)=O.[H]Cl

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 43 mg/mL (94.98 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2088 mL 11.0439 mL 22.0877 mL
    5 mM 0.4418 mL 2.2088 mL 4.4175 mL
    10 mM 0.2209 mL 1.1044 mL 2.2088 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 1.67 mg/mL (3.69 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 1.67 mg/mL (3.69 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Animal Administration
    [3]

    Mice[3]
    Mice are treated with intraperitoneal injection of the selective MLCK inhibitor ML-7 (1 mg/kg) or vehicle solution (0.9% NaCl, control group) 1 h prior to and 6 h after trauma. Hemispheric brain water content and neurological function are measured 24 h after controlled cortical impact (CCI) in animals randomized to: (i) vehicle+sham surgery, (ii) vehicle+CCI, (iii) ML-7+sham surgery or (iv) ML-7+CCI (n=8 per group) and 15 min after CCI in animals randomized to: (i) vehicle+sham surgery, (ii) vehicle+CCI, (iii) ML-7+sham surgery or (iv) ML-7+CCI (n=6 per group). Mice are treated with intraperitoneal injection of the selective MLCK inhibitor ML-7 (1 mg/kg) or vehicle solution (0.9% NaCl, control group) 1 h prior to and 6 h after trauma. Evans Blue extravasation is determined 24 h after surgery in animals randomized to: (i) vehicle + CCI or (ii) ML-7+CCI (n=5 per group) and in animals randomized to (iii) vehicle + sham surgery or (iv) ML-7+sham surgery (n=6 per group). ICP is measured 24 h CCI and sham surgery.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 99.75%

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    Keywords:

    ML-7ML7ML 7MyosinInhibitorinhibitorinhibit

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