Fluorescent Dye
Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.
Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.
Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.
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Fluorescent Dye Related Products (4889)
Related Products (4889)
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Antibodies (4)
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5MP-Propargyl
0 ImagesCat. No.: HY-D1404CAS No.: 2100286-15-35MP-Propargyl is the derivative of 5-Methylene pyrrolone (5MP). 5-Methylene pyrrolones (5MPs) are thiol-specific, reversible bioconjugation reagents for cysteine-specific protein modification, that are commonly used for protein bioconjugation. 5MP-Propargyl is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
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Endo-pH
0 ImagesCat. No.: HY-D3163Endo-pH is a cell-permeable, pH-responsive fluorescent probe based on BODIPY, with a pKa of 5.08. When pH decreases from 7.32 to 4.97, Endo-pH undergoes protonation under acidic conditions, resulting in a 79-fold increase in fluorescence intensity at 786 nm. The detection wavelengths of its protonated form are Ex/Em=688/786 nm, while the absorption wavelength of its non-protonated form is 745 nm. Endo-pH can be used for staining late endosomes in live cells and determining the fusion time of late endosomes and lysosomes.
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NtHzBtd
0 ImagesCat. No.: HY-D3125NtHzBtd is a fluorescent probe for detecting Fe3+. NtHzBtd is applicable to the selective fluorescent detection of Fe3+ ions and live cell imaging studies. NtHzBtd can selectively coordinate with Fe3+ to form a 1:1 complex, triggering chelation enhanced quenching (CHEQ) and intramolecular charge transfer (ICT) processes, which result in fluorescence turn-off, thereby enabling sensitive detection of Fe3+ and live cell fluorescence imaging. After binding to Fe3+, NtHzBtd reduces fluorescence intensity, exhibits a rapid response property, with a limit of detection of 0.036 μM and a response time of approximately 55 s. The detection wavelengths are Ex/Em = 334/401 nm (solution system).
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Pyrromethene 556 (solution)
0 ImagesCat. No.: HY-DY1122CAS No.: 121461-69-6Pyrromethene 556 (solution) is a fluorescent dipyrromethene-BF2 complex laser dye. Pyrromethene 556 (solution) enables immediate and reversible color changes in conventional glass ionomer restorative materials, while it does not cause persistent, visually detectable color changes in other restorative materials.
Solvent and concentration: DMSO: 10 mM
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Cy3 labled Mongersen sodium
0 ImagesCat. No.: HY-145721ECy3 labled Mongersen sodium is a Cy3 labled Mongersen sodium.
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FAM labled Elebsiran sodium
0 ImagesCat. No.: HY-147266DFAM labled Elebsiran sodiumis a FAM labled Elebsiran sodium.
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DNA intercalator 2
0 ImagesCat. No.: HY-D1101CAS No.: 864281-69-6DNA intercalator 2 is a DNA intercalating agent that can be used for DNA staining.
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Lyso Flipper-TR 29
0 ImagesCat. No.: HY-D2321CAS No.: 2324152-35-2Lyso Flipper-TR 29 is a Flipper probe that can label lysosomes. Lyso Flipper-TR 29 enters lysosomes and late endosomes by transient deprotonation to cross their membranes in neutral form.
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RB-PEG10000-Biotin
0 ImagesCat. No.: HY-D2889HSynonyms: Rhodamine B-PEG10000-BiotinRB-PEG10000-Biotin (Rhodamine B-PEG10000-Biotin) is a fluorescent dye composed of Rhodamine B (HY-Y0016), PEG and a Biotin. Biotin can bind with high affinity to streptavidin or avidin. RB-PEG10000-Biotin plays an important role in the specific capture and detection of biomolecules (Ex/Em=546/610 nm).
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FAM labled Vutrisiran sodium
0 ImagesCat. No.: HY-132589DFAM labled Vutrisiran sodiumis a FAM labled Vutrisiran sodium.
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BCECF (solution)
0 ImagesCat. No.: HY-DY1098CAS No.: 85138-49-4Synonyms: 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein (solution)BCECF (solution) (2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein (solution)) is a cell-impermeant pH-sensitive fluorescent dye. BCECF allows measurements in the physiological pH range 6.0-8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm.
Solvent and concentration: DMSO: 10 mM
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CEMT
0 ImagesCat. No.: HY-D3166CAS No.: 2413816-64-3CEMT is a carboxylesterase (CES) substrate and a ratiometric two-photon fluorescent reporter probe. CEMT can be hydrolyzed by CES to generate HMT, which is used for mitochondrial pH sensing. After activation by CES, CEMT exhibits ratiometric fluorescence changes in response to pH variations. CEMT targets and covalently binds to mitochondria, and can avoid leakage during acidification, thus enabling in situ imaging (Ex/Em = 410 nm/550 nm).
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Cy3 labled Elebsiran sodium
0 ImagesCat. No.: HY-147266ECy3 labled Elebsiran sodium is a Cy3 labled Elebsiran sodium.
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RB-PEG5000-NHS
0 ImagesCat. No.: HY-D2884ESynonyms: Rhodamine B-PEG5000-NHSRB-PEG5000-NHS (Rhodamine B-PEG5000-NHS) is a fluorescent dye composed of Rhodamine B (HY-Y0016), PEG and a hydroxysuccinimide (NHS) group. The NHS group can react with amine-containing groups. RB-PEG5000-NHS can be used for fluorescent labeling and imaging (Ex/Em=546/610 nm).
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6-FAM cyclohexyl phosphoramidite
0 ImagesCat. No.: HY-D3486CAS No.: 952290-59-46-FAM cyclohexyl phosphoramidite is a derivative of 6-carboxyfluorescein (6-FAM) featuring a phosphoramidite moiety, used in oligonucleotide synthesis. It is primarily employed in the synthesis of fluorescent oligonucleotide probes (Ex/Em = 495/519 nm).
