Cat. No.: HY-75161

(-)-Menthol

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

(-)-Menthol is a key component of peppermint oil that binds and activates transient receptor potential melastatin 8 (TRPM8), a Ca²⁺-permeable nonselective cation channel, to increase [Ca²⁺]_i. Antitumor activity.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 2216-51-5
Formula: C₁₀H₂₀O
Molecular Weight:156.27

Storage:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

(-)-Menthol Chemical Structure

CAS No.: 2216-51-5

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Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

TRPM8^[1]

All TRP Channel Isoforms :

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	56 μM Compound: 27	Antagonist activity at mouse TRPA1 channel expressed in CHO cells assessed as blockade of agonist-induced whole cell conductance by electrophysiology Antagonist activity at mouse TRPA1 channel expressed in CHO cells assessed as blockade of agonist-induced whole cell conductance by electrophysiology	[PMID: 20356305]
CHO	EC₅₀	95 μM Compound: Menthol	Agonist activity at mouse TRPA1 expressed in CHO cells assessed as increase in calcium influx by patch clamp electrophysiology method Agonist activity at mouse TRPA1 expressed in CHO cells assessed as increase in calcium influx by patch clamp electrophysiology method	[PMID: 30878828]
HEK293	EC₅₀	10.1 μM Compound: L-Menthol	Agonist activity at full length recombinant human TRPM8 expressed in HEK293 cells assessed as increase in intracellular Ca2+ level pre-incubated for 5 mins by Fluo4-AM dye-based fluorescence assay Agonist activity at full length recombinant human TRPM8 expressed in HEK293 cells assessed as increase in intracellular Ca2+ level pre-incubated for 5 mins by Fluo4-AM dye-based fluorescence assay	[PMID: 28740604]
HEK293	EC₅₀	11.7 μM Compound: Menthol	Activity at human TRPM8 K856A mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 K856A mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	29 μM Compound: Menthol	Activity at human TRPM8 expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	3 μM Compound: Menthol	Inhibition of human TRPM8 (unknown origin) expressed in HEK293 cells assessed as inhibition of WS12-induced Ca2+ elevation by FLIPR assay Inhibition of human TRPM8 (unknown origin) expressed in HEK293 cells assessed as inhibition of WS12-induced Ca2+ elevation by FLIPR assay	[PMID: 24080460]
HEK293	EC₅₀	30 μM Compound: Menthol	Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay	[PMID: 30878828]
HEK293	EC₅₀	34.1 μM Compound: Menthol	Activity at human TRPM8 H845A mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 H845A mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	35.3 μM Compound: Menthol	Activity at human TRPM8 K856R mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 K856R mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	38.4 μM Compound: Menthol	Activity at human TRPM8 R851Q mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 R851Q mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	38.8 μM Compound: Menthol	Activity at human TRPM8 R862A mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 R862A mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	477 μM Compound: Menthol	Activity at human TRPM8 S4b_M mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 S4b_M mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	75 μM Compound: Menthol	Agonist activity at rat TRPM8 expressed in HEK293 cells assessed as increase in current amplitude at +80 mV holding potential by whole cell patch-clamp assay Agonist activity at rat TRPM8 expressed in HEK293 cells assessed as increase in current amplitude at +80 mV holding potential by whole cell patch-clamp assay	[PMID: 26847872]
HEK293	EC₅₀	818 μM Compound: Menthol	Activity at human TRPM8 R842A mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 R842A mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	93 μM Compound: Menthol	Activity at human TRPM8 R842K mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation Activity at human TRPM8 R842K mutant expressed in HEK293 cells assessed as compound concentration causing leftward shift of voltage for half maximal activation	[PMID: 17293875]
HEK293	EC₅₀	96 μM Compound: L-Menthol	Agonist activity at recombinant human TRPA1 expressed in HEK293 cells assessed as increase in intracellular Ca2+ level pre-incubated for 5 mins by Fluo4-AM dye-based fluorescence assay Agonist activity at recombinant human TRPA1 expressed in HEK293 cells assessed as increase in intracellular Ca2+ level pre-incubated for 5 mins by Fluo4-AM dye-based fluorescence assay	[PMID: 28740604]
HEK293	IC₅₀	19 μM Compound: Menthol	Displacement of [3H]menthol from human TRPM8 K856A mutant expressed in HEK293 cell membranes by scintillation counting Displacement of [3H]menthol from human TRPM8 K856A mutant expressed in HEK293 cell membranes by scintillation counting	[PMID: 17293875]
HEK293	IC₅₀	520 μM Compound: Menthol	Displacement of [3H]menthol from human TRPM8 R842A mutant expressed in HEK293 cell membranes by scintillation counting Displacement of [3H]menthol from human TRPM8 R842A mutant expressed in HEK293 cell membranes by scintillation counting	[PMID: 17293875]
HEK293	IC₅₀	92 μM Compound: Menthol	Displacement of [3H]menthol from human TRPM8 expressed in HEK293 cell membranes by scintillation counting Displacement of [3H]menthol from human TRPM8 expressed in HEK293 cell membranes by scintillation counting	[PMID: 17293875]

In Vitro

(-)-Menthol (Menthol ) per se does not exhibit antiproliferative activity, but it is able to enhance 1α,25(OH)₂D₃-mediated growth inhibition in LNCaP cells. At high (-)-Menthol concentrations above 1.6 mM, the cells begin to detach from the culture dish^[1].
(-)-Menthol (0.8 mM) can increase [Ca²⁺]i via transmembrane influx or store release pathways. Peak increase in [Ca²⁺]_i was 102.3±39 nM (n=3) in (-)-Menthol alone and 124.5±51 nM (n=3) in combination of 1α,25(OH)₂D₃ with (-)-Menthol, respectively^[1].
Combination of 1α,25(OH)₂D₃ with (-)-Menthol (0.8 mM) cooperatively modulates bcl-2 and p21 expression^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 2216-51-5
Appearance <41°C Solid,>44°C Liquid
Molecular Weight 156.27
Formula C₁₀H₂₀O
Color Colorless to off-white
SMILES

O[C@H]1[C@H](C(C)C)CC[C@@H](C)C1
Structure Classification
- Terpenoids
- Other Monoterpenes
Initial Source
- Plants
- Labiatae
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Publications (4)

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Journal Impact Factor

Nat Commun

Noncanonical calcium-independent TRPM4 activation governs intestinal fluid homeostasis. [Abstract]

2026 Jan 8;17(1):1253. PMID: 41507162
Chem Eng J

2026 Feb 5.
Invest Ophthalmol Vis Sci

Effect of TRPM8 Functional Loss on Corneal Epithelial Wound Healing in Mice. [Abstract]

2023 Jan 3;64(1):19. PMID: 36692471

(-)-Menthol purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2023 Jan 3;64(1):19. [Abstract]

Topical administration of the TRPM8 agonist (-)-menthol (200 µM; four times per day for 2 d) to eyes of C57BL/6J mice after debridement delayed corneal epithelial healing.
Cells Dev

Small molecules induces dorsal root ganglion satellite glial cells to differentiate into sensory neuron-like cells. [Abstract]

2026 Mar:185:204073. PMID: 41690597

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (639.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1.89 mg/mL (12.09 mM; ultrasonic and warming and heat to 60°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

6.3992 mL

31.9959 mL

63.9918 mL

5 mM

1.2798 mL

6.3992 mL

12.7984 mL

10 mM

0.6399 mL

3.1996 mL

6.3992 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (16.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (16.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (16.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Park EJ, et al. Menthol Enhances an Antiproliferative Activity of 1alpha,25-Dihydroxyvitamin D(3) in LNCaP Cells. J Clin Biochem Nutr. 2009 Mar;44(2):125-30. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	6.3992 mL	31.9959 mL	63.9918 mL	159.9795 mL
	5 mM	1.2798 mL	6.3992 mL	12.7984 mL	31.9959 mL
	10 mM	0.6399 mL	3.1996 mL	6.3992 mL	15.9980 mL
DMSO	15 mM	0.4266 mL	2.1331 mL	4.2661 mL	10.6653 mL
	20 mM	0.3200 mL	1.5998 mL	3.1996 mL	7.9990 mL
	25 mM	0.2560 mL	1.2798 mL	2.5597 mL	6.3992 mL
	30 mM	0.2133 mL	1.0665 mL	2.1331 mL	5.3327 mL
	40 mM	0.1600 mL	0.7999 mL	1.5998 mL	3.9995 mL
	50 mM	0.1280 mL	0.6399 mL	1.2798 mL	3.1996 mL
	60 mM	0.1067 mL	0.5333 mL	1.0665 mL	2.6663 mL
	80 mM	0.0800 mL	0.3999 mL	0.7999 mL	1.9997 mL
	100 mM	0.0640 mL	0.3200 mL	0.6399 mL	1.5998 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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(-)-Menthol

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Publications Citing Use of MedChemExpress (MCE) (-)-Menthol

All TRP Channel Isoforms

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

(-)-Menthol Related Classifications

Cat. No.: HY-75161