Anti-CD3 Antibody (OKT-3)
Based on 1 Customer Validation
Anti-CD3 Antibody (OKT-3) is a kind of mouse IgG2a κ antibody inhibitor, targeting to human CD3. Anti-CD3 Antibody (OKT-3) decreases T cells. Anti-CD3 Antibody (OKT-3) can be used for the research of immunology, such as graft-versus-host disease.
For research use only. We do not sell to patients.
- Purity: 97.94%
- Molecular Weight:146.34 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Mouse IgG2a kappa
Human
CD3
Anti-CD3 Antibody (OKT-3) (1 μg/mL, 24-36 h) decreases CD4+ and CD8+ T cell numbers in huCD3εHET cells[1].
Anti-CD3 Antibody (OKT-3) (1-1000 ng/mL, 2 h) leads to the marked proliferation in human peripheral blood mononuclear cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Anti-CD3 Antibody (OKT-3) (10 mg/kg, i.p., a single dose) prevents graft-versus-host disease in human peripheral blood/umbilical cord blood-induced graft-versus-host disease mice models[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:NOG mice were injected with PBMCs from a healthy donor[2]
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Dosage:20 μg/mouse
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Administration:Intravenouslyly injection
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Result:Increased IL-2, IL6, TNF-α and IF-γ levels.
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Animal Model:Human peripheral blood/umbilical cord blood-induced graft-versus-host disease mice models[3]
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Dosage:10 mg/kg
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Administration:Intravenouslyly injection
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Result:Exhibited nearly complete elimination of CD451CD31 cells in PB.
Decreased T cells in spleens.
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
ELISA, FACS, Functional assay, Research in vivo
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The Anti-CD3 Antibody (OKT-3) binds to huCD3e-Jurkat cells.
Chemical Information
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Appearance Liquid
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Molecular Weight 146.34 kDa
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Color Colorless to light yellow
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SMILES
[Anti-CD3 Antibody (OKT-3)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Crespo J, et al. A humanized CD3ε-knock-in mouse model for pre-clinical testing of anti-human CD3 therapy. PLoS One. 2021 Feb 17;16(2):e0245917. [Content Brief]
[2]. Shiheido H, et al. Modulation of the human T cell response by a novel non-mitogenic anti-CD3 antibody. PLoS One. 2014 Apr 7;9(4):e94324. [Content Brief]
[3]. Wunderlich M, et al. OKT3 prevents xenogeneic GVHD and allows reliable xenograft initiation from unfractionated human hematopoietic tissues. Blood. 2014 Jun 12;123(24):e134-44. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-CD3 Antibody (OKT-3) Related Classifications
HY-P990864 Related Classifications
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