Anti-CD28 Antibody (9.3)
Based on 1 Customer Validation
Anti-CD28 Antibody (9.3) is an anti-human CD28 IgG2a monoclonal antibody. Anti-CD28 Antibody (9.3) can activate T cells. Anti-CD28 Antibody (9.3) can be used for research on immunology. The recommend isotype control of Anti-CD28 Antibody (9.3): Mouse IgG2a kappa, Isotype Control (HY-P99978).
For research use only. We do not sell to patients.
- Purity: 99%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Mouse IgG2a
Human
CD28
Anti-CD28 Antibody (9.3) (2 μg/mL, 48 h) can activate and enhance T cell response combined with CD3 mAb (145-2C11) (HY-P990802)[1].
Anti-CD28 Antibody (9.3) (2-5 h) promotes T cell activation combined with CD3 mAb (OKT-3) (HY-P990864)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
in vitro T cell stimulation/activation
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Flow cytometric analysis of 1X106 Juakat cells with Anti-CD28 Antibody (HY-P990865, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG1 kappa (HY-P99977, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-CD28 Antibody (9.3)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (257 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Huang H, et al. EGFR mutations induce the suppression of CD8+ T cell and anti-PD-1 resistance via ERK1/2-p90RSK-TGF-β axis in non-small cell lung cancer. J Transl Med. 2024 Jul 14;22(1):653. [Content Brief]
[2]. Zhou X, et al. IL-6 drives T cell death to participate in lymphopenia in COVID-19. Int Immunopharmacol. 2022 Oct;111:109132. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)