Anti-Mouse ICOSL/CD275 Antibody (HK5.3)
Based on 1 Customer Validation
Anti-Mouse ICOSL/CD275 Antibody (HK5.3) is an anti-mouse ICOSL/CD275 IgG2a monoclonal antibody. Anti-Mouse ICOSL/CD275 Antibody (HK5.3) can inhibit the adhesion between T cells and endothelial cells. Anti-Mouse ICOSL/CD275 Antibody (HK5.3) can reduce the expansion of tissue resident (TR) Treg cells. Anti-Mouse ICOSL/CD275 Antibody (HK5.3) can be used for researches on inflammation and infection conditions such as influenza virus infection and arthritis.
For research use only. We do not sell to patients.
- Purity : 98.59%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Rat IgG2a kappa
Recommend Isotype Controls
Species Reactivity
Mouse
IC50 & Target
ICOSL/CD275
In Vitro
Anti-Mouse ICOSL/CD275 Antibody (HK5.3) (15 μg/mL, 30 min) significantly reduces adhesion of Th1 and Th17 cells to primary mouse brain microvascular endothelial cells (pMBMECs)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Anti-Mouse ICOSL/CD275 Antibody (HK5.3) (250-500 μg, i.v., on day -1, 1 and 3) significantly reduces the expansion of TR Treg cells in C57BL/6 mice with an influenza virus infection model[3].
Anti-Mouse ICOSL/CD275 Antibody (HK5.3) (15 mg/kg, i.p., on day 19, 21, 23, and 25) significantly delays the onset of arthritis in female DBA1 mice with a bovine collagen-induced arthritis model[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:HDTV injected wild-type C57Bl/6 mice[1]
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Dosage:100 μg
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Administration:Intraperitoneal injection (i.p.), on day 0, 3, 6, and 9 after HDTV injection
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Result:Significantly increased the number of liver cells.
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Animal Model:200 PFU H1N1 virus primary infected and 2×104H3N2 virus followed infected C57BL/6 mice (6-15 weeks)[3]
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Dosage:500 μg for first-dose, 250 μg for subsequent doses
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Administration:Intravenous injection (i.v.), on day -1, 1 and 3
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Result:Significantly reduced the expansion of TR Treg cells (especially IL-10+ subpopulation), but did not affect other T cell subpopulations.
Had no significant effect on PD-1 expression, but reduced the survival rate of TR Treg cells.
Reduced the level of IL-10.
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Animal Model:Bovine collagen injected female DBA1 mice[4]
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Dosage:15 mg/kg
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Administration:Intraperitoneal injection (i.p.), on day 19, 21, 23, and 25
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Result:Significantly delayed the onset of arthritis.
Reduced inflammation score.
Reduced joint damage, cartilage injury, and inflammatory cell infiltration.
Gene ID
Accession
Q9JHJ8-1
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
in vivo ICOSL neutralization
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse ICOSL/CD275 Antibody (HK5.3)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Yin K, et al. Senescence-induced endothelial phenotypes underpin immune-mediated senescence surveillance. Genes Dev. 2022 May 1;36(9-10):533-549. [Content Brief]
[2]. Johann L, et al. A20 regulates lymphocyte adhesion in murine neuroinflammation by restricting endothelial ICOSL expression in the CNS. J Clin Invest. 2023 Dec 15;133(24):e168314. [Content Brief]
[3]. McGee MC, et al. PD-1 and ICOS counter-regulate tissue resident regulatory T cell development and IL-10 production during flu. Front Immunol. 2022 Sep 8;13:984476. [Content Brief]
[4]. O'Dwyer R, et al. Anti-ICOSL New Antigen Receptor Domains Inhibit T Cell Proliferation and Reduce the Development of Inflammation in the Collagen-Induced Mouse Model of Rheumatoid Arthritis. J Immunol Res. 2018 Oct 17;2018:4089459. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse ICOSL/CD275 Antibody (HK5.3) Related Classifications
HY-P990123 Related Classifications
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