Anti-Mouse L-Selectin/CD62L Antibody (Mel-14)
Based on 1 Customer Validation
Anti-Mouse L-Selectin/CD62L Antibody (Mel-14) is an anti-mouse L-Selectin/CD62L IgG2a monoclonal antibody. Anti-Mouse L-Selectin/CD62L Antibody (Mel-14) can significantly inhibit the migration of T cells and B cells. Anti-Mouse L-Selectin/CD62L Antibody (Mel-14) can block initial lymphocyte homing. Anti-Mouse L-Selectin/CD62L Antibody (Mel-14) can be used for researches on inflammation, metabolic and infection conditions such as diabetes and parasitic infections.
For research use only. We do not sell to patients.
- Purity : 95.00%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Rat IgG2a kappa
Recommend Isotype Controls
Species Reactivity
Mouse
IC50 & Target
L-Selectin/CD62L
In Vivo
Anti-Mouse L-Selectin/CD62L Antibody (Mel-14) (250 μg, i.p., single dose) effectively reduces the number of precursors (preDCs) in wild type CD57BL/6 mice[2].
Anti-Mouse L-Selectin/CD62L Antibody (Mel-14) (100 μg, i.p., twice a week, from 3 to 8 weeks or from 1 to 10 weeks) significantly reduce the incidence of diabetes in NOD-Nk mice with a diabetes model[3].
Anti-Mouse L-Selectin/CD62L Antibody (Mel-14) (200 μg, i.p., single dose) inhibits parasitic infections in BALB/c mice with a Leishmania major infection model[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:LDBM and LPS (HY-D1056) induced CD57BL/6 mice (8-10 weeks)[1]
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Dosage:200 μg
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Administration:Intraperitoneal injection (i.p.), single dose
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Result:Significantly inhibited the migration of B lymphocytes to regional lymph nodes, but did not affect the migration of granulocyte-macrophage progenitor (GMP) to regional lymph nodes.
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Animal Model:Wild type CD57BL/6 mice (6-30 weeks) injected with preDCs[2]
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Dosage:250 μg
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Administration:Intraperitoneal injection (i.p.), single dose
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Result:Reduced the number of preDCs in lymph nodes.
Had a significant inhibitory effect on T cell migration.
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Animal Model:NOD-Nk mice (1-10 weeks)[3]
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Dosage:100 μg
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Administration:Intraperitoneal injection (i.p.), from 3 to 8 weeks or from 1 to 10 weeks
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Result:Significantly reduced the incidence of insulitis and diabetes.
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Animal Model:2×106 Leishmania major injected BALB/c mice[4]
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Dosage:200 μg
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Administration:Intraperitoneal injection (i.p.), single dose
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Result:Caused mild swelling and healing of the foot pad, while the control group experienced severe foot pad swelling.
Reduced parasites in the spleen and lymph node (LN) by 100-10000 times.
Increased the level of IFN-γ and reduced the level of IL-4 in the spleen and LN.
Gived mice long-lasting resistance to secondary infections
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
in vivo CD62L neutralization
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse L-Selectin/CD62L Antibody (Mel-14)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Serrano-Lopez J, et al. Inflammation rapidly recruits mammalian GMP and MDP from bone marrow into regional lymphatics. Elife. 2021 Apr 8;10:e66190. [Content Brief]
[2]. Ugur M, et al. Lymph node medulla regulates the spatiotemporal unfolding of resident dendritic cell networks. Immunity. 2023 Aug 8;56(8):1778-1793.e10. [Content Brief]
[3]. Lepault F, et al. Lack of L-selectin expression by cells transferring diabetes in NOD mice: insights into the mechanisms involved in diabetes prevention by Mel-14 antibody treatment. Eur J Immunol. 1995 Jun;25(6):1502-7. [Content Brief]
[4]. Laskay T, et al. In vivo blocking of L-selectin rescues BALB/c mice from fatal Leishmania major infection. Immunol Lett. 1997 Jun 1;57(1-3):89-91. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse L-Selectin/CD62L Antibody (Mel-14) Related Classifications
HY-P990201 Related Classifications
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