ASF1A Antibody (YA4584)(PBS only)

Cat. No.: HY-P84887A: Data Sheet User Guide for Antibodies
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ASF1A Antibody (YA4584) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ASF1A.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ASF1A Antibody (YA4584) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ASF1A.

Host

Mouse

Molecular Weight

Predicted band size: 23 kDa;

Observed band size: 23 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9Y294

Gene ID

25842 [NCBI]

Immunogen

Purified recombinant fragment of human ASF1A (AA: 1-204) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis

Subcellular Localization：Cytoplasm

Isoforms & Post-Translational Modification：Q9H0Y0 has 2 isomers: Q9H0Y0-1: 25279 Da (predicted); Q9H0Y0-2: 14217 Da (predicted).

Subunit：Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7 (By similarity). Interacts with IRGM

Synonyms

CIA; CGI-98; HSPC146

Documentation

User Guide for Antibodies (1077 KB)