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CD248 Antibody (YA4508)

(Synonyms: TEM1; CD164L1)
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Based on 1 Customer Validation

CD248 Antibody (YA4508) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD248.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD248 Antibody (YA4508)
  • Experimental Validation Results for CD248 Antibody (YA4508)
  • CD248 Antibody (YA4508) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:400 1:10000

Product Details

Description

CD248 Antibody (YA4508) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD248.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 160 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 80.9 kDa
Immunogen

Purified recombinant fragment of human CD248 (AA: extra 18-180) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD248 Antibody (YA4508)
    Western blot analysis of extracts from HEK293(lane 1(40μg) ),A431(lane 2(40μg) ),A549(lane 3(40μg) )and HepG2(lane 4(40μg) ) using CD248 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH， 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD248 Antibody (YA4508)
    Flow cytometric analysis of 1X10^6 HL-60 cells labeling CD248 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    CD248 cell surface glycoprotein involved in various biological processes including angiogenesis, immune response modulation, and tissue remodeling and repair. Participates in pericyte proliferation through positive modulation of the PDGF receptor signaling pathway. Acts as a scaffold for factor X, triggering allosteric changes and the spatial re-alignment of factor X with the TF-factor VIIa complex, thereby enhancing coagulation activation. Modulates the insulin signaling pathway by interacting with insulin receptor/INSR and by diminishing its capacity to be autophosphorylated in response to insulin. Also regulates LPS-induced inflammatory response in macrophages by favoring the production of proinflammatory cytokines. In human, negatively regulates T-cell proliferation compared with stromal cells where it increases proliferation[1][2].

  • Subcellular Localization

    Membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts. Strongly expressed in pericytes (PubMed:20484976) . Expressed on stromal cells and cells with lymphoid morphology such a T-cells (PubMed:21466550)

  • Isoforms & Post-Translational Modification

    Q9HCU0 has 2 isomers: Q9HCU0-1: 80859 Da (predicted); Q9HCU0-2: 46241 Da (predicted).
    O-glycosylated with sialylated oligosaccharides;May be N-glycosylated

  • Subunit

    Interacts with PDGFRA; this interaction promotes PDGF receptor signaling pathway. Interacts with integrin beta-1/ITGB1.

  • SwissProt ID

    Q9HCU0

  • Gene ID
    57124 [NCBI]
  • Synonyms

    TEM1; CD164L1

CD248 Antibody (YA4508) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293(lane 1(40μg) ),A431(lane 2(40μg) ),A549(lane 3(40μg) )and HepG2(lane 4(40μg) ) using CD248 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH， 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HL-60 cells labeling CD248 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD248 Antibody (YA4508) Product Image
    1/16
  • Experimental Validation Results for CD248 Antibody (YA4508)
  • Experimental Validation Results for CD248 Antibody (YA4508)
  • CD248 Antibody (YA4508) Product Image
  • Experimental Validation Results for CD248 Antibody (YA4508)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293(lane 1(40μg) ),A431(lane 2(40μg) ),A549(lane 3(40μg) )and HepG2(lane 4(40μg) ) using CD248 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH， 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD248 Antibody (YA4508)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HL-60 cells labeling CD248 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD248 Antibody (YA4508)
  • Experimental Validation Results for CD248 Antibody (YA4508)
  • CD248 Antibody (YA4508) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293(lane 1(40μg) ),A431(lane 2(40μg) ),A549(lane 3(40μg) )and HepG2(lane 4(40μg) ) using CD248 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH， 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • CD248 Antibody (YA4508) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 HL-60 cells labeling CD248 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD248 Antibody (YA4508) Product Image WB
  • CD248 Antibody (YA4508) Product Image FC