CD369 Antibody (YA4466)(PBS only)

Cat. No.: HY-P84769A: Data Sheet User Guide for Antibodies
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CD369 Antibody (YA4466) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD369.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CD369 Antibody (YA4466) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD369.

Host

Mouse

Molecular Weight

Predicted band size: 28 kDa;

Observed band size: 28 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9BXN2

Gene ID

64581 [NCBI]

Immunogen

Purified recombinant fragment of human CD369 (AA: extra 66-247) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Lectin that functions as a pattern recognizing receptor (PRR) specific for beta-1,3-linked and beta-1,6-linked glucans, which constitute cell wall constituents from pathogenic bacteria and fungi (PubMed:11567029, PubMed:12423684). Necessary for the TLR2-mediated inflammatory response and activation of NF-kappa-B: upon beta-glucan binding, recruits SYK via its ITAM motif and promotes a signaling cascade that activates some CARD domain-BCL10-MALT1 (CBM) signalosomes, leading to the activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (By similarity). Enhances cytokine production in macrophages and dendritic cells (By similarity). Mediates production of reactive oxygen species in the cell (By similarity). Mediates phagocytosis of C.albicans conidia (PubMed:17230442). Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Induces phosphorylation of SCIMP after binding beta-glucans (By similarity)

Subcellular Localization：Cell membrane; Single-pass type II membrane protein; Cytoplasm; Cytoplasm; Cytoplasm

Expression：
Tissue_specificity:Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta

Induction:Up-regulated during differentiation from monocytes into dendritic cells

Isoforms & Post-Translational Modification：Q9BXN2 has 10 isomers: Q9BXN2-1: 27627 Da (predicted); Q9BXN2-2: 22563 Da (predicted); Q9BXN2-3: 21065 Da (predicted); Q9BXN2-4: 21130 Da (predicted); Q9BXN2-5: 19218 Da (predicted); Q9BXN2-6: 14787 Da (predicted); Q9BXN2-7: 16000 Da (predicted); Q9BXN2-8: 5164 Da (predicted); Q9BXN2-9: 8363 Da (predicted); Q9BXN2-10: 13138 Da (predicted).
Phosphorylated on tyrosine residues in response to beta-glucan binding

Subunit：Homodimer. Interacts with SYK; participates in leukocyte activation in presence of fungal pathogens. Interacts with CD37; this interaction controls CLEC7A-mediated IL-6 production (PubMed:17182550)

Synonyms

CLEC7A; BGR; CANDF4; SCARE2; DECTIN1; CLECSF12

Documentation

User Guide for Antibodies (1077 KB)