CD86 Antibody (YA6374)

Cat. No.: HY-P86682: Data Sheet User Guide for Antibodies
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CD86 Antibody (YA6374) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD86.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CD86 Antibody (YA6374) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD86.

Host

Rabbit

Clonality

Monoclonal

Molecular Weight

Predicted band size: 37 kDa

Species Reactivity

Human

SwissProt ID

P42081

Gene ID

942 [NCBI]

Immunogen

Recombinant human CD86 protein aa 24-239/329

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1ug:Test
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed:12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity)|Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation|(Microbial infection) Acts as a receptor for adenovirus subgroup B

Subcellular Localization：Cell membrane

Expression：
Tissue_Specificity: Expressed by activated B-lymphocytes and monocytes

Isoforms & Post-Translational Modification：P42081 has 6 isomers: P42081-1: 37682 Da (predicted); P42081-3: 37007 Da (predicted); P42081-2: 12818 Da (predicted); P42081-4: 31293 Da (predicted); P42081-5: 24773 Da (predicted); P42081-6: 28420 Da (predicted).
Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation

Subunit：Homodimer

Synonyms

CD28LG2; FUN1; LAB72; Activation B7 2 antigen; Activation B7-2 antigen 3; CD86_HUMAN; Activation B7-2 antigen; Activation B72 antigen; B lymphocyte activation antigen B7 2; B lymphocyte activation antigen B72; B-lymphocyte activation antigen B7-2 2; B-lymphocyte activation antigen B7-2; B7 2; B7 2 antigen; B7; B7-2; B7.2; B70; B72 antigen; BU63; CD 86; CD28 antigen ligand 2 2; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86 antigen(CD28 antigen ligand 2, B7-2 antigen) 1, 2; CD86 antigen; CD86 molecule; CLS1; CTLA 4 counter receptor B7 2; CTLA 4 counter receptor B7.2; CTLA-4 counter-receptor B7.2 2, 3; CTLA4 counter receptor B72; Early T-cell costimulatory molecule 1; ETC-1; FUN 1; LAB72; Ly-58; Ly58; MB7; MB7-2; Membrane glycoprotein; MGC34413; T lymphocyte activation antigen CD86; T-lymphocyte activation antigen CD86; TS/A-2.

Documentation

User Guide for Antibodies (1077 KB)