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CX3CR1 Antibody (YA4051)

(Synonyms: V28; CCRL1; GPR13; CMKDR1; GPRV28; CMKBRL1)
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Based on 1 Customer Validation

CX3CR1 Antibody (YA4051) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CX3CR1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CX3CR1 Antibody (YA4051)
  • Experimental Validation Results for CX3CR1 Antibody (YA4051)
  • CX3CR1 Antibody (YA4051) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

CX3CR1 Antibody (YA4051) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CX3CR1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 40 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 40 kDa
Immunogen

Purified recombinant fragment of human CX3CR1 (AA: extra mix) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CX3CR1 Antibody (YA4051)
    Flow cytometric analysis of 1X10^6 HL-60 cells labeling CX3CR1 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CX3CR1 Antibody (YA4051)
    Immunofluorescence analysis of Hela cells labeling CX3CR1 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CX3CR1 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L (green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

Background

  • Function

    CX3C chemokine receptor1 (CX3CR1), also known as the fractalkine receptor or GPR13, is the specific receptor to the CX3CL1. As the name suggests, CX3CR1 binds the chemokine CX3CL1 (also called fractalkine (in humans) or neurotactin (in mouse) ) . CX3CL1 is a member of the CX3C family and that has been detected on activated endothelial cells, smooth muscle cells (SMCs), macrophages and platelets. CX3CL1 is the only neuronal chemokine in the body with pro-inflammatory, anti-inflammatory and neuroprotective effects, and is involved in the development of numerous inflammatory pathologies including atherosclerosis, rheumatoid arthritis, or graft rejection.

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Expressed in lymphoid and neural tissues (PubMed:7590284) . Expressed in lymphocyte subsets, such as natural killer (NK) cells, gamma-delta T-cells and terminally differentiated CD8 (+) T-cells (PubMed:12055230) . Expressed in smooth muscle cells in atherosclerotic plaques (PubMed:14581400)

    Induction:Expression is down-regulated by miR-27a-5p microRNA in natural killer (NK) cell lymphocytes in response to TGF-beta1

  • Isoforms & Post-Translational Modification

    P49238 has 4 isomers: P49238-1: 40396 Da (predicted); P49238-2: 43935 Da (predicted); P49238-3: 41062 Da (predicted); P49238-4: 43969 Da (predicted).
    This protein is not N-glycosylated which is unusual for G-protein-coupled receptors

  • Subunit

    Found in a ternary complex with CX3CL1 and ITGAV:ITGB3 or ITGA4:ITGB1

  • SwissProt ID

    P49238

  • Gene ID
    1524 [NCBI]
  • Synonyms

    V28; CCRL1; GPR13; CMKDR1; GPRV28; CMKBRL1

CX3CR1 Antibody (YA4051) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HL-60 cells labeling CX3CR1 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling CX3CR1 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CX3CR1 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L (green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • CX3CR1 Antibody (YA4051) Product Image
    1/16
  • Experimental Validation Results for CX3CR1 Antibody (YA4051)
  • Experimental Validation Results for CX3CR1 Antibody (YA4051)
  • CX3CR1 Antibody (YA4051) Product Image
  • Experimental Validation Results for CX3CR1 Antibody (YA4051)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HL-60 cells labeling CX3CR1 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CX3CR1 Antibody (YA4051)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling CX3CR1 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CX3CR1 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L (green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)
  • Experimental Validation Results for CX3CR1 Antibody (YA4051)
  • Experimental Validation Results for CX3CR1 Antibody (YA4051)
  • CX3CR1 Antibody (YA4051) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 HL-60 cells labeling CX3CR1 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CX3CR1 Antibody (YA4051) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of Hela cells labeling CX3CR1 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with CX3CR1 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L (green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • CX3CR1 Antibody (YA4051) Product Image FC
  • CX3CR1 Antibody (YA4051) Product Image ICC