Cyclin E1 Antibody (YA3902)(PBS only)
(Synonyms: CCNE; Pccne1)Cyclin E1 Antibody (YA3902) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cyclin E1.
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Host:
Mouse
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Isotype:
IgG1
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Application:
IHC-P, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
Cyclin E1 Antibody (YA3902) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cyclin E1.
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Host Mouse
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 47 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 47 kDa
Purified recombinant fragment of human CCNE1 (AA: 1-100) expressed in E. Coli.
affinity purified.
Non-conjugated
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Cyclin E1 is essential for the control of the cell cycle at the G1/S (start) transition
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:It is highly expressed in the testes and placenta. Its expression level is lower in bronchial epithelial cells. -
Isoforms & Post-Translational Modification
P24864 has 3 isomers: P24864-1: 47077 Da (predicted); P24864-2: 41982 Da (predicted); P24864-3: 45150 Da (predicted).
Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7;Ubiquitinated by UHRF2; appears to occur independently of phosphorylation -
Subunit
Interacts with CDK2 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex (PubMed:15660127). Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1 (PubMed:15178429). Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation (PubMed:21952639). Interacts with INCA1 (PubMed:21540187)
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SwissProt ID
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Synonyms
CCNE; Pccne1
Documentation
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