Gli1 Antibody (YA3698)
(Synonyms: GLI)Gli1 Antibody (YA3698) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Gli1.
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Host:
Mouse
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Isotype:
IgG1
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Application:
IHC-P, ICC/IF, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200 - 1:1000 | 1:200-1:400 | 1:10000 |
Product Details
Gli1 Antibody (YA3698) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Gli1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 118 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 118 kDa
Purified recombinant fragment of human GLI1 (AA: 284-449) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Gli1 acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling; Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration[1][2][3][4][5][6][7][8][9][10].
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Subcellular Localization
Cytoplasm; Nucleus; Cytoplasm; Nucleus
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Expression
Tissue_specificity:Protein expression was detected in the testes (PubMed: 2105456) . It was also expressed in the testes, uterine myometrium, and fallopian tubes. Expression was also found in brain tissue, with the highest expression levels in the cerebellum, optic nerve, and olfactory tract (PubMed: 19878745) . Isomer 1 was detected in the brain, spleen, pancreas, liver, kidney, and placenta; isomer 2 was not detected in these tissues (PubMed: 19706761) .
Induction:Isoform 1 and isoform 2 are amplified in glioblastoma cells -
Subunit
Interacts with STK36 (PubMed:10806483). Interacts with ZIC1; the interaction enhances transcription activation (PubMed:11238441)
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SwissProt ID
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Synonyms
GLI
Documentation
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Data Sheet (234 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
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