IRAKM Antibody (YA4596)(PBS only)

Cat. No.: HY-P84899A: Data Sheet User Guide for Antibodies
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IRAKM Antibody (YA4596) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IRAKM.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

IRAKM Antibody (YA4596) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IRAKM.

Host

Mouse

Molecular Weight

Predicted band size: 68 kDa;

Observed band size: 68 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9Y616

Gene ID

11213 [NCBI]

Immunogen

Purified recombinant fragment of human IRAK3 (AA: 454-596) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Putative inactive protein kinase which regulates signaling downstream of immune receptors including IL1R and Toll-like receptors (PubMed:10383454, PubMed:29686383). Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex (By similarity). Upon IL33-induced lung inflammation, positively regulates expression of IL6, CSF3, CXCL2 and CCL5 mRNAs in dendritic cells (PubMed:29686383)

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:It is expressed (protein level) in eosinophils, dendritic cells, and/or monocytes (PubMed: 29686383) . It is primarily expressed in peripheral blood lymphocytes (PubMed: 10383454) .

Isoforms & Post-Translational Modification：Q9Y616 has 2 isomers: Q9Y616-1: 67767 Da (predicted); Q9Y616-2: 60608 Da (predicted).

Subunit：Monomer (PubMed:33238146). Homodimer; disulfide-linked (PubMed:33238146). May interact with IRAK4 (when phosphorylated) (PubMed:33238146). Interacts (when phosphorylated at Ser-110) with PIN1 (via WW domain) in response to IL33-mediated (but not TLR4 ligand LPS) dendritic cell stimulation (PubMed:29686383)

Synonyms

ASRT5; IRAKM

Documentation

User Guide for Antibodies (1077 KB)