PARP16 Antibody (YA8028)(PBS only)

Cat. No.: HY-P88344A: Data Sheet User Guide for Antibodies
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PARP16 Antibody (YA8028) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PARP16.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PARP16 Antibody (YA8028) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PARP16.

Host

Mouse

Molecular Weight

Predicted band size: 36.4 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q8N5Y8

Immunogen

Full-length protein expressed in 293T cell transfected with human PARP16 expression vector .

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Intracellular mono-ADP-ribosyltransferase that plays a role in different processes, such as protein translation and unfolded protein response (UPR), through the mono-ADP-ribosylation of proteins involved in those processes (PubMed:22701565, PubMed:23103912, PubMed:25043379, PubMed:34314702). Acts as an inhibitor of protein translation by catalyzing mono-ADP-ribosylation of ribosomal subunits, such as RPL14 and RPS6, thereby inhibiting polysome assembly and mRNA loading (PubMed:34314702). Mono-ADP-ribosylation of ribosomal subunits is promoted by NMNAT2 (PubMed:34314702). Involved in the unfolded protein response (UPR) by ADP-ribosylating and activating EIF2AK3 and ERN1, two important UPR effectors (PubMed:23103912). May also mediate mono-ADP-ribosylation of karyopherin KPNB1 a nuclear import factor (PubMed:22701565). May not modify proteins on arginine or cysteine residues compared to other mono-ADP-ribosyltransferases (PubMed:22701565)

Subcellular Localization：Endoplasmic reticulum membrane

Isoforms & Post-Translational Modification：Q8N5Y8 has three isomers: Q8N5Y8-1: 36383 Da (predicted); Q8N5Y8-2: 23378 Da (predicted); Q8N5Y8-3: 36470 Da (predicted).
Auto-mono-ADP-ribosylated

Subunit：Interacts with KPNB1

Synonyms

ARTD15; C15orf30; pART15

Documentation

User Guide for Antibodies (1077 KB)