PHLPP2 Antibody (YA1093)
(Synonyms: PHLPP2; KIAA0931, PHLPPL; PH domain leucine-rich repeat-containing protein phosphatase 2; PH domain leucine-rich repeat-containing protein phosphatase-like (PHLPP-like))
Based on 1 Customer Validation
PHLPP2 Antibody (YA1093) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHLPP2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
IHC-P
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Reactivity :
Human
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|
| Dilution Ratio | 1:100-1:200 |
Product Details
PHLPP2 Antibody (YA1093) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHLPP2.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 147 kDa
A synthesized peptide derived from human PHLPP2
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PHLPP2 is a Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity[1][2][3].
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Subcellular Localization
Cytoplasm; Membrane; Peripheral membrane protein; Nucleus
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Expression
Tissue_specificity:In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level) -
Isoforms & Post-Translational Modification
Q6ZVD8 has 3 isomers: Q6ZVD8-1: 146751 Da (predicted); Q6ZVD8-2: 107079 Da (predicted); Q6ZVD8-3: 139394 Da (predicted).
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Subunit
Interacts with AKT1, AKT3 and PRKCB isoform beta-II (PubMed:17386267, PubMed:18162466, PubMed:19732725). Interacts with STK4, RPS6KB1, RAF1 (PubMed:20513427, PubMed:21986499, PubMed:24530606). Interacts with FKBP5; FKBP5 acts as a scaffold for PHLPP2 and Akt (PubMed:19732725). Interacts with NHERF1; NHERF1 scaffolds a heterotrimeric complex with PTEN (PubMed:21804599)
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SwissProt ID
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Synonyms
PHLPP2; KIAA0931, PHLPPL; PH domain leucine-rich repeat-containing protein phosphatase 2; PH domain leucine-rich repeat-containing protein phosphatase-like (PHLPP-like)
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Research Field
Signal Transduction
Documentation
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