PHLPP2 Antibody (YA1093)

製品番号: HY-P81348: データシート COA User Guide for Antibodies
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PHLPP2 Antibody (YA1093) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHLPP2.

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容量	価格（税別）	在庫状況	数量
20 μL	$146	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
説明
参考文献

製品説明

PHLPP2 Antibody (YA1093) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHLPP2.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 147 kDa

Species Reactivity

Human

SwissProt ID

Q6ZVD8

Gene ID

23035 [NCBI]

Immunogen

A synthesized peptide derived from human PHLPP2

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	100-1:200

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Background

Function：Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed:20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed:21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (PubMed:24530606)

Subcellular Localization：Cytoplasm; Membrane; Peripheral membrane protein; Nucleus

Expression：
Tissue_specificity:In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level)

Isoforms & Post-Translational Modification：Q6ZVD8 has 3 isomers: Q6ZVD8-1: 146751 Da (predicted); Q6ZVD8-2: 107079 Da (predicted); Q6ZVD8-3: 139394 Da (predicted).

Subunit：Interacts with AKT1, AKT3 and PRKCB isoform beta-II (PubMed:17386267, PubMed:18162466, PubMed:19732725). Interacts with STK4, RPS6KB1, RAF1 (PubMed:20513427, PubMed:21986499, PubMed:24530606). Interacts with FKBP5; FKBP5 acts as a scaffold for PHLPP2 and Akt (PubMed:19732725). Interacts with NHERF1; NHERF1 scaffolds a heterotrimeric complex with PTEN (PubMed:21804599)

Database

Entrez Gene: 23035 Human

SwissProt: Q6ZVD8 Human

OMIM: 611066 Human

Research Field

Signal Transduction

Synonyms

PHLPP2; KIAA0931, PHLPPL; PH domain leucine-rich repeat-containing protein phosphatase 2; PH domain leucine-rich repeat-containing protein phosphatase-like (PHLPP-like)

ドキュメンテーション

Select Batch:

COA (206 KB)

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/scc-3-cell.html