Phospho-eIF4EBP1 (Ser65) Antibody (YA4245)(PBS only)
(Synonyms: EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I)Phospho-eIF4EBP1 (Ser65) Antibody (YA4245) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Phospho-eIF4EBP1 (Ser65).
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Host:
Mouse
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Isotype:
IgG1
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Application:
IHC-P, ICC/IF, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:100-1:500 | 1:200-1:400 | 1:10000 |
Product Details
Phospho-eIF4EBP1 (Ser65) Antibody (YA4245) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Phospho-eIF4EBP1 (Ser65).
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Host Mouse
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 15-20 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 13 kDa
Synthesized peptide of human Phospho-4E-BP1 (Ser65).(FLMESRNS(p)PVTKTP).
affinity purified.
Non-conjugated
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
eIF4EBP1 is a Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways
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Subcellular Localization
Cytoplasm; Nucleus
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Subunit
Hypophosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation (PubMed:12150926, PubMed:16271312, PubMed:17368478, PubMed:17631896, PubMed:22578813, PubMed:25702871, PubMed:7935836, PubMed:8521827).
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SwissProt ID
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Synonyms
EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
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