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Phospho-MLKL(S358) Antibody (YA5761)

(Synonyms: MLKL)
1 Cited Publications
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Based on 1 publication(s) in Google Scholar

Phospho-MLKL(S358) Antibody (YA5761) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Phospho-MLKL(S358).

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    ICC/IF, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-MLKL(S358) Antibody (YA5761)
  • Phospho-MLKL(S358) Antibody (YA5761) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Publications Citing Use of MedChemExpress (MCE) Phospho-MLKL(S358) Antibody (YA5761)

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1 Publications Citing Use of MCE Phospho-MLKL(S358) Antibody (YA5761) (1)
WB
  • WB

Applications

Application
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:50-200 1:100-200

Product Details

Description

Phospho-MLKL(S358) Antibody (YA5761) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Phospho-MLKL(S358).

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 54 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Immunogen

Synthetic Peptide of phospho-MLKL (S358)

Purification

affinity chromatography.

Conjugation

Non-conjugated

Modification

Phosphorylated

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    MLKL is a Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Does not have protein kinase activity. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following activation by ZBP1, MLKL is phosphorylated by RIPK3 in the nucleus, triggering disruption of the nuclear envelope and leakage of cellular DNA into the cytosol.following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function[1][2][3][4][5].

  • Subcellular Localization

    Cytoplasm; Cell membrane; Nucleus

  • Isoforms & Post-Translational Modification

    Q8NB16 has 2 isomers: Q8NB16-1: 54479 Da (predicted); Q8NB16-2: 30279 Da (predicted).
    Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis (PubMed:22265413). It also induces homotrimerization and localization to the plasma membrane (PubMed:22265413)

  • Subunit

    Homooligomer (PubMed:29883610). Homotrimer; forms homotrimers on necroptosis induction (PubMed:24316671). Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3 (PubMed:22265414). Within this complex, may play a role in the proper targeting of RIPK1-RIPK3 to its downstream effector PGAM5 (PubMed:22265414). Interacts with RIPK3; the interaction is direct and promotes its phosphorylation and subsequent activation (PubMed:22265413, PubMed:22421439, PubMed:29883609)

  • SwissProt ID

    Q8NB16

  • Gene ID
    197259 [NCBI]
  • Synonyms

    MLKL

Phospho-MLKL(S358) Antibody (YA5761) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    Phospho-MLKL(S358) Antibody (YA5761) purchased from MedChemExpress. Usage Cited in: Cells. 2026 Feb 28;15(5):430. [Abstract]

    Customer Validation
     Western blot analysis of key PANoptosis markers, including p-MLKL (necroptosis), N-GSDMD (pyroptosis), and Cleaved-Caspase 3 (apoptosis), in Control, CHP2-OV, and CHP2-OV-siRNA-3 groups. Primary antibodies: CHP2 (1:1000), p-MLKL (Phospho-MLKL(S358) Antibody (YA5761), 1:1000, HY-P86069), GSDMD (N-terminal) (GSDMD (N-terminal) Antibody (YA5502), 1:1000, HY-P85810), Cleaved Caspase-3 (Cleaved Caspase-3 (Asp175) Antibody (YA6062), HY-P86370) and β-actin (1:2000).

  • Phospho-MLKL(S358) Antibody (YA5761) Product Image
    1/16
  • Experimental Validation Results for Phospho-MLKL(S358) Antibody (YA5761)
  • Phospho-MLKL(S358) Antibody (YA5761) Product Image

  • Phospho-MLKL(S358) Antibody (YA5761) purchased from MedChemExpress. Usage Cited in: Cells. 2026 Feb 28;15(5):430. [Abstract]

    Western blot analysis of key PANoptosis markers, including p-MLKL (necroptosis), N-GSDMD (pyroptosis), and Cleaved-Caspase 3 (apoptosis), in Control, CHP2-OV, and CHP2-OV-siRNA-3 groups. Primary antibodies: CHP2 (1:1000), p-MLKL (Phospho-MLKL(S358) Antibody (YA5761), 1:1000, HY-P86069), GSDMD (N-terminal) (GSDMD (N-terminal) Antibody (YA5502), 1:1000, HY-P85810), Cleaved Caspase-3 (Cleaved Caspase-3 (Asp175) Antibody (YA6062), HY-P86370) and β-actin (1:2000).