MLKL - mixed lineage kinase domain like pseudokinase Gene

Also Known as hMLKL

Species: Homo sapiens

Gene Type: protein coding
Gene ID: 197259

About MLKL

Cytogenetic location: 16q23.1 Genomic coordinates (GRCh38): 16:74,671,855-74,700,862 (from NCBI)

This gene has 8 transcripts (splice variants), 178 orthologues and 23 paralogues. Broad expression in spleen (RPKM 10.5), bone marrow (RPKM 10.4) and 23 other tissues.

Summary

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced Necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in Necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]

MLKL Products (2)

mRNA Protein Name
NM_001142497.3 NP_001135969.1 mixed lineage kinase domain-like protein isoform 2
NM_152649.4 NP_689862.1 mixed lineage kinase domain-like protein isoform 1

Gene Ontology

  • Molecular Function
  • Biological Process
  • Cellular Component
Molecular Function GO Annotation Evidence References Source
enables ATP binding IDA
IDA: Inferred from direct assay
24219132 GOA
enables identical protein binding IPI
IPI: Inferred from physical interaction
24316671 GOA
enables protein binding IPI
IPI: Inferred from physical interaction
22265413 GOA
enables protein-containing complex binding IDA
IDA: Inferred from direct assay
22265414 GOA
Biological Process GO Annotation Evidence References Source
involved in execution phase of necroptosis IDA
IDA: Inferred from direct assay
24316671 GOA
involved in necroptotic process IMP
IMP: Inferred from mutant phenotype
22265414 GOA
involved in necroptotic signaling pathway IMP
IMP: Inferred from mutant phenotype
22265413 GOA
involved in protein homotrimerization IDA
IDA: Inferred from direct assay
24316671 GOA
Cellular Component GO Annotation Evidence References Source
located in cytoplasm IDA
IDA: Inferred from direct assay
24316671 GOA
located in plasma membrane IDA
IDA: Inferred from direct assay
24316671 GOA
EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

MLKL Protein Structure

Pkinase_Tyr

Pkinase_Tyr: Protein tyrosine kinase (219 - 466)

  • 0
  • 100
  • 200
  • 300
  • 400
  • 471 a.a.
Protein Preferred Names Protein Names

mixed lineage kinase domain-like protein

MLKL Protein-protein interaction Information

Type
Protein Name Protein ID Interactor Interactor Species Interactor ID Detection Method References
Intra
MLKL Q8NB16 RIPK3 Homo sapiens Q9Y572
Anti Tag CoIP
22265413
Cross: Cross-species interaction Intra: Intraspecies interaction

MLKL Antibodies

Cat. No. Product Name Application Reactivity
HY-P80227 MLKL Antibody (YA289) WB, IHC-P Human, Mouse
HY-P81878 Phospho-MLKL (Ser358) Antibody (YA1623) WB, IHC-P Human
HY-P86069 Phospho-MLKL(S358) Antibody (YA5761) ICC/IF, IHC-P Human
HY-P86228 MLKL Antibody (YA5920) WB, IHC-P, ICC/IF, IP, ELISA Human, Mouse, Rat

Related Diseases

Diseases Alias
Maturity-Onset Diabetes Of The Young

  • MODY

  • Maturity Onset Diabetes Mellitus In Young

  • Mason-Type Diabetes

  • Mason Type Diabetes

  • Maturity Onset Diabetes Of The Young

  • Mody Syndrome

  • Diabetes Of The Young, Maturity-Onset
Inflammatory Bowel Disease

  • Inflammatory Bowel Diseases

  • Bowel Disease, Inflammatory
Caspase 8 Deficiency

  • Autoimmune Lymphoproliferative Syndrome Type 2b

  • Caspase-8 Deficiency

  • Ceds

  • Alps2b

  • Autoimmune Lymphoproliferative Syndrome, Type Iib

  • Alps With Recurrent Viral Infections

  • Autoimmune Lymphoproliferative Syndrome With Recurrent Viral Infections

  • Caspase 8 Deficiency Syndrome

  • Caspase 8 Lymphadenopathy Syndrome

  • Autoimmune Lymphoproliferative Syndrome Type Iib

  • Caspase Eight Deficiency State

  • CASP8D
Autoimmune Lymphoproliferative Syndrome

  • ALPS

  • Canale-Smith Syndrome

  • Autoimmune Lymphoproliferative Syndrome, Type Ia

  • Autoimmune Lymphoproliferative Syndrome, Type Ib

  • Autoimmune Lymphoproliferative Syndrome Type 1, Autosomal Dominant

  • Css

  • Autoimmune Lymphoproliferative Syndrome, Type 1b

  • Autoimmune Lymphoproliferative Syndrome, Type 1a

  • Autoimmune Lymphoproliferative Syndrome, Type I, Autosomal Dominant

  • Fas Deficiency

  • Autoimmune Lymphoproliferative Syndrome 1a

  • ALPS1A

  • Autoimmune Lymphoproliferative Syndrome Type Ia

  • Autoimmune Lymphoproliferative Syndrome 1b

  • ALPS1B

  • Autoimmune Lymphoproliferative Syndrome Type Ib
Amyotrophic Lateral Sclerosis 1

  • Amyotrophic Lateral Sclerosis

  • ALS

  • Lou Gehrig Disease

  • Amyotrophic Lateral Sclerosis Type 1

  • Charcot Disease

  • ALS1

  • Amyotrophic Lateral Sclerosis, Susceptibility To

  • Fals

  • Lou Gehrig'S Disease

  • Mnd

  • Motor Neuron Disease

  • Familial Amyotrophic Lateral Sclerosis

  • Amyotrophic Lateral Sclerosis 1, Familial

  • Amyotrophic Lateral Sclerosis 1, Autosomal Dominant

  • Motor Neuron Disease, Bulbar

  • Motor Neurone Disease

  • Amyotrophic Lateral Sclerosis With Dementia

  • Dementia With Amyotrophic Lateral Sclerosis

  • Motor Neuron Disease, Amyotrophic Lateral Sclerosis

  • Sclerosis, Lateral, Amyotrophic

  • Sclerosis, Lateral, Amyotrophic, Type 1

  • Amyotrophic Sclerosis

  • Als - [Amyotrophic Lateral Sclerosis]

  • Wasting Palsy

  • Amyotrophic Paralysis

  • Amyotrophy Lateral Sclerosis

  • Wasting Paralysis

  • Spinal Progressive Amyotrophy

  • Progressive Atrophic Paralysis
Diseases Alias
Waldenstroem'S Macroglobulinemia

Waldenstroem'S Macroglobulinemia

Waldenstroem'S Macroglobulinemia

Macroglobulinemia Of Waldenstrom

Lymphoplasmacytic Lymphoma With Igm Gammopathy

Lymphoplasmacytic Lymphoma

Waldenstroem'S Macroglobulinemia

Waldenstroem'S Macroglobulinemia

Macroglobulinemia Of Waldenstrom

Lymphoplasmacytic Lymphoma With Igm Gammopathy

Lymphoplasmacytic Lymphoma

Related Agents

  • Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (57)
Cat. No. Product Name
Effect
Purity
Rare Diseases
HY-100573 Necrosulfonamide 98.61% Unkown
HY-10587 BIX-01294 98.44% Unkown
HY-15594A Phen-DC3 Trifluoromethanesulfonate 98.64% Unkown
HY-112292 GW806742X Inhibitor 99.91% Unkown
HY-144828 RIP1/RIP3/MLKL activator 1 97.0% Unkown
HY-101524 TC13172 Inhibitor 99.04% Unkown
HY-162424 ZBP1/RIP3/MLKL activator 1 Activator 99.73% Unkown
HY-N0660 Jujuboside B 99.92% Unkown
HY-156591 PROTAC MLKL Degrader-1 99.76% Unkown
HY-N2909 Aurantiamide Inhibitor 99.56% Unkown
HY-112292A GW806742X hydrochloride Inhibitor 98.30% Unkown
HY-N0546 Ligustroflavone 99.90% Unkown
HY-108239 BIX-01294 trihydrochloride 99.68% Unkown
HY-156119 MLKL-IN-6 Inhibitor 98.57% Unkown
HY-141889 MLKL-IN-2 Inhibitor 99.45% Unkown
HY-157039 RIPK1-IN-17 Inhibitor 97.09% Unkown
HY-149393 RIPK3-IN-3 Inhibitor 95.98% Unkown
HY-100573A (E/Z)-Necrosulfonamide 98.03% Unkown
HY-108636 RETRA 99.42% Unkown
HY-100573S Necrosulfonamide-d4 99.65% Unkown
HY-148365 NecroIr1 98.53% Unkown
HY-148366 NecroIr2 98.02% Unkown
HY-RS08492 MLKL Human Pre-designed siRNA Set A Pre-designed Set / Unkown
HY-177119 ZBP1 Covalent PROTAC-1 96.87% Unkown
HY-139878 MLKL-IN-1 Inhibitor / Unkown
HY-148767 MLKL-IN-5 Inhibitor / Unkown
HY-149052 SZM-1209 Inhibitor / Unkown
HY-151541 MLKL-IN-3 Inhibitor / Unkown
HY-161361 MLKL-IN-7 Inhibitor / Unkown
HY-168640 RIP3 activator 1 / Unkown
HY-169072 PROTAC MLKL Degrader-2 / Unkown
HY-169073 TC13172-NH2 Ligand / Unkown
HY-169074 Thalidomide-N-methylpiperazine / Unkown
HY-169509 Topoisomerase I/II inhibitor 8 / Unkown
HY-173075 Anticancer agent 267 / Unkown
HY-173185 RIP1-IN-1 Inhibitor / Unkown
HY-175705 MLKL ligand-1 Ligand / Unkown
HY-176407 ZYH-23 Inhibitor / Unkown
HY-178163 Zharp1-163 Inhibitor / Unkown
HY-178464 RIPK1-IN-34 Inhibitor / Unkown
HY-179680 RIPK1-IN-38 Inhibitor / Unkown
HY-180806 RIPK1-IN-39 Inhibitor / Unkown
HY-181596 WJH-C19 Inhibitor 98.65% Unkown
HY-182066 Photosensitizer-9 Activator / Unkown
HY-182343 PROTAC MLKL Degrader-3 / Unkown
HY-182344 MLKL ligand-2 Ligand / Unkown
HY-183538 Necroptosis inducer 2 / Unkown
HY-183564 RIPK1/RIPK3-PPI-IN-1 Inhibitor / Unkown
HY-183781 RIPK1-IN-41 Inhibitor / Unkown
HY-184686 RIPK1-IN-42 Inhibitor / Unkown
HY-N0660R Jujuboside B (Standard) / Unkown
HY-N20708 Chiisanogenin / Unkown
HY-151542 MLKL-IN-4 Inhibitor / Unkown
HY-N8380 (-)-Latifolin / Unkown
HY-RS16563 Mlkl Mouse Pre-designed siRNA Set A Pre-designed Set / Unkown
HY-RS22995 Mlkl Rat Pre-designed siRNA Set A Pre-designed Set / Unkown
HY-15594 Phen-DC3 / Unkown

Orthologs Information

Species Symbol Source ID
Rattus norvegicus MLKL RGD RGD:1592221
Mus musculus MLKL MGD MGI:1921818
Bos taurus MLKL VGNC VGNC:31500
Macaca mulatta MLKL VGNC VGNC:82188
Others MLKL NCBI