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Cat. No.: HY-151542

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MLKL-IN-4

MLKL-IN-4 (compound 56) is a potent MLKL (Mixed lineage kinase domain-like protein) inhibitor. MLKL-IN-4 inhibits necroptosis in HT-29 cells and acts downstream of MLKL phosphorylation, with EC₅₀ of 82 nM. MLKL-IN-4 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

For research use only. We do not sell to patients.

CAS No.: 3031406-17-1
Formula: C₃₀H₂₇ClN₄O₅
Molecular Weight:559.01

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (265 KB)

MLKL-IN-4 Chemical Structure

CAS No.: 3031406-17-1

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

MLKL-IN-4 (compound 56) is a potent MLKL (Mixed lineage kinase domain-like protein) inhibitor. MLKL-IN-4 inhibits necroptosis in HT-29 cells and acts downstream of MLKL phosphorylation, with EC₅₀ of 82 nM^[1]. MLKL-IN-4 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

All RIP kinase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HT-29	EC₅₀	>10 μM Compound: 56	Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 5 min followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 5 min followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay	[PMID: 36136378]
HT-29	EC₅₀	180.3 nM Compound: 56	Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 3 hr followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 3 hr followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay	[PMID: 36136378]
HT-29	EC₅₀	188.1 nM Compound: 56	Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 3 hrs followed by TSZ stimulation by celltiter-glo luminescent cell viability assay Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 3 hrs followed by TSZ stimulation by celltiter-glo luminescent cell viability assay	[PMID: 36136378]
HT-29	EC₅₀	584.3 nM Compound: 56	Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 1 hr followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ induced necroptosis incubated for 1 hr followed by compound washout and further induced with TSZ by celltiter-glo luminescent cell viability assay	[PMID: 36136378]
HT-29	EC₅₀	82 nM Compound: 56	Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ (TNFalpha, Smac mimetic and z-VAD-FMK) induced necroptosis incubated for 24 hrs by celltiter-glo luminescent cell viability assay Anti-necroptic activity in human HT-29 cells assessed as inhibition of TSZ (TNFalpha, Smac mimetic and z-VAD-FMK) induced necroptosis incubated for 24 hrs by celltiter-glo luminescent cell viability assay	[PMID: 36136378]

In Vitro

MLKL-IN-4 (compound 56) (10 μM) slightly inhibits RIPK1, does not affect the phosphorylation status of RIPK1 and MLKL^[1].
MLKL-IN-4 (1 μM, 24 h) inhibits the translocation of MLKL to cell membranes in HT-29 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

MLKL-IN-4 (compound 56) shows T1/2 over 48 h and more than 150-fold lower reaction rates with glutathione (GSH), which potentially decreased their off-target effects and cell toxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 3031406-17-1
Molecular Weight 559.01
Formula C₃₀H₂₇ClN₄O₅
SMILES

O=C(NC1=C(Cl)N(CC#CC2=CC=C(O)C(CN3C=CC=CC3=O)=C2)C(N(C)C1=O)=O)CCC4=CC=C(C)C=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (265 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Cui B, et al. Discovery of a New Class of Uracil Derivatives as Potential Mixed Lineage Kinase Domain-like Protein (MLKL) Inhibitors. J Med Chem. 2022 Oct 13;65(19):12747-12780. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References

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MLKL-IN-4

All RIP kinase Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

MLKL-IN-4 Related Classifications

HY-151542 Related Classifications