Phen-DC3

Cat. No.: HY-15594: Data Sheet Handling Instructions
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Phen-DC3 is a G-quadruplex (G4) specific ligand which can inhibit FANCJ and DinG helicases with IC₅₀s of 65±6 and 50±10 nM, respectively.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (Phen-DC3 Trifluoromethanesulfonate) that retains the same biological activity.

For research use only. We do not sell to patients.

CAS No. : 942936-75-6

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This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of Phen-DC3:

Phen-DC3 Trifluoromethanesulfonate

Other Forms of Phen-DC3:

Phen-DC3 Trifluoromethanesulfonate In-stock

9 Publications Citing Use of MCE Phen-DC3

RT-PCR

Bio/Physico-chemical Assay

Cell Proliferation/Viability Assay

Flow Cytometry

: Phen-DC3 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jun 20;319(Pt 1):145405. [Abstract]; FRET-MC profiles were obtained under different experimental conditions: (1) 0.2 μM F21T alone (black line), (2) 0.2 μM F21T with 0.4 μM Phen-DC3 Trifluoromethanesulfonate (PhenDC3) (red line), (3) 0.2 μM F21T with 0.4 μM PhenDC3 and 2 μM SOST RG4wt competitors (blue line), and (4) 0.2 μM F21T with 0.4 μM PhenDC3 and 2 μM SOST RG4mut competitors (purple line). The FRET-MC buffer was composed of 10 mM KCl, 90 mM LiCl, and 10 mM lithium cacodylate at pH 7.2.

: Phen-DC3 purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105312.; Cell viability was analyzed using a CCK-8 kit under the G4 stabilizer treatment for 12 h, 24 h, and 48 h. Phen-DC3 Trifluoromethanesulfonate (Phen-DC3) (5-100 nM)-induced G4 stabilization inhibited NP cell viability in a dose-dependent manner.

: Phen-DC3 purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105312.; mRNA expression of Il6 after the single or combined treatment of PDS, Braco-19, Phen-DC3 Trifluoromethanesulfonate (Phen-DC3) (5-20 nM) and IL-1β (10 ng/mL). Phen-DC3 promoted Il6 mRNA expression in NP cells in a dose-dependent manner by stabilizing genomic G4 structures.

: Phen-DC3 purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105312.; Flow cytometry assay for the cell apoptosis under the treatment of PDS, Braco-19, and Phen-DC3 Trifluoromethanesulfonate (Phen-DC3) (5-20 nM).

: Phen-DC3 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2021 Oct 25;12(11):999. [Abstract]; The relative enrichment of G-quadruplexes (G4) in Lhcgr CGI-II from TM3 cells treated with vehicle or 10 μM 5-ALA, 5 μM 360 A iodide, or 10 μM Phen-DC3 Trifluoromethanesulfonate (Phen-DC3) (48 h) by ChIP-qPCR analysis. The G-quadruplex antibody (BG4) or IgG were used, respectively. N = 3 for each group.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Phen-DC3 is a G-quadruplex (G4) specific ligand which can inhibit FANCJ and DinG helicases with IC₅₀s of 65±6 and 50±10 nM, respectively.

IC₅₀ & Target

Helicase

Cellular Effect

Cell Line	Type	Value	Description	References
HEK-293T	IC₅₀	1.5 nM Compound: phen-DC3	Inhibition of human telomerase expressed in HEK293T cells treated before telomerase elongation by telomeric repeat amplification protocol assay Inhibition of human telomerase expressed in HEK293T cells treated before telomerase elongation by telomeric repeat amplification protocol assay	[PMID: 17954919]
HEK-293T	IC₅₀	1500 nM Compound: phen-DC3	Inhibition of human telomerase expressed in HEK293T cells in presence of primer TS by primer extension assay Inhibition of human telomerase expressed in HEK293T cells in presence of primer TS by primer extension assay	[PMID: 17954919]
HEK-293T	IC₅₀	2.2 nM Compound: phen-DC3	Inhibition of human telomerase expressed in HEK293T cells treated after telomerase elongation by telomeric repeat amplification protocol assay Inhibition of human telomerase expressed in HEK293T cells treated after telomerase elongation by telomeric repeat amplification protocol assay	[PMID: 17954919]
HEK-293T	IC₅₀	63 nM Compound: phen-DC3	Inhibition of human telomerase expressed in in HEK293T cells in presence of primer (T2AG3)4 by primer extension assay Inhibition of human telomerase expressed in in HEK293T cells in presence of primer (T2AG3)4 by primer extension assay	[PMID: 17954919]
HEK-293T	IC₅₀	790 nM Compound: phen-DC3	Inhibition of human telomerase expressed in HEK293T cells in presence of primer (T2AG3)3 by primer extension assay Inhibition of human telomerase expressed in HEK293T cells in presence of primer (T2AG3)3 by primer extension assay	[PMID: 17954919]

Molecular Weight

550.61

Formula

C₃₄H₂₆N₆O₂

CAS No.

942936-75-6

SMILES

C[N+]1=C2C=CC=CC2=CC(NC(C3=CC=C4C=CC5=CC=C(C(NC6=C[N+](C)=C7C=CC=CC7=C6)=O)N=C5C4=N3)=O)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : ≥ 34 mg/mL (61.75 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8162 mL	9.0808 mL	18.1617 mL
5 mM	0.3632 mL	1.8162 mL	3.6323 mL
10 mM	0.1816 mL	0.9081 mL	1.8162 mL

View the Complete Stock Solution Preparation Table

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Bharti SK, et al. Specialization among iron-sulfur cluster helicases to resolve G-quadruplex DNA structures that threaten genomic stability. J Biol Chem. 2013 Sep 27; 288(39):28217-29.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8162 mL	9.0808 mL	18.1617 mL	45.4042 mL
	5 mM	0.3632 mL	1.8162 mL	3.6323 mL	9.0808 mL
	10 mM	0.1816 mL	0.9081 mL	1.8162 mL	4.5404 mL
	15 mM	0.1211 mL	0.6054 mL	1.2108 mL	3.0269 mL
	20 mM	0.0908 mL	0.4540 mL	0.9081 mL	2.2702 mL
	25 mM	0.0726 mL	0.3632 mL	0.7265 mL	1.8162 mL
	30 mM	0.0605 mL	0.3027 mL	0.6054 mL	1.5135 mL
	40 mM	0.0454 mL	0.2270 mL	0.4540 mL	1.1351 mL
	50 mM	0.0363 mL	0.1816 mL	0.3632 mL	0.9081 mL
	60 mM	0.0303 mL	0.1513 mL	0.3027 mL	0.7567 mL

Phen-DC3 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phen-DC3942936-75-6DNA/RNA SynthesisInhibitorinhibitorinhibit

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