Cat. No.: HY-15594

Phen-DC3

12 Cited Publications

Customer Review

Based on 12 publication(s) in Google Scholar

Phen-DC3 is a G-quadruplex (G4) specific ligand which can inhibit FANCJ and DinG helicases with IC₅₀s of 65±6 and 50±10 nM, respectively.

For research use only. We do not sell to patients.

CAS No.: 942936-75-6
Formula: C₃₄H₂₆N₆O₂
Molecular Weight:550.61

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (274 KB)

Phen-DC3 Chemical Structure

CAS No.: 942936-75-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
Chemical Information
Publications (12)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Phen-DC3 is a G-quadruplex (G4) specific ligand which can inhibit FANCJ and DinG helicases with IC₅₀s of 65±6 and 50±10 nM, respectively.

IC₅₀ & Target

Helicase

All DNA/RNA Synthesis Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK-293T	IC₅₀	1.5 nM Compound: phen-DC3	Inhibition of human telomerase expressed in HEK293T cells treated before telomerase elongation by telomeric repeat amplification protocol assay Inhibition of human telomerase expressed in HEK293T cells treated before telomerase elongation by telomeric repeat amplification protocol assay	[PMID: 17954919]
HEK-293T	IC₅₀	1500 nM Compound: phen-DC3	Inhibition of human telomerase expressed in HEK293T cells in presence of primer TS by primer extension assay Inhibition of human telomerase expressed in HEK293T cells in presence of primer TS by primer extension assay	[PMID: 17954919]
HEK-293T	IC₅₀	2.2 nM Compound: phen-DC3	Inhibition of human telomerase expressed in HEK293T cells treated after telomerase elongation by telomeric repeat amplification protocol assay Inhibition of human telomerase expressed in HEK293T cells treated after telomerase elongation by telomeric repeat amplification protocol assay	[PMID: 17954919]
HEK-293T	IC₅₀	63 nM Compound: phen-DC3	Inhibition of human telomerase expressed in in HEK293T cells in presence of primer (T2AG3)4 by primer extension assay Inhibition of human telomerase expressed in in HEK293T cells in presence of primer (T2AG3)4 by primer extension assay	[PMID: 17954919]
HEK-293T	IC₅₀	790 nM Compound: phen-DC3	Inhibition of human telomerase expressed in HEK293T cells in presence of primer (T2AG3)3 by primer extension assay Inhibition of human telomerase expressed in HEK293T cells in presence of primer (T2AG3)3 by primer extension assay	[PMID: 17954919]

Chemical Information

CAS No. 942936-75-6
Molecular Weight 550.61
Formula C₃₄H₂₆N₆O₂
SMILES

C[N+]1=C2C=CC=CC2=CC(NC(C3=CC=C4C=CC5=CC=C(C(NC6=C[N+](C)=C7C=CC=CC7=C6)=O)N=C5C4=N3)=O)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (12)

All RT-PCRBio/Physico-chemical AssayCell Proliferation/Viability AssayFlow Cytometry

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Journal Impact Factor

Nucleic Acids Res

RNA G-quadruplexes promote codon repeat-associated ribosomal frameshifting in human genes. [Abstract]

2026 Jan 14;54(2):gkaf1481. PMID: 41543171
Nucleic Acids Res

G-quadruplexes on chromosomal DNA negatively regulates topoisomerase 1 activity. [Abstract]

2024 Mar 21;52(5):2142-2156. PMID: 38340342
Cell Death Dis

MeCP2 duplication causes hyperandrogenism by upregulating LHCGR and downregulating RORα. [Abstract]

2021 Oct 25;12(11):999. PMID: 34697294

Phen-DC3 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2021 Oct 25;12(11):999. [Abstract]

The relative enrichment of G-quadruplexes (G4) in Lhcgr CGI-II from TM3 cells treated with vehicle or 10 μM 5-ALA, 5 μM 360 A iodide, or 10 μM Phen-DC3 Trifluoromethanesulfonate (Phen-DC3) (48 h) by ChIP-qPCR analysis. The G-quadruplex antibody (BG4) or IgG were used, respectively. N = 3 for each group.
Int J Biol Macromol

An RNA G-quadruplex within the sclerostin 3' untranslated region enhances sclerostin expression by blocking miR-4648 binding. [Abstract]

2025 Jun 20;319(Pt 1):145405. PMID: 40545075

Phen-DC3 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jun 20;319(Pt 1):145405. [Abstract]

FRET-MC profiles were obtained under different experimental conditions: (1) 0.2 μM F21T alone (black line), (2) 0.2 μM F21T with 0.4 μM Phen-DC3 Trifluoromethanesulfonate (PhenDC3) (red line), (3) 0.2 μM F21T with 0.4 μM PhenDC3 and 2 μM SOST RG4wt competitors (blue line), and (4) 0.2 μM F21T with 0.4 μM PhenDC3 and 2 μM SOST RG4mut competitors (purple line). The FRET-MC buffer was composed of 10 mM KCl, 90 mM LiCl, and 10 mM lithium cacodylate at pH 7.2.
Int J Biol Macromol

I-motif formation in the promoter region of the B-MYB proto-oncogene. [Abstract]

2025 Mar:296:139582. PMID: 39798757
J Mol Med (Berl)

The role and mechanism of TNFRSF21 in promoting necroptosis of vascular endothelial cells and inducing vascular leakage in sepsis. [Abstract]

2026 Apr 23;104(1):68. PMID: 42020785
iScience

2026 May 22;29(6):116036.
iScience

Genomic G-quadruplex folding triggers a cytokine-mediated inflammatory feedback loop to aggravate inflammatory diseases. [Abstract]

2022 Oct 9;25(11):105312. PMID: 36304116

Phen-DC3 purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105312. [Abstract]

Cell viability was analyzed using a CCK-8 kit under the G4 stabilizer treatment for 12 h, 24 h, and 48 h. Phen-DC3 Trifluoromethanesulfonate (Phen-DC3) (5-100 nM)-induced G4 stabilization inhibited NP cell viability in a dose-dependent manner.

Phen-DC3 purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105312. [Abstract]

mRNA expression of Il6 after the single or combined treatment of PDS, Braco-19, Phen-DC3 Trifluoromethanesulfonate (Phen-DC3) (5-20 nM) and IL-1β (10 ng/mL). Phen-DC3 promoted Il6 mRNA expression in NP cells in a dose-dependent manner by stabilizing genomic G4 structures.

Phen-DC3 purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105312. [Abstract]

Flow cytometry assay for the cell apoptosis under the treatment of PDS, Braco-19, and Phen-DC3 Trifluoromethanesulfonate (Phen-DC3) (5-20 nM).
J Phys Chem B

Nonselective Intercalation of G-Quadruplex-Targeting Ligands into Double-Stranded DNA Quantified by Single-Molecule Stretching. [Abstract]

2023 Jul 6;127(26):5859-5868. PMID: 37357414
Biochem Biophys Res Commun

Targeting DHODH promoter G-quadruplex to induce ferroptosis in melanoma cells. [Abstract]

2026 Jan 8:795:153117. PMID: 41380445
Res Vet Sci

HnRNP H1 binds to nucleocapsid protein and potential guanine-quadruplex site of viral RNA to promote PRRSV-2 proliferation. [Abstract]

2026 Jul:206:106188. PMID: 41996971
Patent

US20210188889A1.

Solvent & Solubility

In Vitro:

DMSO : ≥ 34 mg/mL (61.75 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8162 mL

9.0808 mL

18.1617 mL

5 mM

0.3632 mL

1.8162 mL

3.6323 mL

10 mM

0.1816 mL

0.9081 mL

1.8162 mL

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bharti SK, et al. Specialization among iron-sulfur cluster helicases to resolve G-quadruplex DNA structures that threaten genomic stability. J Biol Chem. 2013 Sep 27; 288(39):28217-29.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.8162 mL	9.0808 mL	18.1617 mL	45.4042 mL
	5 mM	0.3632 mL	1.8162 mL	3.6323 mL	9.0808 mL
	10 mM	0.1816 mL	0.9081 mL	1.8162 mL	4.5404 mL
	15 mM	0.1211 mL	0.6054 mL	1.2108 mL	3.0269 mL
	20 mM	0.0908 mL	0.4540 mL	0.9081 mL	2.2702 mL
	25 mM	0.0726 mL	0.3632 mL	0.7265 mL	1.8162 mL
	30 mM	0.0605 mL	0.3027 mL	0.6054 mL	1.5135 mL
	40 mM	0.0454 mL	0.2270 mL	0.4540 mL	1.1351 mL
	50 mM	0.0363 mL	0.1816 mL	0.3632 mL	0.9081 mL
	60 mM	0.0303 mL	0.1513 mL	0.3027 mL	0.7567 mL

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Phen-DC3

Publications Citing Use of MedChemExpress (MCE) Phen-DC3

All DNA/RNA Synthesis Isoforms

Biological Activity

Chemical Information

Publications (12)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Phen-DC3 Related Classifications

HY-15594 Related Classifications