1. Cell Cycle/DNA Damage
  2. G-quadruplex
  3. Phen-DC3 Trifluoromethanesulfonate

Phen-DC3 Trifluoromethanesulfonate (Synonyms: Phen-DC3 Triflate)

Cat. No.: HY-15594A Purity: 98.66%
Handling Instructions

Phen-DC3 Trifluoromethanesulfonate is a G-quadruplex (G4) specific ligand which can inhibit FANCJ and DinG helicases with IC50s of 65±6 and 50±10 nM, respectively.

For research use only. We do not sell to patients.

Phen-DC3 Trifluoromethanesulfonate Chemical Structure

Phen-DC3 Trifluoromethanesulfonate Chemical Structure

CAS No. : 929895-45-4

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 840 In-stock
Estimated Time of Arrival: December 31
1 mg USD 180 In-stock
Estimated Time of Arrival: December 31
5 mg USD 540 In-stock
Estimated Time of Arrival: December 31
10 mg USD 900 In-stock
Estimated Time of Arrival: December 31
50 mg USD 2700 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Phen-DC3 Trifluoromethanesulfonate is a G-quadruplex (G4) specific ligand which can inhibit FANCJ and DinG helicases with IC50s of 65±6 and 50±10 nM, respectively.

IC50 & Target

IC50: 65±6 nM (G4 substrate, FANCJ helicase), 50±10 nM (G4 substrate, DinG helicases)[1]

In Vitro

In WT cells, a CEB1-WT array is rather stable but undergoes frequent rearrangements upon addition of 10 μM Phen-DC3 Trifluoromethanesulfonate (Phen-DC3). It is found that the c-Myc allele exhibits significant destabilization upon Phen-DC3 Trifluoromethanesulfonate treatment and PIF1 deletion. The CEB25-L111(T) array is stable in WT cells, it becomes unstable upon addition of Phen-DC3 Trifluoromethanesulfonate or deletion of PIF1. It is also highly destabilized in the presence of Phen-DC3 Trifluoromethanesulfonate or in the absence of PIF1. The CEB1-loop CEB25 allele remaines fully stable in both PIF1-treated and WT cells[2].

Molecular Weight

848.75

Formula

C₃₆H₂₆F₆N₆O₈S₂

CAS No.

929895-45-4

SMILES

[O-]S(=O)(C(F)(F)F)=O.C[N+]1=C2C=CC=CC2=CC(NC(C3=CC=C4C=CC5=CC=C(C(NC6=C[N+](C)=C7C=CC=CC7=C6)=O)N=C5C4=N3)=O)=C1.[O-]S(=O)(C(F)(F)F)=O

Shipping

Room temperature in continental US; may vary elsewhere

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 34 mg/mL (40.06 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1782 mL 5.8910 mL 11.7820 mL
5 mM 0.2356 mL 1.1782 mL 2.3564 mL
10 mM 0.1178 mL 0.5891 mL 1.1782 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[2]

Briefly, untreated WT cells and pif1Δ cells from a fresh patch of cells get from a single colony bearing the parental allele size are diluted in 5 mL of YPD (2×105 cells/mL), grown for 8 generations at 30°C with shaking, and spreaded as single colony on YPD plates. To measure minisatellite instability upon Phen-DC3 Trifluoromethanesulfonate treatment, WT cells from a fresh patch on YPD are grown for 8 generations at 30°C in liquid SC containing Phen-DC3 Trifluoromethanesulfonate at 10 μM. Isolated colonies or pools of colonies are analyzed by Southern blot using the EcoRI digestion that cuts at each side of the minisatellite[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Product Name:
Phen-DC3 Trifluoromethanesulfonate
Cat. No.:
HY-15594A
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