2. Cell Cycle/DNA Damage
  3. G-quadruplex
  4. Phen-DC3 Trifluoromethanesulfonate

Phen-DC3 Trifluoromethanesulfonate  (Synonyms: Phen-DC3 Triflate)

Cat. No.: HY-15594A Purity: 98.99%
COA Handling Instructions

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Phen-DC3 Trifluoromethanesulfonate is a G-quadruplex (G4) specific ligand which can inhibit FANCJ and DinG helicases with IC50s of 65±6 and 50±10 nM, respectively.

For research use only. We do not sell to patients.

Phen-DC3 Trifluoromethanesulfonate Chemical Structure

Phen-DC3 Trifluoromethanesulfonate Chemical Structure

CAS No. : 929895-45-4

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1 mg USD 50 In-stock
5 mg USD 105 In-stock
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25 mg USD 285 In-stock
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Based on 5 publication(s) in Google Scholar

Other Forms of Phen-DC3 Trifluoromethanesulfonate:

Phen-DC3 Trifluoromethanesulfonate Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Phen-DC3 Trifluoromethanesulfonate is a G-quadruplex (G4) specific ligand which can inhibit FANCJ and DinG helicases with IC50s of 65±6 and 50±10 nM, respectively.

IC50 & Target

IC50: 65±6 nM (G4 substrate, FANCJ helicase), 50±10 nM (G4 substrate, DinG helicases)[1]
In Vitro

In WT cells, a CEB1-WT array is rather stable but undergoes frequent rearrangements upon addition of 10 μM Phen-DC3 Trifluoromethanesulfonate (Phen-DC3). It is found that the c-Myc allele exhibits significant destabilization upon Phen-DC3 Trifluoromethanesulfonate treatment and PIF1 deletion. The CEB25-L111(T) array is stable in WT cells, it becomes unstable upon addition of Phen-DC3 Trifluoromethanesulfonate or deletion of PIF1. It is also highly destabilized in the presence of Phen-DC3 Trifluoromethanesulfonate or in the absence of PIF1. The CEB1-loop CEB25 allele remaines fully stable in both PIF1-treated and WT cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Phen-DC3 Trifluoromethanesulfonate Related Antibodies
Molecular Weight

848.75

Formula

C36H26F6N6O8S2
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

[O-]S(=O)(C(F)(F)F)=O.C[N+]1=C2C=CC=CC2=CC(NC(C3=CC=C4C=CC5=CC=C(C(NC6=C[N+](C)=C7C=CC=CC7=C6)=O)N=C5C4=N3)=O)=C1.[O-]S(=O)(C(F)(F)F)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 34 mg/mL (40.06 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1782 mL 5.8910 mL 11.7820 mL
5 mM 0.2356 mL 1.1782 mL 2.3564 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Volume (start)

V1

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.99%

COA (260 KB) HNMR (417 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[2]

Briefly, untreated WT cells and pif1Δ cells from a fresh patch of cells get from a single colony bearing the parental allele size are diluted in 5 mL of YPD (2×105 cells/mL), grown for 8 generations at 30°C with shaking, and spreaded as single colony on YPD plates. To measure minisatellite instability upon Phen-DC3 Trifluoromethanesulfonate treatment, WT cells from a fresh patch on YPD are grown for 8 generations at 30°C in liquid SC containing Phen-DC3 Trifluoromethanesulfonate at 10 μM. Isolated colonies or pools of colonies are analyzed by Southern blot using the EcoRI digestion that cuts at each side of the minisatellite[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1782 mL 5.8910 mL 11.7820 mL 29.4551 mL
5 mM 0.2356 mL 1.1782 mL 2.3564 mL 5.8910 mL
10 mM 0.1178 mL 0.5891 mL 1.1782 mL 2.9455 mL
15 mM 0.0785 mL 0.3927 mL 0.7855 mL 1.9637 mL
20 mM 0.0589 mL 0.2946 mL 0.5891 mL 1.4728 mL
25 mM 0.0471 mL 0.2356 mL 0.4713 mL 1.1782 mL
30 mM 0.0393 mL 0.1964 mL 0.3927 mL 0.9818 mL
40 mM 0.0295 mL 0.1473 mL 0.2946 mL 0.7364 mL
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Phen-DC3 Trifluoromethanesulfonate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phen-DC3929895-45-4Phen-DC3 TriflateG-quadruplexInhibitorinhibitorinhibit

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