Phen-DC3 Trifluoromethanesulfonate (Synonyms: Phen-DC3 Triflate)

Name: Phen-DC3 Trifluoromethanesulfonate
Brand: MedChemExpress
SKU: HY-15594A
Rating: 5.0 (9 reviews)

Cat. No.: HY-15594A Purity: 98.64%: Data Sheet
COA

SDS
Handling Instructions
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Phen-DC3 Trifluoromethanesulfonate is a G-quadruplex (G4) specific ligand which can inhibit FANCJ and DinG helicases with IC₅₀s of 65±6 and 50±10 nM, respectively.

For research use only. We do not sell to patients.

CAS No. : 929895-45-4

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 9 publication(s) in Google Scholar

Other Forms of Phen-DC3 Trifluoromethanesulfonate:

Phen-DC3 Get quote

9 Publications Citing Use of MCE Phen-DC3 Trifluoromethanesulfonate

RT-PCR

Bio/Physico-chemical Assay

Cell Proliferation/Viability Assay

Flow Cytometry

: Phen-DC3 Trifluoromethanesulfonate purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jun 20;319(Pt 1):145405. [Abstract]; FRET-MC profiles were obtained under different experimental conditions: (1) 0.2 μM F21T alone (black line), (2) 0.2 μM F21T with 0.4 μM Phen-DC3 Trifluoromethanesulfonate (PhenDC3) (red line), (3) 0.2 μM F21T with 0.4 μM PhenDC3 and 2 μM SOST RG4wt competitors (blue line), and (4) 0.2 μM F21T with 0.4 μM PhenDC3 and 2 μM SOST RG4mut competitors (purple line). The FRET-MC buffer was composed of 10 mM KCl, 90 mM LiCl, and 10 mM lithium cacodylate at pH 7.2.

: Phen-DC3 Trifluoromethanesulfonate purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105312.; Cell viability was analyzed using a CCK-8 kit under the G4 stabilizer treatment for 12 h, 24 h, and 48 h. Phen-DC3 Trifluoromethanesulfonate (Phen-DC3) (5-100 nM)-induced G4 stabilization inhibited NP cell viability in a dose-dependent manner.

: Phen-DC3 Trifluoromethanesulfonate purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105312.; mRNA expression of Il6 after the single or combined treatment of PDS, Braco-19, Phen-DC3 Trifluoromethanesulfonate (Phen-DC3) (5-20 nM) and IL-1β (10 ng/mL). Phen-DC3 promoted Il6 mRNA expression in NP cells in a dose-dependent manner by stabilizing genomic G4 structures.

: Phen-DC3 Trifluoromethanesulfonate purchased from MedChemExpress. Usage Cited in: iScience. 2022 Oct 9;25(11):105312.; Flow cytometry assay for the cell apoptosis under the treatment of PDS, Braco-19, and Phen-DC3 Trifluoromethanesulfonate (Phen-DC3) (5-20 nM).

: Phen-DC3 Trifluoromethanesulfonate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2021 Oct 25;12(11):999. [Abstract]; The relative enrichment of G-quadruplexes (G4) in Lhcgr CGI-II from TM3 cells treated with vehicle or 10 μM 5-ALA, 5 μM 360 A iodide, or 10 μM Phen-DC3 Trifluoromethanesulfonate (Phen-DC3) (48 h) by ChIP-qPCR analysis. The G-quadruplex antibody (BG4) or IgG were used, respectively. N = 3 for each group.

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Description

Phen-DC3 Trifluoromethanesulfonate is a G-quadruplex (G4) specific ligand which can inhibit FANCJ and DinG helicases with IC₅₀s of 65±6 and 50±10 nM, respectively.

IC₅₀ & Target

IC50: 65±6 nM (G4 substrate, FANCJ helicase), 50±10 nM (G4 substrate, DinG helicases)^[1]

In Vitro

In WT cells, a CEB1-WT array is rather stable but undergoes frequent rearrangements upon addition of 10 μM Phen-DC3 Trifluoromethanesulfonate (Phen-DC3). It is found that the c-Myc allele exhibits significant destabilization upon Phen-DC3 Trifluoromethanesulfonate treatment and PIF1 deletion. The CEB25-L111(T) array is stable in WT cells, it becomes unstable upon addition of Phen-DC3 Trifluoromethanesulfonate or deletion of PIF1. It is also highly destabilized in the presence of Phen-DC3 Trifluoromethanesulfonate or in the absence of PIF1. The CEB1-loop CEB25 allele remaines fully stable in both PIF1-treated and WT cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

848.75

Formula

C₃₆H₂₆F₆N₆O₈S₂

CAS No.

929895-45-4

Appearance

Solid

Color

White to light yellow

SMILES

[O-]S(=O)(C(F)(F)F)=O.C[N+]1=C2C=CC=CC2=CC(NC(C3=CC=C4C=CC5=CC=C(C(NC6=C[N+](C)=C7C=CC=CC7=C6)=O)N=C5C4=N3)=O)=C1.[O-]S(=O)(C(F)(F)F)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : ≥ 34 mg/mL (40.06 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1782 mL	5.8910 mL	11.7820 mL
5 mM	0.2356 mL	1.1782 mL	2.3564 mL
10 mM	0.1178 mL	0.5891 mL	1.1782 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.64%

Select Batch:

Data Sheet (283 KB) SDS (252 KB)

COA (260 KB) HNMR (421 KB) RP-HPLC (250 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Sanjay Kumar Bharti, et al. Specialization among Iron-Sulfur Cluster Helicases to Resolve G-quadruplex DNA Structures That Threaten Genomic Stability. J Biol Chem. 2013 Sep 27; 288(39): 28217–28229. [Content Brief]

[2]. Aurèle Piazza, et al. Short loop length and high thermal stability determine genomic instability induced by G-quadruplex-forming minisatellites. EMBO J. 2015 Jun 12; 34(12): 1718–1734. [Content Brief]

Cell Assay
^[2]

Briefly, untreated WT cells and pif1Δ cells from a fresh patch of cells get from a single colony bearing the parental allele size are diluted in 5 mL of YPD (2×10⁵ cells/mL), grown for 8 generations at 30°C with shaking, and spreaded as single colony on YPD plates. To measure minisatellite instability upon Phen-DC3 Trifluoromethanesulfonate treatment, WT cells from a fresh patch on YPD are grown for 8 generations at 30°C in liquid SC containing Phen-DC3 Trifluoromethanesulfonate at 10 μM. Isolated colonies or pools of colonies are analyzed by Southern blot using the EcoRI digestion that cuts at each side of the minisatellite^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Sanjay Kumar Bharti, et al. Specialization among Iron-Sulfur Cluster Helicases to Resolve G-quadruplex DNA Structures That Threaten Genomic Stability. J Biol Chem. 2013 Sep 27; 288(39): 28217–28229. [Content Brief]

[2]. Aurèle Piazza, et al. Short loop length and high thermal stability determine genomic instability induced by G-quadruplex-forming minisatellites. EMBO J. 2015 Jun 12; 34(12): 1718–1734. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1782 mL	5.8910 mL	11.7820 mL	29.4551 mL
	5 mM	0.2356 mL	1.1782 mL	2.3564 mL	5.8910 mL
	10 mM	0.1178 mL	0.5891 mL	1.1782 mL	2.9455 mL
	15 mM	0.0785 mL	0.3927 mL	0.7855 mL	1.9637 mL
	20 mM	0.0589 mL	0.2946 mL	0.5891 mL	1.4728 mL
	25 mM	0.0471 mL	0.2356 mL	0.4713 mL	1.1782 mL
	30 mM	0.0393 mL	0.1964 mL	0.3927 mL	0.9818 mL
	40 mM	0.0295 mL	0.1473 mL	0.2946 mL	0.7364 mL

Phen-DC3 Trifluoromethanesulfonate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phen-DC3929895-45-4Phen-DC3 TriflateG-quadruplexInhibitorinhibitorinhibit

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Phen-DC3 Trifluoromethanesulfonate (Synonyms: Phen-DC3 Triflate)

Customer Review

Other Forms of Phen-DC3 Trifluoromethanesulfonate:

Phen-DC3 Trifluoromethanesulfonate Related Antibodies

9 Publications Citing Use of MCE Phen-DC3 Trifluoromethanesulfonate

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Phen-DC3 Trifluoromethanesulfonate Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Phen-DC3 Trifluoromethanesulfonate Related Classifications

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