2. MAPK/ERK Pathway Apoptosis Cell Cycle/DNA Damage
  3. Mixed Lineage Kinase RIP kinase CDK
  4. NecroIr1

NecroIr1 is an iridium(III) complex, serves as necroptosis inducers in Cisplatin (HY-17394)-resistant lung cancer cells (A549R). NecroIr1 selectively accumulates in mitochondria, leading to oxidative stress and loss of mitochondrial membrane potential (MMP). NecroIr1 activates receptor-interacting serine-threonine kinase 3 (RIPK3) and Mixed Lineage Kinase (MLKL), and regulates CDK4 expression.

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NecroIr1 Chemical Structure

NecroIr1 Chemical Structure

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 725 In-stock
Solution
10 mM * 1 mL in DMSO USD 725 In-stock
Solid
5 mg USD 450 In-stock
10 mg USD 800 In-stock
25 mg USD 1650 In-stock
50 mg USD 2550 In-stock
100 mg USD 3800 In-stock
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Based on 1 publication(s) in Google Scholar

NecroIr1 Related Antibodies

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CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85

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Description

NecroIr1 is an iridium(III) complex, serves as necroptosis inducers in Cisplatin (HY-17394)-resistant lung cancer cells (A549R). NecroIr1 selectively accumulates in mitochondria, leading to oxidative stress and loss of mitochondrial membrane potential (MMP). NecroIr1 activates receptor-interacting serine-threonine kinase 3 (RIPK3) and Mixed Lineage Kinase (MLKL), and regulates CDK4 expression[1].

IC50 & Target[1]

CDK4

 

RIPK3

 

RIPK1

 

In Vitro

NecroIr1 (2 μM; 1-2 d) exerts subcellular distribution over 90% accumulated in mitochondria in non-labelled A549R and CFSElabelled L02 cells[1].
NecroIr1 (1.5 μM and 3 μM; 24 h) results ROS generation increasing and loss of mitochondrial membrane potential[1].
NecroIr1 (1.5 μM and 3 μM; 24 h) activates necroptosis proteins, increases the phosphorylation of RIPK1 and RIPK3[1].
NecroIr1 (0.75 μM and 1.5 μM; 24 h) induces necroptosis by arresting cell cycle at G0/G1[1].
NecroIr1 (0.75-3.0 μM; 24 h) inhibits A549R cells proliferation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NecroIr1 Related Antibodies

Western Blot Analysis[1]

Cell Line: A549R cells
Concentration: 1.5 μM and 3 μM
Incubation Time: 24 hours
Result: Increased phospho-RIPK1 (p-PIPK1), total RIPK3, and phospho-RIPK3 (p-PIPK3) level.

Cell Cycle Analysis[1]

Cell Line: A549R cells
Concentration: 0 μM, 0.75 μM and 1.5 μM
Incubation Time: 24 hours
Result: Arrested cell cycle at G0/G1 phase in a dose-dependent manner.

Cell Proliferation Assay[1]

Cell Line: A549R cells
Concentration: 0 μM, 0.75 μM, 1.5 μM and 3.0 μM
Incubation Time: 24 hours
Result: Inhibits cell proliferation in a dose-dependent manner.
Molecular Weight

823.36

Formula

C40H29ClIrN5O-
Appearance

Solid

Color

Orange to red

SMILES

CC1=CC(C2=[N]3C=CC(C4=NC(C=CC=C5)=C5O4)=C2)=[N]([Ir]67([N]8=CC=CC=C8C9=C7C=CC=C9)3[N]%10=CC=CC=C%10C%11=C6C=CC=C%11)C=C1.[+].[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (121.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2145 mL 6.0727 mL 12.1454 mL
5 mM 0.2429 mL 1.2145 mL 2.4291 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2145 mL 6.0727 mL 12.1454 mL 30.3634 mL
5 mM 0.2429 mL 1.2145 mL 2.4291 mL 6.0727 mL
10 mM 0.1215 mL 0.6073 mL 1.2145 mL 3.0363 mL
15 mM 0.0810 mL 0.4048 mL 0.8097 mL 2.0242 mL
20 mM 0.0607 mL 0.3036 mL 0.6073 mL 1.5182 mL
25 mM 0.0486 mL 0.2429 mL 0.4858 mL 1.2145 mL
30 mM 0.0405 mL 0.2024 mL 0.4048 mL 1.0121 mL
40 mM 0.0304 mL 0.1518 mL 0.3036 mL 0.7591 mL
50 mM 0.0243 mL 0.1215 mL 0.2429 mL 0.6073 mL
60 mM 0.0202 mL 0.1012 mL 0.2024 mL 0.5061 mL
80 mM 0.0152 mL 0.0759 mL 0.1518 mL 0.3795 mL
100 mM 0.0121 mL 0.0607 mL 0.1215 mL 0.3036 mL
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NecroIr1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NecroIr1NecroIr 1NecroIr-1Mixed Lineage KinaseRIP kinaseCDKMLKsReceptor-interacting protein kinasesRIPKCyclin dependent kinaseCisplatinCisplatin-resistant lung cancer cellsmitochondriamitochondrial membrane potentialMMPnecroptosisInhibitorinhibitorinhibit

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