2. MAPK/ERK Pathway Apoptosis Cell Cycle/DNA Damage
  3. Mixed Lineage Kinase RIP kinase CDK
  4. NecroIr2

NecroIr2 is an iridium(III) complex, serves as necroptosis inducers in Cisplatin (HY-17394)-resistant lung cancer cells (A549R). NecroIr2 selectively accumulates in mitochondria, leading to oxidative stress and loss of mitochondrial membrane potential (MMP). NecroIr2 activates receptor-interacting serine-threonine kinase 3 (RIPK3) and mixed lineage kinase domain-like pseudokinase (MLKL), and regulates CDK4 expression.

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NecroIr2 Chemical Structure

NecroIr2 Chemical Structure

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 693 In-stock
Solution
10 mM * 1 mL in DMSO USD 693 In-stock
Solid
5 mg USD 400 In-stock
10 mg USD 680 In-stock
25 mg USD 1350 In-stock
50 mg USD 2200 In-stock
100 mg USD 3500 In-stock
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Based on 1 publication(s) in Google Scholar

NecroIr2 Related Antibodies

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CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85

  • Biological Activity

  • Purity & Documentation

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Description

NecroIr2 is an iridium(III) complex, serves as necroptosis inducers in Cisplatin (HY-17394)-resistant lung cancer cells (A549R). NecroIr2 selectively accumulates in mitochondria, leading to oxidative stress and loss of mitochondrial membrane potential (MMP). NecroIr2 activates receptor-interacting serine-threonine kinase 3 (RIPK3) and mixed lineage kinase domain-like pseudokinase (MLKL), and regulates CDK4 expression[1].

IC50 & Target[1]

CDK4

 

RIPK3

 

RIPK1

 

In Vitro

NecroIr2 (2 μM; 1-2 d) exerts subcellular distribution over 90% accumulated in mitochondria in non-labelled A549R and CFSE labelled L02 cells[1].
NecroIr2 (1.5 μM and 3 μM; 24 h) results ROS generation increasing and loss of mitochondrial membrane potential[1].
NecroIr2 (1.5 μM and 3 μM; 24 h) activates necroptosis proteins, increases the phosphorylation of RIPK1 and RIPK3[1].
NecroIr2 (0.75 μM and 1.5 μM; 24 h) induces necroptosis by arresting cell cycle at G0/G1[1].
NecroIr2 (0.375-1.5 μM; 24 h) inhibits A549R cells proliferation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NecroIr2 Related Antibodies

Western Blot Analysis[1]

Cell Line: A549R cells
Concentration: 1.5 μM and 3 μM
Incubation Time: 24 hours
Result: Increased phospho-RIPK1 (p-PIPK1), total RIPK3, and phospho-RIPK3 (p-PIPK3) level.

Cell Cycle Analysis[1]

Cell Line: A549R cells
Concentration: 0 μM, 0.75 μM and 1.5 μM
Incubation Time: 24 hours
Result: Arrested cell cycle at G0/G1 phase in a dose-dependent manner.

Cell Proliferation Assay[1]

Cell Line: A549R cells
Concentration: 0 μM, 0.375 μM, 0.75 μM and 1.5 μM
Incubation Time: 24 hours
Result: Inhibits cell proliferation in a dose-dependent manner.
Molecular Weight

926.44

Formula

C46H30ClIrN6O2-
Appearance

Solid

Color

Brown to reddish brown

SMILES

C1(C2=NC(C=CC=C3)=C3O2)=CC(C4=[N]5C=CC(C6=NC(C=CC=C7)=C7O6)=C4)=[N]([Ir]89([N]%10=CC=CC=C%10C%11=C9C=CC=C%11)5[N]%12=CC=CC=C%12C%13=C8C=CC=C%13)C=C1.[Cl-].[+]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (53.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0794 mL 5.3970 mL 10.7940 mL
5 mM 0.2159 mL 1.0794 mL 2.1588 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.0794 mL 5.3970 mL 10.7940 mL 26.9850 mL
5 mM 0.2159 mL 1.0794 mL 2.1588 mL 5.3970 mL
10 mM 0.1079 mL 0.5397 mL 1.0794 mL 2.6985 mL
15 mM 0.0720 mL 0.3598 mL 0.7196 mL 1.7990 mL
20 mM 0.0540 mL 0.2699 mL 0.5397 mL 1.3493 mL
25 mM 0.0432 mL 0.2159 mL 0.4318 mL 1.0794 mL
30 mM 0.0360 mL 0.1799 mL 0.3598 mL 0.8995 mL
40 mM 0.0270 mL 0.1349 mL 0.2699 mL 0.6746 mL
50 mM 0.0216 mL 0.1079 mL 0.2159 mL 0.5397 mL
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NecroIr2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NecroIr2NecroIr 2NecroIr-2Mixed Lineage KinaseRIP kinaseCDKMLKsReceptor-interacting protein kinasesRIPKCyclin dependent kinaseCisplatinCisplatin-resistant lung cancer cellsmitochondriamitochondrial membrane potentialMMPnecroptosisInhibitorinhibitorinhibit

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