Cat. No.: HY-148366

NecroIr2

Customer Review

Based on 1 Customer Validation

NecroIr2 is an iridium(III) complex, serves as necroptosis inducers in Cisplatin (HY-17394)-resistant lung cancer cells (A549R). NecroIr2 selectively accumulates in mitochondria, leading to oxidative stress and loss of mitochondrial membrane potential (MMP). NecroIr2 activates receptor-interacting serine-threonine kinase 3 (RIPK3) and mixed lineage kinase domain-like pseudokinase (MLKL), and regulates CDK4 expression.

For research use only. We do not sell to patients.

Purity: 98.02%
Formula: C₄₆H₃₀ClIrN₆O₂^-
Molecular Weight:926.44

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

NecroIr2 Chemical Structure

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

CDK4

RIPK3

RIPK1

All RIP kinase Isoforms :

All CDK Isoforms :

In Vitro

NecroIr2 (2 μM; 1-2 d) exerts subcellular distribution over 90% accumulated in mitochondria in non-labelled A549R and CFSE labelled L02 cells^[1].
NecroIr2 (1.5 μM and 3 μM; 24 h) results ROS generation increasing and loss of mitochondrial membrane potential^[1].
NecroIr2 (1.5 μM and 3 μM; 24 h) activates necroptosis proteins, increases the phosphorylation of RIPK1 and RIPK3^[1].
NecroIr2 (0.75 μM and 1.5 μM; 24 h) induces necroptosis by arresting cell cycle at G0/G1^[1].
NecroIr2 (0.375-1.5 μM; 24 h) inhibits A549R cells proliferation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

A549R cells
Concentration:

1.5 μM and 3 μM
Incubation Time:

24 hours
Result:

Increased phospho-RIPK1 (p-PIPK1), total RIPK3, and phospho-RIPK3 (p-PIPK3) level.

Cell Cycle Analysis^[1]

Cell Line:

A549R cells
Concentration:

0 μM, 0.75 μM and 1.5 μM
Incubation Time:

24 hours
Result:

Arrested cell cycle at G0/G1 phase in a dose-dependent manner.

Cell Proliferation Assay^[1]

Cell Line:

A549R cells
Concentration:

0 μM, 0.375 μM, 0.75 μM and 1.5 μM
Incubation Time:

24 hours
Result:

Inhibits cell proliferation in a dose-dependent manner.

Chemical Information

Appearance Solid
Molecular Weight 926.44
Formula C₄₆H₃₀ClIrN₆O₂^-
Color Brown to reddish brown
SMILES

C1(C2=NC(C=CC=C3)=C3O2)=CC(C4=[N]5C=CC(C6=NC(C=CC=C7)=C7O6)=C4)=[N]([Ir]89([N]%10=CC=CC=C%10C%11=C9C=CC=C%11)5[N]%12=CC=CC=C%12C%13=C8C=CC=C%13)C=C1.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (53.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.0794 mL

5.3970 mL

10.7940 mL

5 mM

0.2159 mL

1.0794 mL

2.1588 mL

10 mM

0.1079 mL

0.5397 mL

1.0794 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Guan R, et al. Necroptosis-inducing iridium (III) complexes as regulators of cyclin-dependent kinases[J]. Inorganic Chemistry Frontiers, 2021, 8(7): 1788-1794.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.0794 mL	5.3970 mL	10.7940 mL	26.9850 mL
	5 mM	0.2159 mL	1.0794 mL	2.1588 mL	5.3970 mL
	10 mM	0.1079 mL	0.5397 mL	1.0794 mL	2.6985 mL
	15 mM	0.0720 mL	0.3598 mL	0.7196 mL	1.7990 mL
	20 mM	0.0540 mL	0.2699 mL	0.5397 mL	1.3493 mL
	25 mM	0.0432 mL	0.2159 mL	0.4318 mL	1.0794 mL
	30 mM	0.0360 mL	0.1799 mL	0.3598 mL	0.8995 mL
	40 mM	0.0270 mL	0.1349 mL	0.2699 mL	0.6746 mL
	50 mM	0.0216 mL	0.1079 mL	0.2159 mL	0.5397 mL

Keywords

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NecroIr2

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

MAPK Compound Library

NF-κB Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Oxygen Sensing Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Protein-protein Interaction Inhibitor Library

Cancer Stem Cells Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Serine/Threonine Kinase Inhibitor Library

Anti-Drug-Resistant Compound Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Mitophagy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Protein Kinase Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

High-Efficiency Gene Editing Compound Library

All RIP kinase Isoforms

All CDK Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

NecroIr2 Related Classifications

Cat. No.: HY-148366