WDFY3 Antibody (YA4364)

Cat. No.: HY-P84667: Data Sheet User Guide for Antibodies
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WDFY3 Antibody (YA4364) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to WDFY3.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

WDFY3 Antibody (YA4364) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to WDFY3.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 395 kDa;

Observed band size: 395 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q8IZQ1

Gene ID

23001 [NCBI]

Immunogen

Purified recombinant fragment of human WDFY3 (AA: 3277-3526) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:It was expressed in all the tissues tested.

Isoforms & Post-Translational Modification：Q15653 has 2 isomers: Q15653-1: 37771 Da (predicted); Q15653-2: 35530 Da (predicted).
Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation

Subunit：Interacts with THRB (via ligand-binding domain) (PubMed:7776974). Interacts with RELA and REL (By similarity). Interacts with COMMD1 (PubMed:16573520).

Synonyms

ALFY; BCHS; MCPH18; ZFYVE25

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/ccrf-cem-cell.html [Content Brief]