Art. -Nr.: HY-157762

GPX4-IN-9

Name: GPX4-IN-9
Brand: MedChemExpress
SKU: HY-157762
Price: 465.00 USD
Availability: InStock
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GPX4-IN-9 (Compound A16) is a glutathione peroxidase 4 (GPX4) inhibitor that specifically targets GPX4 under both in vitro and in vivo conditions, inducing ferroptosis. GPX4-IN-9 exhibits cytotoxicity against pancreatic cancer cells and can be used in cancer research.

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Reinheit : 99.18%
CAS. Nr.: 3032746-99-6
Formel: C₂₆H₂₁N₃O₂S₂
Molecular Weight:471.59

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

GPX4-IN-9 Chemical Structure

CAS. Nr.: 3032746-99-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

GPX4

Alle Glutathione Peroxidase Isoform-spezifische Produkte anzeigen :

GPX1
GPX4

Cellular Effect

Cell Line	Type	Value	Description	References
ASPC1	IC₅₀	0.022 μM Compound: A16	Antiproliferative activity against human AsPC1 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay Antiproliferative activity against human AsPC1 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay	[PMID: 38265413]
ASPC1	IC₅₀	14 nM Compound: A16	Induction of ferroptosis in human ASPC1 cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay Induction of ferroptosis in human ASPC1 cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay	[PMID: 38265413]
ASPC1	IC₅₀	26 nM Compound: A16	Induction of ferroptosis in human ASPC1 cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay Induction of ferroptosis in human ASPC1 cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay	[PMID: 38265413]
ASPC1	IC₅₀	29 nM Compound: A16	Induction of ferroptosis in human ASPC1 cells assessed as reduction in cell viability incubated for 72 hrs followed by compound washout by CCK-8 assay Induction of ferroptosis in human ASPC1 cells assessed as reduction in cell viability incubated for 72 hrs followed by compound washout by CCK-8 assay	[PMID: 38265413]
ASPC1	IC₅₀	33 nM Compound: A16	Induction of ferroptosis in human ASPC1 cells assessed as reduction in cell viability incubated for 48 hrs followed by compound washout by CCK-8 assay Induction of ferroptosis in human ASPC1 cells assessed as reduction in cell viability incubated for 48 hrs followed by compound washout by CCK-8 assay	[PMID: 38265413]
ASPC1	IC₅₀	37 nM Compound: A16	Induction of ferroptosis in human ASPC1 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Induction of ferroptosis in human ASPC1 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 38265413]
ASPC1	IC₅₀	62 nM Compound: A16	Induction of ferroptosis in human ASPC1 cells assessed as reduction in cell viability incubated for 24 hrs followed by compound washout by CCK-8 assay Induction of ferroptosis in human ASPC1 cells assessed as reduction in cell viability incubated for 24 hrs followed by compound washout by CCK-8 assay	[PMID: 38265413]
BXPC-3	IC₅₀	0.02 μM Compound: A16	Antiproliferative activity against human BXPC-3 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay Antiproliferative activity against human BXPC-3 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay	[PMID: 38265413]
CFPAC-1	IC₅₀	0.02 μM Compound: A16	Antiproliferative activity against human CFPAC-1 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay Antiproliferative activity against human CFPAC-1 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay	[PMID: 38265413]
HCT-116	IC₅₀	>10 nM Compound: A16	Induction of ferroptosis in human HCT-116 cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay Induction of ferroptosis in human HCT-116 cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay	[PMID: 38265413]
HPAF-II	IC₅₀	0.02 μM Compound: A16	Antiproliferative activity against human HPAF-II cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay Antiproliferative activity against human HPAF-II cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay	[PMID: 38265413]
MIA PaCa-2	IC₅₀	0.02 μM Compound: A16	Antiproliferative activity against human MIA PaCa-2 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay Antiproliferative activity against human MIA PaCa-2 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay	[PMID: 38265413]
NCI-H522	IC₅₀	3 nM Compound: A16	Induction of ferroptosis in human NCI-H522 cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay Induction of ferroptosis in human NCI-H522 cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay	[PMID: 38265413]
PANC-1	IC₅₀	0.02 μM Compound: A16	Antiproliferative activity against human PANC-1 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay Antiproliferative activity against human PANC-1 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay	[PMID: 38265413]
SW1990	IC₅₀	0.02 μM Compound: A16	Antiproliferative activity against human SW1990 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay Antiproliferative activity against human SW1990 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay	[PMID: 38265413]

Chemical Information

CAS. Nr. 3032746-99-6
Appearance Solid
Molecular Weight 471.59
Formel C₂₆H₂₁N₃O₂S₂
Color Off-white to light yellow
SMILES

O=S(C(C=C1)=CC=C1CN(C2=CC=C(C=C2)C3=NC4=C(S3)C=CC=C4)CC#C)(N(C#C)C)=O
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (106.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1205 mL

10.6024 mL

21.2049 mL

5 mM

0.4241 mL

2.1205 mL

4.2410 mL

10 mM

0.2120 mL

1.0602 mL

2.1205 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Tang Z, et al. Novel Covalent Probe Selectively Targeting Glutathione Peroxidase 4 In Vivo: Potential Applications in PancrTang Z, et al. Novel Covalent Probe Selectively Targeting Glutathione Peroxidase 4 In Vivo: Potential Applications in Pancreatic Cancer Therapy. J Med Chem. 2024 Feb 8;67(3):1872-1887.eatic Cancer Therapy. J Med Chem. 2024 Feb 8;67(3):1872-1887. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1205 mL	10.6024 mL	21.2049 mL	53.0122 mL
	5 mM	0.4241 mL	2.1205 mL	4.2410 mL	10.6024 mL
	10 mM	0.2120 mL	1.0602 mL	2.1205 mL	5.3012 mL
	15 mM	0.1414 mL	0.7068 mL	1.4137 mL	3.5341 mL
	20 mM	0.1060 mL	0.5301 mL	1.0602 mL	2.6506 mL
	25 mM	0.0848 mL	0.4241 mL	0.8482 mL	2.1205 mL
	30 mM	0.0707 mL	0.3534 mL	0.7068 mL	1.7671 mL
	40 mM	0.0530 mL	0.2651 mL	0.5301 mL	1.3253 mL
	50 mM	0.0424 mL	0.2120 mL	0.4241 mL	1.0602 mL
	60 mM	0.0353 mL	0.1767 mL	0.3534 mL	0.8835 mL
	80 mM	0.0265 mL	0.1325 mL	0.2651 mL	0.6627 mL
	100 mM	0.0212 mL	0.1060 mL	0.2120 mL	0.5301 mL

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GPX4-IN-9

Alle Glutathione Peroxidase Isoform-spezifische Produkte anzeigen