SR18662
Based on 1 publication(s) in Google Scholar
SR18662 is a potent inhibitor of Krüppel-like factor five (KLF5) with an IC50 of 4.4 nM and an analogue of ML264 (HY-19994) with improved inhibitory potency against colorectal cancer cells. SR18662 can be used for the study of colorectal cancer.
For research use only. We do not sell to patients.
- Purity: 99.61%
- CAS No.: 2505001-62-5
- Formula: C16H19Cl2N3O4S
- Molecular Weight:420.31
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Immunology/Inflammation Compound Library
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Publications Citing Use of MedChemExpress (MCE) SR18662
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Biological Activity
IC50: 4.4 nM (KLF5)[1]
SR18662 (0-10 μM; 24-72 hours) significantly reduces growth and proliferation of CRC cells as compared to treatment with vehicle control, ML264 (HY-19994). It shows improved efficacy in reducing viability of multiple CRC cell lines[1].SR18662 (10 μM; 24-72 hours) shows a significant increase in the number of apoptotic cells at both early and late states in DLD-1 and HCT116 cells[1].SR18662 (1 μM; 72 hours) reduces the expression of cyclins (cyclins E, A2, and B1) and components of MAPK (p-Erk) and WNT signaling pathways (p-GSK3 β) in cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:CRC cells
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Concentration:0-10 μM
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Incubation Time:24 hours, 48 hours, 72 hours
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Result:Induced anti-tumor activity in colorectal cancer cell lines.
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Cell Line:DLD-1 and HCT116 cells
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Concentration:10 μM
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Incubation Time:24 hours, 48 hours, 72 hours
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Result:Increased apoptosis of colorectal cancer cell lines.
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Cell Line:DLD-1 and HCT116 cells
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Concentration:1 μM
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Incubation Time:72 hours
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Result:Reduced levels of cyclins E, A2, and B1 inhibits activity of MAPK, WNT/β-catenin signaling pathways and decreases the levels of cyclins.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Nude mice with DLD-1 cells[1]
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Dosage:5 mg/kg; 10 mg/kg; 25 mg/kg
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Administration:Intraperitoneal injection; 5mg/kg daily, 5mg/kg twice a day,10 mg/kg daily, 10 mg/kg twice per day, 25mg/kg daily, and 25 mg/kg twice per day; 5 days of injections, 2 days break, and 5 days of injections
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Result:Caused a significant dose-dependent inhibition of xenograft growth in mice.
Chemical Information
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CAS No. 2505001-62-5
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Appearance Solid
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Molecular Weight 420.31
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Formula C16H19Cl2N3O4S
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Color White to off-white
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SMILES
ClC1=CC(/C=C/C(NCC(N2CCN(CC2)S(C)(=O)=O)=O)=O)=CC=C1Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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Nature
2026 Jun;654(8117):261-271. PMID: 41986722
Solvent & Solubility
DMSO : 125 mg/mL (297.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (4.95 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.08 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3792 mL | 11.8960 mL | 23.7920 mL | 59.4799 mL |
| 5 mM | 0.4758 mL | 2.3792 mL | 4.7584 mL | 11.8960 mL | |
| 10 mM | 0.2379 mL | 1.1896 mL | 2.3792 mL | 5.9480 mL | |
| 15 mM | 0.1586 mL | 0.7931 mL | 1.5861 mL | 3.9653 mL | |
| 20 mM | 0.1190 mL | 0.5948 mL | 1.1896 mL | 2.9740 mL | |
| 25 mM | 0.0952 mL | 0.4758 mL | 0.9517 mL | 2.3792 mL | |
| 30 mM | 0.0793 mL | 0.3965 mL | 0.7931 mL | 1.9827 mL | |
| 40 mM | 0.0595 mL | 0.2974 mL | 0.5948 mL | 1.4870 mL | |
| 50 mM | 0.0476 mL | 0.2379 mL | 0.4758 mL | 1.1896 mL | |
| 60 mM | 0.0397 mL | 0.1983 mL | 0.3965 mL | 0.9913 mL | |
| 80 mM | 0.0297 mL | 0.1487 mL | 0.2974 mL | 0.7435 mL | |
| 100 mM | 0.0238 mL | 0.1190 mL | 0.2379 mL | 0.5948 mL |
SR18662 Related Classifications
HY-136530 Related Classifications
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