Epicornuin F
Epicornuin F is a prenylated flavonoid. Epicornuin F can be isolated from the leaves of Epimedium brevicornu. Epicornuin F has the cytotoxic activity against HepG2 cells with an IC50 value of 33.4 μM. Epicornuin F can be used for the research of cancer.
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- CAS No.: 1812887-72-1
- Formule: C25H26O7
- Masse moléculaire:438.47
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Activité biologique
Description
IC50 & Target
IC50: 33.4 μM (HepG2 cells)[1]
In Vitro
Epicornuin F has the cytotoxic activity against HepG2 cells with an IC50 value of 33.4 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1812887-72-1
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Masse moléculaire 438.47
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Formule C25H26O7
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SMILES
O=C1C=C(C2=CC(CC(O)C(C)=C)=C(O)C(O)=C2)OC3=C(C/C=C(C)\C)C(O)=CC(O)=C13
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)