ICG-alkyne
Based on 1 Customer Validation
ICG-alkyne is a short-wave infrared (SWIR)-emitting cyanine fluorescent probe based on alkyne-derivatized indocyanine green. ICG-alkyne can undergo click chemistry reaction to conjugate with antibody molecules via binding to the azide groups introduced into antibodies. ICG-alkyne enables SWIR fluorescent molecular imaging of breast tumors in living mice. ICG-alkyne can be used in breast cancer-related research.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté : 98.27%
- CAS No.: 1622335-41-4
- Formule: C48H53N3O4S
- Masse moléculaire:768.02
-
Stockage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
-
Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
-
Help & FAQs
Activité biologique
Description
In Vitro
ICG-alkyne (1 mg/mL; 12 h) forms functional conjugates with Erbitux via click chemistry, enabling specific targeting and SWIR fluorescence visualization of EGFR on MDA-MB-468 human breast cancer cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
CAS No. 1622335-41-4
-
Appearance Solid
-
Masse moléculaire 768.02
-
Formule C48H53N3O4S
-
Color Brown to black
-
SMILES
O=C(CCCCCN1C2=C(C(C)(C)/C1=C\C=C\C=C\C=C\C3=[N+](CCCCS(=O)([O-])=O)C4=C(C3(C)C)C5=C(C=CC=C5)C=C4)C6=C(C=CC=C6)C=C2)NCC#C
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvant et solubilité
In Vitro:
DMSO : 200 mg/mL (260.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
-
EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
-
Breast Cancer Modeling
Breast cancer is a heterogeneous cancer, and it has been distinguished into four subtypes: luminal A, luminal B, HER2-positive and basal-like. Molecular mutations, epigenetic alterations, hormone exposure and immune microenvironment are related to the progression of breast cancer.
Pureté et documentation
-
Fiche technique (275 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3020 mL | 6.5102 mL | 13.0205 mL | 32.5512 mL |
| 5 mM | 0.2604 mL | 1.3020 mL | 2.6041 mL | 6.5102 mL | |
| 10 mM | 0.1302 mL | 0.6510 mL | 1.3020 mL | 3.2551 mL | |
| 15 mM | 0.0868 mL | 0.4340 mL | 0.8680 mL | 2.1701 mL | |
| 20 mM | 0.0651 mL | 0.3255 mL | 0.6510 mL | 1.6276 mL | |
| 25 mM | 0.0521 mL | 0.2604 mL | 0.5208 mL | 1.3020 mL | |
| 30 mM | 0.0434 mL | 0.2170 mL | 0.4340 mL | 1.0850 mL | |
| 40 mM | 0.0326 mL | 0.1628 mL | 0.3255 mL | 0.8138 mL | |
| 50 mM | 0.0260 mL | 0.1302 mL | 0.2604 mL | 0.6510 mL | |
| 60 mM | 0.0217 mL | 0.1085 mL | 0.2170 mL | 0.5425 mL | |
| 80 mM | 0.0163 mL | 0.0814 mL | 0.1628 mL | 0.4069 mL | |
| 100 mM | 0.0130 mL | 0.0651 mL | 0.1302 mL | 0.3255 mL |
ICG-alkyne Related Classifications
HY-151704 Related Classifications
Powered by Bioz