LIT-001 free base
LIT-001 free base is the first nonpeptide oxytocin receptor (OT-R) agonist (EC50=55 nM; Ki=226 nM). LIT-001 free base improves social interaction in a mouse model of autism.
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- CAS No.: 2245072-20-0
- Formule: C28H33N7O2S
- Masse moléculaire:531.67
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Stockage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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HY-17571A
Oxytocin acetateHypothalamic Peptide -
HY-15023
CligosibanOxytocin Receptor Antagonist -
HY-124733A
LIT-001Oxytocin Receptor Agonist -
HY-P4895
(d(CH2)51,Tyr(Me)2,Orn8)-OxytocinOxytocin Receptor Antagonist -
HY-15015
OT-R antagonist 1Oxytocin Receptor Antagonist -
HY-P2168
DemoxytocinOxytocin Analog -
HY-15023R
Cligosiban (Standard)Oxytocin Receptor Antagonist -
HY-14778
RetosibanOxytocin Receptor Antagonist -
HY-15008
L-368,899OT Receptor Antagonist -
HY-105018
Epelsiban -
HY-128351
SHR1653Oxytocin Receptor Antagonist -
HY-P3516
BarusibanOT-R Antagonist
Activité biologique
Description
In Vitro
In vitro signaling experiments, LIT-001 free base is a nonbiased OT-R agonist on the two main signaling pathways of this receptor, with minor antagonist effect on V1a and agonist effect on V1b receptors, observed at high concentrations only[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Oprm1–/– mice (bred in house on a 50% 129SVPas–50% C57BL/6J hybrid background)[1]
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Dosage:10, 20 mg/kg
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Administration:i.p.
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Result:Alleviated core symptoms in the context of ASD.
Chemical Information
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CAS No. 2245072-20-0
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Appearance Solid
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Masse moléculaire 531.67
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Formule C28H33N7O2S
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Color White to off-white
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SMILES
O=C(N1[C@H](C(N(C)C)=S)CCC1)NCC2=CC=C(C(N3CC4=C(N(C)N=C4)NC5=CC=CC=C35)=O)C=C2C
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (188.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (277 KB)
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SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.8809 mL | 9.4043 mL | 18.8087 mL | 47.0217 mL |
| 5 mM | 0.3762 mL | 1.8809 mL | 3.7617 mL | 9.4043 mL | |
| 10 mM | 0.1881 mL | 0.9404 mL | 1.8809 mL | 4.7022 mL | |
| 15 mM | 0.1254 mL | 0.6270 mL | 1.2539 mL | 3.1348 mL | |
| 20 mM | 0.0940 mL | 0.4702 mL | 0.9404 mL | 2.3511 mL | |
| 25 mM | 0.0752 mL | 0.3762 mL | 0.7523 mL | 1.8809 mL | |
| 30 mM | 0.0627 mL | 0.3135 mL | 0.6270 mL | 1.5674 mL | |
| 40 mM | 0.0470 mL | 0.2351 mL | 0.4702 mL | 1.1755 mL | |
| 50 mM | 0.0376 mL | 0.1881 mL | 0.3762 mL | 0.9404 mL | |
| 60 mM | 0.0313 mL | 0.1567 mL | 0.3135 mL | 0.7837 mL | |
| 80 mM | 0.0235 mL | 0.1176 mL | 0.2351 mL | 0.5878 mL | |
| 100 mM | 0.0188 mL | 0.0940 mL | 0.1881 mL | 0.4702 mL |
LIT-001 free base Related Classifications
HY-124733 Related Classifications
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