Glutathione ethyl ester
Based on 1 Customer Validation
Glutathione ethyl ester is a cell-permeable GSH donor and provides an efficient supply of GSH to the oocyte. Glutathione ethyl ester shows positive effect on the in vitro production of embryos by enhancement of the antioxidative defense.
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- Reinheit: 98.23%
- CAS. Nr.: 92614-59-0
- Formel: C12H21N3O6S
- Molecular Weight:335.38
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Speicherung:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Chemical Information
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Biologische Aktivität
IC50: GSH[1]
Glutathione ethyl ester (5 mM) reduces the intracellular reactive oxygen species (ROS) content, promotes meiotic progression, increases the proportion of oocytes with mature cytoplasm (evenly dispersed cortical granules), and improves the normal fertilization rate, blastocyst development rate, and the proportion of diploid blastocysts when treating oocytes from aged cows[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Swiss-Webster mice (age 2 mo, body wt 25-30 g), a model of exercise-induced oxidative stress[2].
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Dosage:6 mmol/kg (saline)
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Administration:Intraperitoneal injection, once, 1 h before the experiment.
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Result:Normalized endurance performance in starved mice.
Decreased muscle GSH content and the GSH:GSSG ratio.
Also significantly increased endurance time and suppressed exercise-induced muscle lipid peroxidation
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γGlu-Cys-Gly-ethyl ester
γGlu-CG-ethyl ester
Chemical Information
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CAS. Nr. 92614-59-0
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Appearance Solid
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Molecular Weight 335.38
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Formel C12H21N3O6S
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Color White to off-white
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (298.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (298.17 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (14.91 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (14.91 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Reinheit & Dokumentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. F Magata, et al. Glutathione ethyl ester improved the age-induced decline in the developmental competence of bovine oocytes. Theriogenology. 2021 Jun:167:37-43. [Content Brief]
[2]. Leeuwenburgh C, et al. Glutathone and glutathione ethyl ester supplementation of mice alter glutathione homeostasis during exercise. J Nutr. 1998 Dec;128(12):2420-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.9817 mL | 14.9085 mL | 29.8169 mL | 74.5423 mL |
| 5 mM | 0.5963 mL | 2.9817 mL | 5.9634 mL | 14.9085 mL | |
| 10 mM | 0.2982 mL | 1.4908 mL | 2.9817 mL | 7.4542 mL | |
| 15 mM | 0.1988 mL | 0.9939 mL | 1.9878 mL | 4.9695 mL | |
| 20 mM | 0.1491 mL | 0.7454 mL | 1.4908 mL | 3.7271 mL | |
| 25 mM | 0.1193 mL | 0.5963 mL | 1.1927 mL | 2.9817 mL | |
| 30 mM | 0.0994 mL | 0.4969 mL | 0.9939 mL | 2.4847 mL | |
| 40 mM | 0.0745 mL | 0.3727 mL | 0.7454 mL | 1.8636 mL | |
| 50 mM | 0.0596 mL | 0.2982 mL | 0.5963 mL | 1.4908 mL | |
| 60 mM | 0.0497 mL | 0.2485 mL | 0.4969 mL | 1.2424 mL | |
| 80 mM | 0.0373 mL | 0.1864 mL | 0.3727 mL | 0.9318 mL | |
| 100 mM | 0.0298 mL | 0.1491 mL | 0.2982 mL | 0.7454 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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