Ombitasvir
Based on 9 publication(s) in Google Scholar
Ombitasvir is a potent inhibitor of the hepatitis C virus protein NS5A, with EC50s of 0.82 to 19.3 pM against HCV genotypes 1 to 5, and 366 pM against genotype 6a.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté : 98.62%
- CAS No.: 1258226-87-7
- Formule: C50H67N7O8
- Masse moléculaire:894.11
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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HY-15789
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LedipasvirHCV NS5A Inhibitor -
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ParitaprevirNS3/4A Protease Inhibitor -
HY-10466
DaclatasvirHCV NS5A Inhibitor -
HY-101662
Pibrentasvir -
HY-17634
GlecaprevirHCV NS3/4A Protease Inhibitor -
HY-10465
Daclatasvir dihydrochlorideHCV NS5A Inhibitor -
HY-15005
Sofosbuvir -
HY-10237
BoceprevirHCV NS3 Protease Inhibitor -
HY-10999
TrametinibMEK Inhibitor -
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SimeprevirHCV NS3/4A Inhibitor
Publications Citing Use of MedChemExpress (MCE) Ombitasvir
More- Int J Mol Sci. 2025 Feb 6;26(3):1381. [Abstract]
- Antiviral Res. 2017 Mar:139:18-24. [Abstract]
- J Virol. 2018 Jan 2;92(2):e01582-17. [Abstract]
- Xenobiotica. 2019 Aug;49(8):935-944. [Abstract]
- J Liq ChromaTogr R T. 2019: 1-9.
- University of Glasgow. 2024 Mar.
- SSRN. 2024 Jan 26.
- Chemrxiv. 2021, Jun 10.
- Chem Cent J. 2017 Jan 3:11:1. [Abstract]
Activité biologique
Description
IC50 & Target
EC50: 0.82 to 19.3 pM (HCV genotypes 1 to 5), 366 pM (HCV genotype 6a)[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Huh-7 | CC50 |
>1 nM
Compound: Ombitasvir
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Cytotoxicity against human HuH7 replicon cells after 3 days by CellTiter-Fluor assay
Cytotoxicity against human HuH7 replicon cells after 3 days by CellTiter-Fluor assay
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[PMID: 29454920] |
| Huh-7 | EC50 |
0.005 nM
Compound: 38, ABT-267
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Antiviral activity against HCV1b Con1 infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay
Antiviral activity against HCV1b Con1 infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay
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[PMID: 24400777] |
| Huh-7 | EC50 |
0.014 nM
Compound: 38, ABT-267
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Antiviral activity against HCV1a H77 infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay
Antiviral activity against HCV1a H77 infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay
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[PMID: 24400777] |
| Huh-7 | EC50 |
0.056 nM
Compound: 38, ABT-267
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Antiviral activity against HCV1b Con1 infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay in presence of 40% human plasma
Antiviral activity against HCV1b Con1 infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay in presence of 40% human plasma
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[PMID: 24400777] |
| Huh-7 | EC50 |
0.186 nM
Compound: 38, ABT-267
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Antiviral activity against HCV1a H77 infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay in presence of 40% human plasma
Antiviral activity against HCV1a H77 infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay in presence of 40% human plasma
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[PMID: 24400777] |
| Huh-7 | EC50 |
1.71 pM
Compound: 38, ABT-267
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Antiviral activity against HCV4a infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay
Antiviral activity against HCV4a infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay
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[PMID: 24400777] |
| Huh-7 | EC50 |
12.4 pM
Compound: 38, ABT-267
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Antiviral activity against HCV2a infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay
Antiviral activity against HCV2a infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay
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[PMID: 24400777] |
| Huh-7 | EC50 |
19.3 pM
Compound: 38, ABT-267
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Antiviral activity against HCV3a infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay
Antiviral activity against HCV3a infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay
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[PMID: 24400777] |
| Huh-7 | EC50 |
4.3 pM
Compound: 38, ABT-267
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Antiviral activity against HCV2b infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay
Antiviral activity against HCV2b infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay
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[PMID: 24400777] |
| Huh-7 | EC50 |
4.3 pM
Compound: 38, ABT-267
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Antiviral activity against HCV5a infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay
Antiviral activity against HCV5a infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay
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[PMID: 24400777] |
| Huh-7 | EC50 |
415 pM
Compound: 38, ABT-267
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Antiviral activity against HCV6a infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay
Antiviral activity against HCV6a infected in human HuH7 cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene assay
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[PMID: 24400777] |
In Vitro
Ombitasvir is active against the genotype 2a JFH-1 replicon, with an EC50 of 0.82 pM, and the EC50s of ombitasvir are 42 pM and 68 pM against the L28 and F28 variants of this genotype 6a replicon, respectively[1]. In GT1a, variants M28V, L31V, and H58D confers 58- to 243-fold resistance to Ombitasvir. Single variants M28T, Q30R, and Y93C/S confers 800- to 8965-fold resistance, while Y93H/N confers >40000-fold resistance to Ombitasvir[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Essai clinique
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 1258226-87-7
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Appearance Solid
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Masse moléculaire 894.11
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Formule C50H67N7O8
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Color White to light yellow
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SMILES
CC(C)(C)C(C=C1)=CC=C1N2[C@H](C3=CC=C(NC([C@H]4N(C([C@@H](NC(OC)=O)C(C)C)=O)CCC4)=O)C=C3)CC[C@H]2C5=CC=C(NC([C@@H]6CCCN6C([C@@H](NC(OC)=O)C(C)C)=O)=O)C=C5
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Synonyms
ABT-267
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (9)
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Journal Impact Factor
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Most Recent
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Int J Mol Sci
Discovery of TRPV4-Targeting Small Molecules with Anti-Influenza Effects Through Machine Learning and Experimental Validation. [Abstract]2025 Feb 6;26(3):1381. PMID: 39941149 -
Antiviral Res
A profiling study of a newly developed HCVcc strain PR63cc's sensitivity to direct-acting antivirals. [Abstract]2017 Mar:139:18-24. PMID: 28025084 -
J Virol
2018 Jan 2;92(2):e01582-17. PMID: 29093094 -
Xenobiotica
Minor contribution of CYP3A5 to the metabolism of hepatitis C protease inhibitor paritaprevir in vitro. [Abstract]2019 Aug;49(8):935-944. PMID: 30227770 -
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Chem Cent J
Simultaneous determination of newly developed antiviral agents in pharmaceutical formulations by HPLC-DAD. [Abstract]2017 Jan 3:11:1. PMID: 28101128
Solvant et solubilité
In Vitro:
DMSO : ≥ 33 mg/mL (36.91 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.80 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Krishnan P, et al. In vitro and in vivo antiviral activity and resistance profile of ombitasvir, an inhibitor of hepatitis C virus NS5A. Antimicrob Agents Chemother. 2015 Feb;59(2):979-87 [Content Brief]
[2]. DeGoey DA, et al. Discovery of ABT-267, a pan-genotypic inhibitor of HCV NS5A. J Med Chem. 2014 Mar 13;57(5):2047-57 [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1184 mL | 5.5922 mL | 11.1843 mL | 27.9608 mL |
| 5 mM | 0.2237 mL | 1.1184 mL | 2.2369 mL | 5.5922 mL | |
| 10 mM | 0.1118 mL | 0.5592 mL | 1.1184 mL | 2.7961 mL | |
| 15 mM | 0.0746 mL | 0.3728 mL | 0.7456 mL | 1.8641 mL | |
| 20 mM | 0.0559 mL | 0.2796 mL | 0.5592 mL | 1.3980 mL | |
| 25 mM | 0.0447 mL | 0.2237 mL | 0.4474 mL | 1.1184 mL | |
| 30 mM | 0.0373 mL | 0.1864 mL | 0.3728 mL | 0.9320 mL |
Ombitasvir Related Classifications
HY-13997 Related Classifications
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