Amine Reactive Dyes

Cy2 Iodide is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.

6-TRITC (Tetramethylrhodamine-6-isothiocyanate) is a potent fluorescent tracer. 6-TRITC can be used as an probe for haptenated proteins/peptides for the research of contact allergy. 6-TRITC can be used as a tracer for the confocal imaging in living cells.

5(6)-Aminofluorescein (5(6)-AFM) is an amino-containing fluorescein dye primarily used for biomolecular labeling, fluorescence imaging, and as a precursor for the synthesis of fluorescent probes (Ex/Em = 495/515 nm).

7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester is the amine-reactive succinimidyl ester of 7-Hydroxycoumarin-3-carboxylic acid. 7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester is a blue fluorescent dye for labeling proteins and nucleic acids.

5(6)-SFX ,SE (Compound SFX-SE) is a fluorescent dye for labeling primary amines and nascent proteins.

AMCA-X-SE is a coumarin derivative that generates fixed blue fluorescence and an NHS-activated ester that forms stable amide bonds with primary amine groups. It is used as a reactive dye for labeling amino groups of peptides, proteins, and oligonucleotides. Maximum excitation/emission wavelength: 354/442 nm.

NHS-5(6)Carboxyrhodamine is a dye used for fluorescence labeling applications, where accurate dye/protein ratios can be obtained under native conditions.

Cy 3 Non-Sulfonated (Cyanine3) is a cyanine (Cy) dye, and a fluorescent label with green channel for protein and nucleic acid. Cy 3 Non-Sulfonated is a fluorescent photoproduct of Cyanine5 via photoconversion upon photoexcitation. Cy 3 Non-Sulfonated can be used to high-density single-particle tracking in a living cell without using UV illumination and cell-toxic additives (Ex=470 nm; Em=515 nm and 565 nm nm).

1,4-Dichloro 6-carboxytetramethylrhodamine is a fluorescence labeling agent (Ex=541 nm, Em=568 nm).

DNP-X acid (6-((2,4-Dinitrophenyl)amino)hexanoic acid), an amine-reactive building block for developing a probe, can be recognized by anti-DNP antibodies. DNP-X acid is also an excellent amine-reactive FRET quencher paired with Trp or Tyr.

Fluorescein-PEG6-amine is a derivative of Fluorescein. Fluorescein-PEG6-amine features free amine group for further conjugation. Fluorescein-PEG6-amine is commonly used in fluorescence microscopy, flow cytometry, immunoassays, and nucleic acid detection (Ex/Em = 495/519 nm) .

ATTO 655 NHS ester features an NHS ester reactive group used for conjugation and labeling via primary amine groups. It is primarily used for labeling antibodies and proteins (Ex/Em = 655/685 nm).

BODIPY 576/589 NHS ester (BODIPY 576/589 Succinimidyl ester) is an active fluorophore containing the BODIPY 576/589 fluorophore and an NHS ester. BODIPY 576/589 NHS ester can covalently couple red fluorescence to biomolecules containing primary amines (-NH2), such as proteins, antibodies, peptides, and oligonucleotides. BODIPY 576/589 NHS ester can also be used to prepare BODIPY 576/589-Met-tRNA_fMet to achieve N-terminal co-translational labeling of nascent proteins (Ex/Em = 576/589 nm).

Rhodamine 101 NHS Ester features an NHS ester reactive group used for conjugation and labeling via primary amine groups. It is primarily used for labeling antibodies and proteins (Ex/Em = 575/605 nm).

ROX amine (6-isomer) is a derivative of ROX (carboxy-X-rhodamine) featuring a free amino group, used for further conjugation and labeling (Ex/Em = 578/604 nm).

6-VIC NHS ester features an NHS ester reactive group used for conjugation and labeling via primary amine groups. It is primarily used for antibody/protein labeling (Ex/Em = 538/554 nm).

ATTO 495 NHS ester features an NHS ester reactive group used for conjugation and labeling via primary amine groups. It is primarily used for labeling antibodies and proteins (Ex/Em = 495/520 nm).

ATTO Rho 11 NHS este rfeatures an NHS ester reactive group used for conjugation and labeling via primary amine groups. It is primarily used for labeling antibodies and proteins (Ex/Em = 568/592 nm).

AF790 NHS ester is a derivative of Alexa Fluor 790 featuring an NHS ester reactive group, used for conjugation and labeling via primary amine groups (Ex/Em = 782/805 nm) .

ATTO Rho 101 NHS ester features an NHS ester reactive group used for conjugation and labeling via primary amine groups. It is primarily used for labeling antibodies and proteins (Ex/Em = 575/600 nm).