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Cat. No.: HY-D0167

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NHS-5(6)Carboxyrhodamine

NHS-5(6)Carboxyrhodamine is a dye used for fluorescence labeling applications, where accurate dye/protein ratios can be obtained under native conditions.

For research use only. We do not sell to patients.

CAS No.: 150408-83-6
Formula: C₂₉H₂₅N₃O₇
Molecular Weight:527.53

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (273 KB)

NHS-5(6)Carboxyrhodamine Chemical Structure

CAS No.: 150408-83-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

NHS-5(6)Carboxyrhodamine is a dye used for fluorescence labeling applications, where accurate dye/protein ratios can be obtained under native conditions^[1].

In Vitro

A general characteristic for all of the NHS-5(6)Carboxyrhodamine/ConA samples is that the 556 nm peak is significantly larger relative to the 520 nm peak^[1].
Dye/protein ratios for NHS-5(6)Carboxyrhodamine under native conditions are close to the ratios obtained under denaturing conditions^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

515

Excitation (Ex)

488

Chemical Information

CAS No. 150408-83-6
Molecular Weight 527.53
Formula C₂₉H₂₅N₃O₇
SMILES

O=C1OC2(C3=C(OC4=C2C=CC(N(C)C)=C4)C=C(N(C)C)C=C3)C5=C1C=CC=C5.CC(ON6C(CCC6=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. D L Meadows, et al. Determining the extent of labeling for tetramethylrhodamine protein conjugates. J Immunol Methods. 1991 Oct 25;143(2):263-72. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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