FITC-Chitosan (MW 100000) 

FITC-Chitosan (MW 100000) is a chitosan (Chitosan) (HY-B2144A) labeled with FITC (HY-66019). FITC-Chitosan (MW 100000) combines the biological and physicochemical properties of chitosan (such as biocompatibility, positive charge, and nanoparticle-forming ability) with the fluorescent visibility of FITC (Ex/Em = ~485/535 nm)^[1][2][3].

FITC-Chitosan (high molecular weight, e.g., 300 kDa, 18 kDa) can be used to prepare fluorescence-labeled nanoparticles and investigate cellular uptake and in vivo biodistribution of nanoparticles^[1][3].

Guide (the following is a recommended protocol for reference only, which can be adjusted according to actual requirements).
1. Cell culture: Seed cells in multi-well plates or confocal culture dishes, and culture them in medium containing 10% FBS until reaching 70-90% confluency.
2. Experimental procedures:
2.1 Wash cells with PBS or serum-free medium. Add fresh serum-free medium containing FITC-Chitosan or its nanoparticles at different concentrations.
Key controls: Set up two temperature groups, 37°C (allowing internalization) and 4°C (inhibiting internalization, mainly investigating surface adsorption).
2.2 Incubation: Incubate cells in a cell incubator for 0.5 to 4 hours (determine the linear uptake phase according to pre-experiments).
2.3 Termination and washing: Immediately after incubation, wash cells 3-4 times with pre-cooled PBS to terminate the reaction and remove unbound nanoparticles.
2.4 Membrane surface fluorescence quenching (optional but recommended): To distinguish FITC-Chitosan bound to the cell surface from internalized FITC-Chitosan, digest cells with 0.25% trypsin at 37°C for 30 minutes to remove surface-bound probes, followed by another wash with PBS.
3. Cell lysis and fluorescence quantification:
3.1 Add 0.2 N NaOH solution containing 0.5% Triton X-100 to lyse cells.
3.2 Transfer the cell lysate to a black opaque 96-well plate.
Measure the fluorescence intensity using a fluorescence microplate reader at Ex/Em = ~485/535 nm.
3.3 Convert the fluorescence value to the mass of FITC-Chitosan taken up by cells via a FITC-Chitosan standard curve (prepared in the same lysate).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

100000 (Average)

Solid

yellow

OC1=CC2=C(C3(OC(C4=CC(C(N[C@@H]([C@H](O)O[C@@H]5CO)C(O)[C@@H]5O[C@H]6[C@H](N)[C@H](O)[C@H](O)[C@@H](CO)O6)=O)=CC=C43)=O)C7=C(C=C(C=C7)O)O2)C=C1.[n]

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

• [1]. Huang M, et al. Uptake of FITC-chitosan nanoparticles by A549 cells. Pharm Res. 2002;19(10):1488-1494.  [Content Brief]

  [2]. Cheng X, et al. DNA/chitosan nanocomplex as a novel drug carrier for doxorubicin. Drug Deliv. 2009;16(3):135-144.  [Content Brief]

  [3]. Yan L, et al. Small interfering RNA-loaded chitosan hydrochloride/carboxymethyl chitosan nanoparticles for ultrasound-triggered release to hamper colorectal cancer growth in vitro. Int J Biol Macromol. 2020;162:1303-1310.  [Content Brief]