Fluorescein diphosphate tetraammonium

Fluorescein diphosphate tetraammonium can be used as a fluorogenic substrate, and obtains luorescein as a fluorescent product^[1].
Fluorescein diphosphate tetraammonium (50 μM; room temperature) can be used as a substrate in vitro phosphatase assays^[2].
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
ELISA with capillary immunosensor (single-step ELISA)^[3]:
1. Put various concentrations of antigen in Tris buffer containing 1% BSA into capillary immunosensor.
2. Immobilize capture antibody and enzyme-linked antibody via capillary action, incubates for a preset time, and subsequently washing with 1 mL of 50 mM Tris buffer (pH 7.4) containing 0.14 M NaCl and 0.05% Tween 20 by using a syringe.
3. Fluorescence is detected by introducing an excess volume (ca. 100 μL) of 20 μM FDP in 50 mM Tris buffer (pH 9) with an incubation time of 60 min.
4. Fluorescence detection analysis

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.